Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22215 and RPSI07_RS22220 are separated by 75 nucleotides RPSI07_RS22220 and RPSI07_RS22225 are separated by 50 nucleotides RPSI07_RS22225 and RPSI07_RS22230 are separated by 195 nucleotides RPSI07_RS22230 and RPSI07_RS22235 are separated by 79 nucleotides
RPSI07_RS22215: RPSI07_RS22215 - DUF4124 domain-containing protein, at 3,150,799 to 3,151,461
_RS22215
RPSI07_RS22220: RPSI07_RS22220 - 4Fe-4S dicluster domain-containing protein, at 3,151,537 to 3,151,785
_RS22220
RPSI07_RS22225: RPSI07_RS22225 - phosphopantetheine adenylyltransferase, at 3,151,836 to 3,152,342
_RS22225
RPSI07_RS22230: RPSI07_RS22230 - 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD, at 3,152,538 to 3,153,185
_RS22230
RPSI07_RS22235: RPSI07_RS22235 - insulinase family protein, at 3,153,265 to 3,154,608
_RS22235
Position (kb)
3151
3152
3153 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3150.898 kb on + strand, within RPSI07_RS22215 at 3150.929 kb on - strand, within RPSI07_RS22215 at 3151.198 kb on + strand, within RPSI07_RS22215 at 3151.199 kb on - strand, within RPSI07_RS22215 at 3151.216 kb on + strand, within RPSI07_RS22215 at 3151.216 kb on + strand, within RPSI07_RS22215 at 3151.422 kb on + strand at 3151.422 kb on + strand at 3151.422 kb on + strand at 3151.422 kb on + strand at 3151.423 kb on - strand at 3151.423 kb on - strand at 3151.423 kb on - strand at 3151.423 kb on - strand at 3151.423 kb on - strand at 3151.423 kb on - strand at 3151.426 kb on + strand at 3151.427 kb on - strand at 3151.427 kb on - strand at 3151.427 kb on - strand at 3151.560 kb on - strand at 3151.560 kb on - strand at 3151.837 kb on + strand at 3152.535 kb on - strand at 3152.535 kb on - strand at 3152.690 kb on - strand, within RPSI07_RS22230 at 3152.690 kb on - strand, within RPSI07_RS22230 at 3153.086 kb on + strand, within RPSI07_RS22230 at 3153.087 kb on - strand, within RPSI07_RS22230 at 3153.087 kb on - strand, within RPSI07_RS22230 at 3153.094 kb on + strand, within RPSI07_RS22230 at 3153.094 kb on + strand, within RPSI07_RS22230 at 3153.095 kb on - strand, within RPSI07_RS22230 at 3153.095 kb on - strand, within RPSI07_RS22230 at 3153.197 kb on + strand at 3153.197 kb on + strand at 3153.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 3,150,898 + RPSI07_RS22215 0.15 -2.0 3,150,929 - RPSI07_RS22215 0.20 -0.3 3,151,198 + RPSI07_RS22215 0.60 -1.0 3,151,199 - RPSI07_RS22215 0.60 +1.2 3,151,216 + RPSI07_RS22215 0.63 -0.8 3,151,216 + RPSI07_RS22215 0.63 -0.3 3,151,422 + +1.1 3,151,422 + +0.3 3,151,422 + +1.0 3,151,422 + -1.0 3,151,423 - +0.1 3,151,423 - +1.7 3,151,423 - +0.2 3,151,423 - +0.2 3,151,423 - -1.1 3,151,423 - +0.7 3,151,426 + +0.3 3,151,427 - -0.5 3,151,427 - -1.0 3,151,427 - -2.0 3,151,560 - -0.5 3,151,560 - +0.5 3,151,837 + -0.1 3,152,535 - -0.3 3,152,535 - -0.6 3,152,690 - RPSI07_RS22230 0.23 +0.8 3,152,690 - RPSI07_RS22230 0.23 +1.7 3,153,086 + RPSI07_RS22230 0.85 -0.0 3,153,087 - RPSI07_RS22230 0.85 +1.3 3,153,087 - RPSI07_RS22230 0.85 +1.7 3,153,094 + RPSI07_RS22230 0.86 +1.1 3,153,094 + RPSI07_RS22230 0.86 +1.3 3,153,095 - RPSI07_RS22230 0.86 +2.3 3,153,095 - RPSI07_RS22230 0.86 -2.3 3,153,197 + +0.4 3,153,197 + +2.1 3,153,197 + -0.7
Or see this region's nucleotide sequence