Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10745 and RPSI07_RS10750 overlap by 1 nucleotides RPSI07_RS10750 and RPSI07_RS10755 are separated by 17 nucleotides RPSI07_RS10755 and RPSI07_RS10760 are separated by 366 nucleotides
RPSI07_RS10745: RPSI07_RS10745 - DUF1311 domain-containing protein, at 610,312 to 610,749
_RS10745
RPSI07_RS10750: RPSI07_RS10750 - type 1 glutamine amidotransferase, at 610,749 to 611,318
_RS10750
RPSI07_RS10755: RPSI07_RS10755 - anthranilate synthase component I, at 611,336 to 612,868
_RS10755
RPSI07_RS10760: RPSI07_RS10760 - phosphoglycolate phosphatase, at 613,235 to 613,975
_RS10760
Position (kb) 611
612
613 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 610.476 kb on - strand, within RPSI07_RS10745 at 610.665 kb on + strand, within RPSI07_RS10745 at 611.330 kb on + strand at 611.331 kb on - strand at 611.331 kb on - strand at 611.338 kb on - strand at 612.769 kb on - strand at 613.143 kb on + strand at 613.144 kb on - strand at 613.328 kb on + strand, within RPSI07_RS10760 at 613.329 kb on - strand, within RPSI07_RS10760 at 613.330 kb on + strand, within RPSI07_RS10760 at 613.331 kb on - strand, within RPSI07_RS10760 at 613.331 kb on - strand, within RPSI07_RS10760 at 613.331 kb on - strand, within RPSI07_RS10760 at 613.352 kb on + strand, within RPSI07_RS10760 at 613.353 kb on - strand, within RPSI07_RS10760 at 613.371 kb on + strand, within RPSI07_RS10760 at 613.372 kb on - strand, within RPSI07_RS10760 at 613.372 kb on - strand, within RPSI07_RS10760 at 613.377 kb on + strand, within RPSI07_RS10760 at 613.540 kb on - strand, within RPSI07_RS10760 at 613.674 kb on + strand, within RPSI07_RS10760 at 613.675 kb on - strand, within RPSI07_RS10760 at 613.675 kb on - strand, within RPSI07_RS10760 at 613.707 kb on + strand, within RPSI07_RS10760 at 613.707 kb on + strand, within RPSI07_RS10760 at 613.708 kb on - strand, within RPSI07_RS10760 at 613.708 kb on - strand, within RPSI07_RS10760 at 613.708 kb on - strand, within RPSI07_RS10760 at 613.819 kb on - strand, within RPSI07_RS10760
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 610,476 - RPSI07_RS10745 0.37 +0.3 610,665 + RPSI07_RS10745 0.81 -1.2 611,330 + -0.2 611,331 - -0.9 611,331 - +0.3 611,338 - -1.0 612,769 - +0.7 613,143 + +0.8 613,144 - -0.9 613,328 + RPSI07_RS10760 0.13 +1.4 613,329 - RPSI07_RS10760 0.13 -1.5 613,330 + RPSI07_RS10760 0.13 -2.9 613,331 - RPSI07_RS10760 0.13 -0.3 613,331 - RPSI07_RS10760 0.13 +0.1 613,331 - RPSI07_RS10760 0.13 -1.2 613,352 + RPSI07_RS10760 0.16 -1.6 613,353 - RPSI07_RS10760 0.16 +1.4 613,371 + RPSI07_RS10760 0.18 -0.5 613,372 - RPSI07_RS10760 0.18 -0.3 613,372 - RPSI07_RS10760 0.18 +1.4 613,377 + RPSI07_RS10760 0.19 -0.8 613,540 - RPSI07_RS10760 0.41 +0.8 613,674 + RPSI07_RS10760 0.59 -0.1 613,675 - RPSI07_RS10760 0.59 +0.3 613,675 - RPSI07_RS10760 0.59 +2.0 613,707 + RPSI07_RS10760 0.64 -0.1 613,707 + RPSI07_RS10760 0.64 +0.4 613,708 - RPSI07_RS10760 0.64 -0.0 613,708 - RPSI07_RS10760 0.64 -2.9 613,708 - RPSI07_RS10760 0.64 -1.6 613,819 - RPSI07_RS10760 0.79 -0.1
Or see this region's nucleotide sequence