Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10535 and RPSI07_RS10540 are separated by 13 nucleotides RPSI07_RS10540 and RPSI07_RS10545 are separated by 531 nucleotides
RPSI07_RS10535: RPSI07_RS10535 - sensor histidine kinase, at 563,514 to 564,812
_RS10535
RPSI07_RS10540: RPSI07_RS10540 - TonB-denpendent receptor, at 564,826 to 566,925
_RS10540
RPSI07_RS10545: RPSI07_RS10545 - gamma-glutamyltransferase family protein, at 567,457 to 569,325
_RS10545
Position (kb) 564
565
566
567 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 564.577 kb on - strand, within RPSI07_RS10535 at 564.769 kb on - strand at 564.769 kb on - strand at 564.769 kb on - strand at 564.769 kb on - strand at 564.953 kb on + strand at 564.954 kb on - strand at 565.034 kb on + strand at 565.075 kb on + strand, within RPSI07_RS10540 at 565.075 kb on + strand, within RPSI07_RS10540 at 565.075 kb on + strand, within RPSI07_RS10540 at 565.076 kb on - strand, within RPSI07_RS10540 at 565.076 kb on - strand, within RPSI07_RS10540 at 565.076 kb on - strand, within RPSI07_RS10540 at 565.076 kb on - strand, within RPSI07_RS10540 at 565.076 kb on - strand, within RPSI07_RS10540 at 565.113 kb on - strand, within RPSI07_RS10540 at 565.196 kb on + strand, within RPSI07_RS10540 at 565.196 kb on + strand at 565.196 kb on + strand, within RPSI07_RS10540 at 565.197 kb on - strand, within RPSI07_RS10540 at 565.197 kb on - strand, within RPSI07_RS10540 at 565.197 kb on - strand, within RPSI07_RS10540 at 565.280 kb on + strand, within RPSI07_RS10540 at 565.280 kb on + strand, within RPSI07_RS10540 at 565.280 kb on + strand, within RPSI07_RS10540 at 565.281 kb on - strand, within RPSI07_RS10540 at 565.462 kb on + strand, within RPSI07_RS10540 at 565.463 kb on - strand, within RPSI07_RS10540 at 565.614 kb on + strand, within RPSI07_RS10540 at 565.614 kb on + strand, within RPSI07_RS10540 at 565.614 kb on + strand, within RPSI07_RS10540 at 565.615 kb on - strand, within RPSI07_RS10540 at 565.615 kb on - strand, within RPSI07_RS10540 at 565.736 kb on + strand, within RPSI07_RS10540 at 565.737 kb on - strand, within RPSI07_RS10540 at 565.784 kb on + strand, within RPSI07_RS10540 at 565.976 kb on + strand, within RPSI07_RS10540 at 565.976 kb on + strand, within RPSI07_RS10540 at 565.976 kb on + strand, within RPSI07_RS10540 at 565.977 kb on - strand, within RPSI07_RS10540 at 565.977 kb on - strand, within RPSI07_RS10540 at 565.977 kb on - strand, within RPSI07_RS10540 at 565.977 kb on - strand, within RPSI07_RS10540 at 566.018 kb on + strand, within RPSI07_RS10540 at 566.019 kb on - strand, within RPSI07_RS10540 at 566.078 kb on + strand, within RPSI07_RS10540 at 566.078 kb on + strand, within RPSI07_RS10540 at 566.238 kb on + strand, within RPSI07_RS10540 at 566.238 kb on + strand, within RPSI07_RS10540 at 566.239 kb on - strand, within RPSI07_RS10540 at 566.319 kb on - strand, within RPSI07_RS10540 at 566.378 kb on + strand, within RPSI07_RS10540 at 566.426 kb on + strand, within RPSI07_RS10540 at 566.427 kb on - strand, within RPSI07_RS10540 at 566.427 kb on - strand, within RPSI07_RS10540 at 566.427 kb on - strand, within RPSI07_RS10540 at 566.477 kb on + strand, within RPSI07_RS10540 at 566.477 kb on + strand, within RPSI07_RS10540 at 566.478 kb on - strand, within RPSI07_RS10540 at 566.478 kb on - strand, within RPSI07_RS10540 at 566.492 kb on + strand, within RPSI07_RS10540 at 566.492 kb on + strand, within RPSI07_RS10540 at 566.492 kb on + strand, within