Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS23555 and RPSI07_RS23560 are separated by 64 nucleotides RPSI07_RS23560 and RPSI07_RS23565 are separated by 36 nucleotides RPSI07_RS23565 and RPSI07_RS23570 overlap by 4 nucleotides RPSI07_RS23570 and RPSI07_RS23575 are separated by 13 nucleotides
RPSI07_RS23555: RPSI07_RS23555 - tRNA-Phe, at 3,426,852 to 3,426,927
_RS23555
RPSI07_RS23560: RPSI07_RS23560 - two-component system response regulator, at 3,426,992 to 3,427,711
_RS23560
RPSI07_RS23565: RPSI07_RS23565 - PAS domain-containing sensor histidine kinase, at 3,427,748 to 3,430,183
_RS23565
RPSI07_RS23570: RPSI07_RS23570 - DUF4390 domain-containing protein, at 3,430,180 to 3,430,980
_RS23570
RPSI07_RS23575: RPSI07_RS23575 - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 3,430,994 to 3,432,319
_RS23575
Position (kb)
3427
3428
3429
3430
3431 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 3427.723 kb on - strand at 3427.723 kb on - strand at 3427.723 kb on - strand at 3427.723 kb on - strand at 3427.723 kb on - strand at 3427.750 kb on - strand at 3427.750 kb on - strand at 3427.761 kb on - strand at 3428.009 kb on + strand, within RPSI07_RS23565 at 3428.216 kb on + strand, within RPSI07_RS23565 at 3428.280 kb on + strand, within RPSI07_RS23565 at 3429.151 kb on - strand, within RPSI07_RS23565 at 3429.151 kb on - strand, within RPSI07_RS23565 at 3429.879 kb on + strand, within RPSI07_RS23565 at 3429.880 kb on - strand, within RPSI07_RS23565 at 3430.075 kb on + strand at 3430.164 kb on + strand at 3430.165 kb on - strand at 3430.165 kb on - strand at 3431.020 kb on - strand at 3431.023 kb on - strand at 3431.023 kb on - strand at 3431.124 kb on - strand at 3431.124 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 3,427,723 - +1.3 3,427,723 - +4.0 3,427,723 - +3.2 3,427,723 - +2.0 3,427,723 - +5.1 3,427,750 - +0.2 3,427,750 - +0.1 3,427,761 - +2.9 3,428,009 + RPSI07_RS23565 0.11 +2.8 3,428,216 + RPSI07_RS23565 0.19 +2.8 3,428,280 + RPSI07_RS23565 0.22 +4.9 3,429,151 - RPSI07_RS23565 0.58 +1.8 3,429,151 - RPSI07_RS23565 0.58 +2.0 3,429,879 + RPSI07_RS23565 0.87 +1.8 3,429,880 - RPSI07_RS23565 0.88 +1.8 3,430,075 + +1.8 3,430,164 + +0.6 3,430,165 - -0.6 3,430,165 - +2.1 3,431,020 - -0.7 3,431,023 - +0.8 3,431,023 - -0.4 3,431,124 - +1.5 3,431,124 - -0.6
Or see this region's nucleotide sequence