Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13255 and RPSI07_RS13260 are separated by 140 nucleotides RPSI07_RS13260 and RPSI07_RS13265 overlap by 98 nucleotides RPSI07_RS13265 and RPSI07_RS13270 overlap by 70 nucleotides RPSI07_RS13270 and RPSI07_RS13275 are separated by 495 nucleotides
RPSI07_RS13255: RPSI07_RS13255 - hypothetical protein, at 1,160,378 to 1,160,617
_RS13255
RPSI07_RS13260: RPSI07_RS13260 - MerR family transcriptional regulator, at 1,160,758 to 1,161,288
_RS13260
RPSI07_RS13265: RPSI07_RS13265 - carboxymuconolactone decarboxylase, at 1,161,191 to 1,161,670
_RS13265
RPSI07_RS13270: RPSI07_RS13270 - HlyC/CorC family transporter, at 1,161,601 to 1,162,926
_RS13270
RPSI07_RS13275: RPSI07_RS13275 - hypothetical protein, at 1,163,422 to 1,166,271
_RS13275
Position (kb)
1161
1162
1163 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1160.739 kb on + strand at 1160.746 kb on + strand at 1160.746 kb on + strand at 1160.746 kb on + strand at 1160.746 kb on + strand at 1160.746 kb on + strand at 1160.747 kb on - strand at 1160.747 kb on - strand at 1160.747 kb on - strand at 1160.828 kb on - strand, within RPSI07_RS13260 at 1161.109 kb on + strand, within RPSI07_RS13260 at 1161.110 kb on - strand, within RPSI07_RS13260 at 1161.110 kb on - strand, within RPSI07_RS13260 at 1161.110 kb on - strand, within RPSI07_RS13260 at 1161.110 kb on - strand, within RPSI07_RS13260 at 1161.252 kb on - strand at 1161.347 kb on + strand, within RPSI07_RS13265 at 1161.347 kb on + strand, within RPSI07_RS13265 at 1161.347 kb on + strand, within RPSI07_RS13265 at 1161.347 kb on + strand, within RPSI07_RS13265 at 1161.347 kb on + strand, within RPSI07_RS13265 at 1161.348 kb on - strand, within RPSI07_RS13265 at 1161.349 kb on + strand, within RPSI07_RS13265 at 1161.349 kb on + strand, within RPSI07_RS13265 at 1161.349 kb on + strand, within RPSI07_RS13265 at 1161.377 kb on + strand, within RPSI07_RS13265 at 1161.377 kb on + strand, within RPSI07_RS13265 at 1161.378 kb on - strand, within RPSI07_RS13265 at 1161.378 kb on - strand, within RPSI07_RS13265 at 1161.378 kb on - strand, within RPSI07_RS13265 at 1161.379 kb on - strand, within RPSI07_RS13265 at 1161.542 kb on + strand, within RPSI07_RS13265 at 1161.542 kb on + strand, within RPSI07_RS13265 at 1161.543 kb on - strand, within RPSI07_RS13265 at 1161.761 kb on + strand, within RPSI07_RS13270 at 1161.761 kb on + strand, within RPSI07_RS13270 at 1161.762 kb on - strand, within RPSI07_RS13270 at 1161.762 kb on - strand, within RPSI07_RS13270 at 1161.911 kb on + strand, within RPSI07_RS13270 at 1161.981 kb on - strand, within RPSI07_RS13270 at 1162.080 kb on - strand, within RPSI07_RS13270 at 1162.196 kb on + strand, within RPSI07_RS13270 at 1162.197 kb on - strand, within RPSI07_RS13270 at 1162.262 kb on + strand, within RPSI07_RS13270 at 1162.263 kb on - strand, within RPSI07_RS13270 at 1162.551 kb on - strand, within RPSI07_RS13270 at 1162.555 kb on + strand, within RPSI07_RS13270 at 1162.607 kb on - strand, within RPSI07_RS13270 at 1162.922 kb on + strand at 1162.922 kb on + strand at 1162.922 kb on + strand at 1162.922 kb on + strand at 1162.922 kb on + strand at 1162.922 kb on + strand at 1162.923 kb on - strand at 1162.923 kb on - strand at 1162.923 kb on - strand at 1162.923 kb on - strand at 1162.923 kb on - strand at 1162.923 kb on - strand at 1162.923 kb on - strand at 1162.923 kb on - strand at 1162.923 kb on - strand at 1162.926 kb on + strand at 1162.926 kb on + strand at 1162.926 kb on + strand at 1162.927 kb on - strand at 1162.927 kb on - strand at 