Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10355 and RPSI07_RS10360 are separated by 65 nucleotides RPSI07_RS10360 and RPSI07_RS10365 are separated by 236 nucleotides RPSI07_RS10365 and RPSI07_RS10370 overlap by 4 nucleotides RPSI07_RS10370 and RPSI07_RS10375 are separated by 57 nucleotides
RPSI07_RS10355: RPSI07_RS10355 - hypothetical protein, at 532,394 to 533,029
_RS10355
RPSI07_RS10360: RPSI07_RS10360 - STAS domain-containing protein, at 533,095 to 533,409
_RS10360
RPSI07_RS10365: RPSI07_RS10365 - ABC transporter ATP-binding protein, at 533,646 to 534,611
_RS10365
RPSI07_RS10370: RPSI07_RS10370 - ABC transporter permease, at 534,608 to 535,396
_RS10370
RPSI07_RS10375: RPSI07_RS10375 - BolA family transcriptional regulator, at 535,454 to 535,690
_RS10375
Position (kb) 533
534
535 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 532.761 kb on - strand, within RPSI07_RS10355 at 532.883 kb on + strand, within RPSI07_RS10355 at 533.027 kb on + strand at 533.028 kb on - strand at 533.028 kb on - strand at 533.042 kb on + strand at 533.342 kb on - strand, within RPSI07_RS10360 at 533.342 kb on - strand, within RPSI07_RS10360 at 533.539 kb on - strand at 533.552 kb on + strand at 533.635 kb on + strand at 533.635 kb on + strand at 533.637 kb on + strand at 533.637 kb on + strand at 534.216 kb on + strand, within RPSI07_RS10365 at 534.216 kb on + strand, within RPSI07_RS10365 at 534.216 kb on + strand, within RPSI07_RS10365 at 535.087 kb on + strand, within RPSI07_RS10370 at 535.190 kb on + strand, within RPSI07_RS10370 at 535.190 kb on + strand, within RPSI07_RS10370 at 535.250 kb on + strand, within RPSI07_RS10370 at 535.405 kb on + strand at 535.484 kb on + strand, within RPSI07_RS10375
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 532,761 - RPSI07_RS10355 0.58 -0.6 532,883 + RPSI07_RS10355 0.77 -1.4 533,027 + +0.0 533,028 - -1.2 533,028 - -1.3 533,042 + -1.0 533,342 - RPSI07_RS10360 0.78 -1.9 533,342 - RPSI07_RS10360 0.78 -1.4 533,539 - +1.4 533,552 + -0.7 533,635 + +0.0 533,635 + -1.7 533,637 + +1.0 533,637 + +1.1 534,216 + RPSI07_RS10365 0.59 -0.0 534,216 + RPSI07_RS10365 0.59 -0.3 534,216 + RPSI07_RS10365 0.59 +0.7 535,087 + RPSI07_RS10370 0.61 -1.1 535,190 + RPSI07_RS10370 0.74 -0.4 535,190 + RPSI07_RS10370 0.74 -0.1 535,250 + RPSI07_RS10370 0.81 +0.4 535,405 + +2.3 535,484 + RPSI07_RS10375 0.13 +3.0
Or see this region's nucleotide sequence