Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22505 and RPSI07_RS22510 are separated by 91 nucleotides RPSI07_RS22510 and RPSI07_RS22515 are separated by 94 nucleotides RPSI07_RS22515 and RPSI07_RS22520 are separated by 73 nucleotides
RPSI07_RS22505: RPSI07_RS22505 - hypothetical protein, at 3,204,069 to 3,204,638
_RS22505
RPSI07_RS22510: RPSI07_RS22510 - O-acetyl-ADP-ribose deacetylase, at 3,204,730 to 3,205,245
_RS22510
RPSI07_RS22515: RPSI07_RS22515 - membrane protein, at 3,205,340 to 3,205,894
_RS22515
RPSI07_RS22520: RPSI07_RS22520 - sigma-54-dependent Fis family transcriptional regulator, at 3,205,968 to 3,207,284
_RS22520
Position (kb)
3205
3206 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3204.508 kb on + strand, within RPSI07_RS22505 at 3204.509 kb on - strand at 3204.520 kb on + strand, within RPSI07_RS22505 at 3204.520 kb on + strand, within RPSI07_RS22505 at 3204.545 kb on + strand, within RPSI07_RS22505 at 3204.545 kb on + strand, within RPSI07_RS22505 at 3204.546 kb on - strand, within RPSI07_RS22505 at 3204.546 kb on - strand, within RPSI07_RS22505 at 3204.557 kb on - strand, within RPSI07_RS22505 at 3204.678 kb on + strand at 3204.678 kb on + strand at 3204.678 kb on + strand at 3204.678 kb on + strand at 3204.678 kb on + strand at 3204.679 kb on - strand at 3204.679 kb on - strand at 3204.679 kb on - strand at 3204.699 kb on + strand at 3204.700 kb on - strand at 3204.700 kb on - strand at 3204.700 kb on - strand at 3204.700 kb on - strand at 3204.741 kb on + strand at 3204.955 kb on + strand, within RPSI07_RS22510 at 3204.955 kb on + strand, within RPSI07_RS22510 at 3204.955 kb on + strand, within RPSI07_RS22510 at 3204.955 kb on + strand, within RPSI07_RS22510 at 3204.956 kb on - strand, within RPSI07_RS22510 at 3205.081 kb on - strand, within RPSI07_RS22510 at 3205.102 kb on + strand, within RPSI07_RS22510 at 3205.102 kb on + strand, within RPSI07_RS22510 at 3205.102 kb on + strand, within RPSI07_RS22510 at 3205.102 kb on + strand, within RPSI07_RS22510 at 3205.103 kb on - strand, within RPSI07_RS22510 at 3205.217 kb on - strand at 3205.217 kb on - strand at 3205.296 kb on + strand at 3205.297 kb on - strand at 3205.297 kb on - strand at 3205.297 kb on - strand at 3205.616 kb on + strand, within RPSI07_RS22515 at 3205.616 kb on + strand, within RPSI07_RS22515 at 3205.617 kb on - strand, within RPSI07_RS22515 at 3205.617 kb on - strand, within RPSI07_RS22515 at 3205.617 kb on - strand, within RPSI07_RS22515 at 3205.667 kb on + strand, within RPSI07_RS22515 at 3205.667 kb on + strand, within RPSI07_RS22515 at 3205.667 kb on + strand, within RPSI07_RS22515 at 3205.667 kb on + strand, within RPSI07_RS22515 at 3205.682 kb on + strand, within RPSI07_RS22515 at 3205.683 kb on - strand, within RPSI07_RS22515 at 3205.694 kb on + strand, within RPSI07_RS22515 at 3205.826 kb on + strand, within RPSI07_RS22515 at 3205.997 kb on - strand at 3206.090 kb on - strand at 3206.375 kb on - strand, within RPSI07_RS22520 at 3206.377 kb on + strand, within RPSI07_RS22520 at 3206.377 kb on + strand, within RPSI07_RS22520 at 3206.378 kb on - strand, within RPSI07_RS22520 at 3206.378 kb on - strand, within RPSI07_RS22520
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 3,204,508 + RPSI07_RS22505 0.77 +0.2 3,204,509 - +0.2 3,204,520 + RPSI07_RS22505 0.79 -1.3 3,204,520 + RPSI07_RS22505 0.79 -1.4 3,204,545 + RPSI07_RS22505 0.84 +1.2 3,204,545 + RPSI07_RS22505 0.84 +2.8 3,204,546 - RPSI07_RS22505 0.84 -0.5 3,204,546 - RPSI07_RS22505 0.84 +0.2 3,204,557 - RPSI07_RS22505 0.86 -0.6 3,204,678 + +0.5 3,204,678 + +0.1 3,204,678 + +0.2 3,204,678 + +0.8 3,204,678 + +1.0 3,204,679 - -1.0 3,204,679 - -0.8 3,204,679 - -1.0 3,204,699 + -0.2 3,204,700 - +0.2 3,204,700 - +0.8 3,204,700 - +0.1 3,204,700 - -0.8 3,204,741 + -1.9 3,204,955 + RPSI07_RS22510 0.44 +0.8 3,204,955 + RPSI07_RS22510 0.44 +0.3 3,204,955 + RPSI07_RS22510 0.44 -0.4 3,204,955 + RPSI07_RS22510 0.44 -2.8 3,204,956 - RPSI07_RS22510 0.44 +0.1 3,205,081 - RPSI07_RS22510 0.68 -0.0 3,205,102 + RPSI07_RS22510 0.72 +0.2 3,205,102 + RPSI07_RS22510 0.72 +0.5 3,205,102 + RPSI07_RS22510 0.72 -1.6 3,205,102 + RPSI07_RS22510 0.72 +0.3 3,205,103 - RPSI07_RS22510 0.72 -0.7 3,205,217 - +1.8 3,205,217 - -1.0 3,205,296 + -1.4 3,205,297 - +0.2 3,205,297 - -0.2 3,205,297 - +3.8 3,205,616 + RPSI07_RS22515 0.50 -1.0 3,205,616 + RPSI07_RS22515 0.50 +1.8 3,205,617 - RPSI07_RS22515 0.50 -0.2 3,205,617 - RPSI07_RS22515 0.50 -0.2 3,205,617 - RPSI07_RS22515 0.50 -1.0 3,205,667 + RPSI07_RS22515 0.59 -0.8 3,205,667 + RPSI07_RS22515 0.59 -0.8 3,205,667 + RPSI07_RS22515 0.59 -0.2 3,205,667 + RPSI07_RS22515 0.59 -1.7 3,205,682 + RPSI07_RS22515 0.62 -2.6 3,205,683 - RPSI07_RS22515 0.62 -1.2 3,205,694 + RPSI07_RS22515 0.64 +0.2 3,205,826 + RPSI07_RS22515 0.88 -1.0 3,205,997 - +2.2 3,206,090 - -0.2 3,206,375 - RPSI07_RS22520 0.31 -2.0 3,206,377 + RPSI07_RS22520 0.31 -0.8 3,206,377 + RPSI07_RS22520 0.31 +1.7 3,206,378 - RPSI07_RS22520 0.31 +0.6 3,206,378 - RPSI07_RS22520 0.31 -0.4
Or see this region's nucleotide sequence