1162.927 kb on - strand at 1162.927 kb on - strand at 1163.149 kb on - strand at 1163.217 kb on - strand at 1163.326 kb on + strand at 1163.326 kb on + strand at 1163.330 kb on - strand at 1163.330 kb on - strand at 1163.347 kb on + strand at 1163.347 kb on + strand at 1163.348 kb on - strand at 1163.348 kb on - strand at 1163.348 kb on - strand at 1163.348 kb on - strand at 1163.348 kb on - strand at 1163.348 kb on - strand at 1163.369 kb on + strand at 1163.369 kb on + strand at 1163.370 kb on - strand at 1163.569 kb on + strand at 1163.569 kb on + strand at 1163.570 kb on - strand at 1163.722 kb on + strand, within RPSI07_RS13275 at 1163.723 kb on - strand, within RPSI07_RS13275 at 1163.815 kb on + strand, within RPSI07_RS13275 at 1163.816 kb on - strand, within RPSI07_RS13275 at 1163.816 kb on - strand, within RPSI07_RS13275 at 1163.888 kb on - strand, within RPSI07_RS13275 at 1163.888 kb on - strand, within RPSI07_RS13275
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 1,160,739 + -0.8 1,160,746 + +1.1 1,160,746 + -1.7 1,160,746 + -0.9 1,160,746 + -0.8 1,160,746 + -1.1 1,160,747 - +0.0 1,160,747 - +0.9 1,160,747 - -0.9 1,160,828 - RPSI07_RS13260 0.13 +0.7 1,161,109 + RPSI07_RS13260 0.66 -0.3 1,161,110 - RPSI07_RS13260 0.66 -0.1 1,161,110 - RPSI07_RS13260 0.66 -0.2 1,161,110 - RPSI07_RS13260 0.66 -0.4 1,161,110 - RPSI07_RS13260 0.66 +0.5 1,161,252 - +0.2 1,161,347 + RPSI07_RS13265 0.33 -1.1 1,161,347 + RPSI07_RS13265 0.33 -0.3 1,161,347 + RPSI07_RS13265 0.33 -1.9 1,161,347 + RPSI07_RS13265 0.33 +0.0 1,161,347 + RPSI07_RS13265 0.33 -2.0 1,161,348 - RPSI07_RS13265 0.33 +0.7 1,161,349 + RPSI07_RS13265 0.33 -0.1 1,161,349 + RPSI07_RS13265 0.33 -2.0 1,161,349 + RPSI07_RS13265 0.33 +0.1 1,161,377 + RPSI07_RS13265 0.39 -1.6 1,161,377 + RPSI07_RS13265 0.39 -1.7 1,161,378 - RPSI07_RS13265 0.39 -1.2 1,161,378 - RPSI07_RS13265 0.39 +1.8 1,161,378 - RPSI07_RS13265 0.39 +0.8 1,161,379 - RPSI07_RS13265 0.39 +0.1 1,161,542 + RPSI07_RS13265 0.73 +4.7 1,161,542 + RPSI07_RS13265 0.73 +0.5 1,161,543 - RPSI07_RS13265 0.73 -0.2 1,161,761 + RPSI07_RS13270 0.12 +1.8 1,161,761 + RPSI07_RS13270 0.12 -2.6 1,161,762 - RPSI07_RS13270 0.12 +2.2 1,161,762 - RPSI07_RS13270 0.12 -0.6 1,161,911 + RPSI07_RS13270 0.23 -0.3 1,161,981 - RPSI07_RS13270 0.29 -0.7 1,162,080 - RPSI07_RS13270 0.36 -0.6 1,162,196 + RPSI07_RS13270 0.45 -1.3 1,162,197 - RPSI07_RS13270 0.45 -0.3 1,162,262 + RPSI07_RS13270 0.50 -1.0 1,162,263 - RPSI07_RS13270 0.50 -2.6 1,162,551 - RPSI07_RS13270 0.72 +1.2 1,162,555 + RPSI07_RS13270 0.72 +0.4 1,162,607 - RPSI07_RS13270 0.76 -0.1 1,162,922 + -1.6 1,162,922 + -0.2 1,162,922 + +0.7 1,162,922 + +1.2 1,162,922 + +0.6 1,162,922 + +0.2 1,162,923 - -0.3 1,162,923 - +1.1 1,162,923 - +0.1 1,162,923 - +0.2 1,162,923 - -1.2 1,162,923 - +0.7 1,162,923 - -0.6 1,162,923 - -0.5 1,162,923 - -0.6 1,162,926 + -1.8 1,162,926 + -0.1 1,162,926 + -0.1 1,162,927 - -0.5 1,162,927 - -1.8 1,162,927 - +0.5 1,162,927 - +0.2 1,163,149 - -0.3 1,163,217 - +1.0 1,163,326 + +0.6 1,163,326 + +1.2 1,163,330 - +1.2 1,163,330 - +1.2 1,163,347 + +0.4 1,163,347 + -1.1 1,163,348 - +0.2 1,163,348 - -2.5 1,163,348 - +0.0 1,163,348 - -0.9 1,163,348 - -0.3 1,163,348 - -0.9 1,163,369 + -0.9 1,163,369 + +0.9 1,163,370 - -0.7 1,163,569 + -0.2 1,163,569 + +0.9 1,163,570 - +0.4 1,163,722 + RPSI07_RS13275 0.11 -1.7 1,163,723 - RPSI07_RS13275 0.11 -0.7 1,163,815 + RPSI07_RS13275 0.14 -2.1 1,163,816 - RPSI07_RS13275 0.14 +0.1 1,163,816 - RPSI07_RS13275 0.14 +0.5 1,163,888 - RPSI07_RS13275 0.16 +0.1 1,163,888 - RPSI07_RS13275 0.16 +0.8
Or see this region's nucleotide sequence