RPSI07_RS10540 at 566.541 kb on - strand, within RPSI07_RS10540 at 566.541 kb on - strand, within RPSI07_RS10540 at 566.639 kb on + strand, within RPSI07_RS10540 at 566.640 kb on - strand, within RPSI07_RS10540 at 566.753 kb on + strand at 566.753 kb on + strand at 566.754 kb on - strand at 566.967 kb on + strand at 566.967 kb on + strand at 566.967 kb on + strand at 567.065 kb on + strand at 567.065 kb on + strand at 567.065 kb on + strand at 567.065 kb on + strand at 567.065 kb on + strand at 567.066 kb on - strand at 567.066 kb on - strand at 567.066 kb on - strand at 567.066 kb on - strand at 567.761 kb on + strand, within RPSI07_RS10545 at 567.818 kb on - strand, within RPSI07_RS10545 at 567.818 kb on - strand, within RPSI07_RS10545 at 567.845 kb on - strand, within RPSI07_RS10545
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 564,577 - RPSI07_RS10535 0.82 -0.0 564,769 - -0.2 564,769 - -0.6 564,769 - -0.7 564,769 - -0.1 564,953 + +0.3 564,954 - +0.2 565,034 + +0.4 565,075 + RPSI07_RS10540 0.12 +0.1 565,075 + RPSI07_RS10540 0.12 -0.9 565,075 + RPSI07_RS10540 0.12 -0.2 565,076 - RPSI07_RS10540 0.12 +0.8 565,076 - RPSI07_RS10540 0.12 +0.8 565,076 - RPSI07_RS10540 0.12 +2.4 565,076 - RPSI07_RS10540 0.12 +1.4 565,076 - RPSI07_RS10540 0.12 +1.7 565,113 - RPSI07_RS10540 0.14 +1.4 565,196 + RPSI07_RS10540 0.18 +0.6 565,196 + +3.0 565,196 + RPSI07_RS10540 0.18 +0.4 565,197 - RPSI07_RS10540 0.18 +0.1 565,197 - RPSI07_RS10540 0.18 -1.0 565,197 - RPSI07_RS10540 0.18 -0.1 565,280 + RPSI07_RS10540 0.22 -3.7 565,280 + RPSI07_RS10540 0.22 +0.6 565,280 + RPSI07_RS10540 0.22 +0.1 565,281 - RPSI07_RS10540 0.22 -2.7 565,462 + RPSI07_RS10540 0.30 -1.0 565,463 - RPSI07_RS10540 0.30 +1.2 565,614 + RPSI07_RS10540 0.38 -0.3 565,614 + RPSI07_RS10540 0.38 +1.4 565,614 + RPSI07_RS10540 0.38 +1.4 565,615 - RPSI07_RS10540 0.38 -0.6 565,615 - RPSI07_RS10540 0.38 -1.1 565,736 + RPSI07_RS10540 0.43 +2.1 565,737 - RPSI07_RS10540 0.43 +2.4 565,784 + RPSI07_RS10540 0.46 -0.4 565,976 + RPSI07_RS10540 0.55 -2.5 565,976 + RPSI07_RS10540 0.55 -0.1 565,976 + RPSI07_RS10540 0.55 +0.4 565,977 - RPSI07_RS10540 0.55 +0.7 565,977 - RPSI07_RS10540 0.55 +1.4 565,977 - RPSI07_RS10540 0.55 +0.4 565,977 - RPSI07_RS10540 0.55 -0.6 566,018 + RPSI07_RS10540 0.57 +0.1 566,019 - RPSI07_RS10540 0.57 +0.6 566,078 + RPSI07_RS10540 0.60 +0.1 566,078 + RPSI07_RS10540 0.60 -1.2 566,238 + RPSI07_RS10540 0.67 +3.4 566,238 + RPSI07_RS10540 0.67 +1.7 566,239 - RPSI07_RS10540 0.67 -0.1 566,319 - RPSI07_RS10540 0.71 -2.5 566,378 + RPSI07_RS10540 0.74 -1.1 566,426 + RPSI07_RS10540 0.76 -0.1 566,427 - RPSI07_RS10540 0.76 +1.4 566,427 - RPSI07_RS10540 0.76 +0.6 566,427 - RPSI07_RS10540 0.76 +0.8 566,477 + RPSI07_RS10540 0.79 +0.3 566,477 + RPSI07_RS10540 0.79 +1.0 566,478 - RPSI07_RS10540 0.79 -2.3 566,478 - RPSI07_RS10540 0.79 +0.1 566,492 + RPSI07_RS10540 0.79 -0.1 566,492 + RPSI07_RS10540 0.79 -1.0 566,492 + RPSI07_RS10540 0.79 +0.4 566,541 - RPSI07_RS10540 0.82 -0.0 566,541 - RPSI07_RS10540 0.82 -0.0 566,639 + RPSI07_RS10540 0.86 -1.3 566,640 - RPSI07_RS10540 0.86 +1.4 566,753 + -0.8 566,753 + -2.5 566,754 - +0.9 566,967 + +0.4 566,967 + -1.1 566,967 + -0.8 567,065 + -0.3 567,065 + +0.6 567,065 + -2.5 567,065 + +0.4 567,065 + -0.1 567,066 - +0.8 567,066 - -0.1 567,066 - +0.9 567,066 - +0.0 567,761 + RPSI07_RS10545 0.16 +0.6 567,818 - RPSI07_RS10545 0.19 +0.4 567,818 - RPSI07_RS10545 0.19 -0.6 567,845 - RPSI07_RS10545 0.21 +2.4
Or see this region's nucleotide sequence