Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS23575

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS23565 and RPSI07_RS23570 overlap by 4 nucleotidesRPSI07_RS23570 and RPSI07_RS23575 are separated by 13 nucleotidesRPSI07_RS23575 and RPSI07_RS23580 are separated by 94 nucleotides RPSI07_RS23565: RPSI07_RS23565 - PAS domain-containing sensor histidine kinase, at 3,427,748 to 3,430,183 _RS23565 RPSI07_RS23570: RPSI07_RS23570 - DUF4390 domain-containing protein, at 3,430,180 to 3,430,980 _RS23570 RPSI07_RS23575: RPSI07_RS23575 - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 3,430,994 to 3,432,319 _RS23575 RPSI07_RS23580: RPSI07_RS23580 - zinc metalloprotease HtpX, at 3,432,414 to 3,433,274 _RS23580 Position (kb) 3430 3431 3432 3433Strain fitness (log2 ratio) -2 -1 0 1 2at 3430.075 kb on + strandat 3430.164 kb on + strandat 3430.165 kb on - strandat 3430.165 kb on - strandat 3431.020 kb on - strandat 3431.023 kb on - strandat 3431.023 kb on - strandat 3431.124 kb on - strandat 3431.124 kb on - strandat 3431.199 kb on + strand, within RPSI07_RS23575at 3431.200 kb on - strand, within RPSI07_RS23575at 3431.200 kb on - strand, within RPSI07_RS23575at 3431.200 kb on - strand, within RPSI07_RS23575at 3431.234 kb on + strand, within RPSI07_RS23575at 3431.235 kb on - strand, within RPSI07_RS23575at 3431.745 kb on + strand, within RPSI07_RS23575at 3431.838 kb on + strand, within RPSI07_RS23575at 3431.874 kb on + strand, within RPSI07_RS23575at 3431.875 kb on - strand, within RPSI07_RS23575at 3432.049 kb on + strand, within RPSI07_RS23575at 3432.165 kb on + strand, within RPSI07_RS23575at 3432.396 kb on + strandat 3432.397 kb on - strandat 3432.397 kb on - strandat 3432.397 kb on - strandat 3432.397 kb on - strandat 3432.397 kb on - strandat 3432.397 kb on - strandat 3432.398 kb on + strandat 3432.398 kb on + strandat 3432.399 kb on - strandat 3432.399 kb on - strandat 3432.425 kb on - strandat 3432.590 kb on - strand, within RPSI07_RS23580at 3433.010 kb on - strand, within RPSI07_RS23580at 3433.063 kb on + strand, within RPSI07_RS23580at 3433.105 kb on + strand, within RPSI07_RS23580at 3433.105 kb on + strand, within RPSI07_RS23580at 3433.105 kb on + strand, within RPSI07_RS23580at 3433.106 kb on - strand, within RPSI07_RS23580at 3433.314 kb on + strandat 3433.314 kb on + strandat 3433.314 kb on + strandat 3433.314 kb on + strandat 3433.314 kb on + strandat 3433.315 kb on - strandat 3433.315 kb on - strandat 3433.315 kb on - strandat 3433.315 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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3,430,075 + +1.8
3,430,164 + +0.6
3,430,165 - -0.6
3,430,165 - +2.1
3,431,020 - -0.7
3,431,023 - +0.8
3,431,023 - -0.4
3,431,124 - +1.5
3,431,124 - -0.6
3,431,199 + RPSI07_RS23575 0.15 +0.2
3,431,200 - RPSI07_RS23575 0.16 -0.4
3,431,200 - RPSI07_RS23575 0.16 -0.0
3,431,200 - RPSI07_RS23575 0.16 +0.2
3,431,234 + RPSI07_RS23575 0.18 -0.2
3,431,235 - RPSI07_RS23575 0.18 -0.7
3,431,745 + RPSI07_RS23575 0.57 -0.0
3,431,838 + RPSI07_RS23575 0.64 +1.8
3,431,874 + RPSI07_RS23575 0.66 +1.8
3,431,875 - RPSI07_RS23575 0.66 +0.6
3,432,049 + RPSI07_RS23575 0.80 +0.3
3,432,165 + RPSI07_RS23575 0.88 -0.0
3,432,396 + -0.2
3,432,397 - -0.1
3,432,397 - +0.2
3,432,397 - -0.4
3,432,397 - +0.2
3,432,397 - -0.2
3,432,397 - +0.3
3,432,398 + -1.3
3,432,398 + -0.2
3,432,399 - +0.2
3,432,399 - +0.4
3,432,425 - -2.4
3,432,590 - RPSI07_RS23580 0.20 -1.4
3,433,010 - RPSI07_RS23580 0.69 +0.3
3,433,063 + RPSI07_RS23580 0.75 -0.2
3,433,105 + RPSI07_RS23580 0.80 -0.6
3,433,105 + RPSI07_RS23580 0.80 -1.3
3,433,105 + RPSI07_RS23580 0.80 +0.4
3,433,106 - RPSI07_RS23580 0.80 -1.8
3,433,314 + +1.2
3,433,314 + -1.3
3,433,314 + -0.9
3,433,314 + -0.2
3,433,314 + +0.1
3,433,315 - -1.7
3,433,315 - +0.1
3,433,315 - -0.2
3,433,315 - -0.4

Or see this region's nucleotide sequence