Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS22225

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS22215 and RPSI07_RS22220 are separated by 75 nucleotidesRPSI07_RS22220 and RPSI07_RS22225 are separated by 50 nucleotidesRPSI07_RS22225 and RPSI07_RS22230 are separated by 195 nucleotidesRPSI07_RS22230 and RPSI07_RS22235 are separated by 79 nucleotides RPSI07_RS22215: RPSI07_RS22215 - DUF4124 domain-containing protein, at 3,150,799 to 3,151,461 _RS22215 RPSI07_RS22220: RPSI07_RS22220 - 4Fe-4S dicluster domain-containing protein, at 3,151,537 to 3,151,785 _RS22220 RPSI07_RS22225: RPSI07_RS22225 - phosphopantetheine adenylyltransferase, at 3,151,836 to 3,152,342 _RS22225 RPSI07_RS22230: RPSI07_RS22230 - 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD, at 3,152,538 to 3,153,185 _RS22230 RPSI07_RS22235: RPSI07_RS22235 - insulinase family protein, at 3,153,265 to 3,154,608 _RS22235 Position (kb) 3151 3152 3153Strain fitness (log2 ratio) -2 -1 0 1 2at 3150.898 kb on + strand, within RPSI07_RS22215at 3150.929 kb on - strand, within RPSI07_RS22215at 3151.198 kb on + strand, within RPSI07_RS22215at 3151.199 kb on - strand, within RPSI07_RS22215at 3151.216 kb on + strand, within RPSI07_RS22215at 3151.216 kb on + strand, within RPSI07_RS22215at 3151.422 kb on + strandat 3151.422 kb on + strandat 3151.422 kb on + strandat 3151.422 kb on + strandat 3151.423 kb on - strandat 3151.423 kb on - strandat 3151.423 kb on - strandat 3151.423 kb on - strandat 3151.423 kb on - strandat 3151.423 kb on - strandat 3151.426 kb on + strandat 3151.427 kb on - strandat 3151.427 kb on - strandat 3151.427 kb on - strandat 3151.560 kb on - strandat 3151.560 kb on - strandat 3151.837 kb on + strandat 3152.535 kb on - strandat 3152.535 kb on - strandat 3152.690 kb on - strand, within RPSI07_RS22230at 3152.690 kb on - strand, within RPSI07_RS22230at 3153.086 kb on + strand, within RPSI07_RS22230at 3153.087 kb on - strand, within RPSI07_RS22230at 3153.087 kb on - strand, within RPSI07_RS22230at 3153.094 kb on + strand, within RPSI07_RS22230at 3153.094 kb on + strand, within RPSI07_RS22230at 3153.095 kb on - strand, within RPSI07_RS22230at 3153.095 kb on - strand, within RPSI07_RS22230at 3153.197 kb on + strandat 3153.197 kb on + strandat 3153.197 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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3,150,898 + RPSI07_RS22215 0.15 -2.0
3,150,929 - RPSI07_RS22215 0.20 -0.3
3,151,198 + RPSI07_RS22215 0.60 -1.0
3,151,199 - RPSI07_RS22215 0.60 +1.2
3,151,216 + RPSI07_RS22215 0.63 -0.8
3,151,216 + RPSI07_RS22215 0.63 -0.3
3,151,422 + +1.1
3,151,422 + +0.3
3,151,422 + +1.0
3,151,422 + -1.0
3,151,423 - +0.1
3,151,423 - +1.7
3,151,423 - +0.2
3,151,423 - +0.2
3,151,423 - -1.1
3,151,423 - +0.7
3,151,426 + +0.3
3,151,427 - -0.5
3,151,427 - -1.0
3,151,427 - -2.0
3,151,560 - -0.5
3,151,560 - +0.5
3,151,837 + -0.1
3,152,535 - -0.3
3,152,535 - -0.6
3,152,690 - RPSI07_RS22230 0.23 +0.8
3,152,690 - RPSI07_RS22230 0.23 +1.7
3,153,086 + RPSI07_RS22230 0.85 -0.0
3,153,087 - RPSI07_RS22230 0.85 +1.3
3,153,087 - RPSI07_RS22230 0.85 +1.7
3,153,094 + RPSI07_RS22230 0.86 +1.1
3,153,094 + RPSI07_RS22230 0.86 +1.3
3,153,095 - RPSI07_RS22230 0.86 +2.3
3,153,095 - RPSI07_RS22230 0.86 -2.3
3,153,197 + +0.4
3,153,197 + +2.1
3,153,197 + -0.7

Or see this region's nucleotide sequence