Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22145 and RPSI07_RS22150 are separated by 191 nucleotides RPSI07_RS22150 and RPSI07_RS22155 are separated by 92 nucleotides RPSI07_RS22155 and RPSI07_RS22160 are separated by 79 nucleotides RPSI07_RS22160 and RPSI07_RS22165 are separated by 18 nucleotides
RPSI07_RS22145: RPSI07_RS22145 - HPr kinase/phosphorylase, at 3,136,979 to 3,137,953
_RS22145
RPSI07_RS22150: RPSI07_RS22150 - hypothetical protein, at 3,138,145 to 3,138,474
_RS22150
RPSI07_RS22155: RPSI07_RS22155 - RNase adapter RapZ, at 3,138,567 to 3,139,457
_RS22155
RPSI07_RS22160: RPSI07_RS22160 - ATP-dependent protease, at 3,139,537 to 3,140,187
_RS22160
RPSI07_RS22165: RPSI07_RS22165 - A/G-specific adenine glycosylase, at 3,140,206 to 3,141,354
_RS22165
Position (kb)
3138
3139
3140 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3137.952 kb on - strand at 3137.952 kb on - strand at 3138.302 kb on - strand, within RPSI07_RS22150 at 3138.302 kb on - strand, within RPSI07_RS22150 at 3138.302 kb on - strand, within RPSI07_RS22150 at 3138.302 kb on - strand, within RPSI07_RS22150 at 3138.302 kb on - strand, within RPSI07_RS22150 at 3138.302 kb on - strand, within RPSI07_RS22150 at 3138.302 kb on - strand, within RPSI07_RS22150 at 3138.302 kb on - strand, within RPSI07_RS22150 at 3138.334 kb on - strand, within RPSI07_RS22150 at 3138.434 kb on - strand, within RPSI07_RS22150 at 3138.484 kb on + strand at 3138.554 kb on + strand at 3138.555 kb on + strand at 3138.643 kb on - strand at 3138.643 kb on - strand at 3138.645 kb on + strand at 3138.645 kb on + strand at 3138.646 kb on - strand at 3139.049 kb on + strand, within RPSI07_RS22155 at 3139.051 kb on - strand, within RPSI07_RS22155 at 3139.177 kb on - strand, within RPSI07_RS22155 at 3139.179 kb on + strand, within RPSI07_RS22155 at 3139.179 kb on + strand, within RPSI07_RS22155 at 3139.179 kb on + strand, within RPSI07_RS22155 at 3139.185 kb on - strand, within RPSI07_RS22155 at 3139.281 kb on + strand, within RPSI07_RS22155 at 3139.282 kb on - strand, within RPSI07_RS22155 at 3139.326 kb on + strand, within RPSI07_RS22155 at 3139.327 kb on - strand, within RPSI07_RS22155 at 3139.374 kb on + strand at 3139.375 kb on - strand at 3139.375 kb on - strand at 3139.376 kb on + strand at 3139.376 kb on + strand at 3139.416 kb on - strand at 3139.416 kb on - strand at 3139.416 kb on - strand at 3139.548 kb on + strand at 3139.549 kb on - strand at 3139.549 kb on - strand at 3139.667 kb on - strand, within RPSI07_RS22160 at 3139.667 kb on - strand, within RPSI07_RS22160 at 3139.895 kb on + strand, within RPSI07_RS22160 at 3140.035 kb on + strand, within RPSI07_RS22160 at 3140.035 kb on + strand, within RPSI07_RS22160 at 3140.161 kb on + strand at 3140.162 kb on - strand at 3140.186 kb on - strand at 3140.186 kb on - strand at 3140.376 kb on + strand, within RPSI07_RS22165 at 3140.420 kb on + strand, within RPSI07_RS22165 at 3140.420 kb on + strand, within RPSI07_RS22165 at 3140.421 kb on - strand, within RPSI07_RS22165
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 3,137,952 - -0.1 3,137,952 - +0.0 3,138,302 - RPSI07_RS22150 0.48 +0.6 3,138,302 - RPSI07_RS22150 0.48 +1.4 3,138,302 - RPSI07_RS22150 0.48 +1.9 3,138,302 - RPSI07_RS22150 0.48 -0.5 3,138,302 - RPSI07_RS22150 0.48 -1.4 3,138,302 - RPSI07_RS22150 0.48 +1.1 3,138,302 - RPSI07_RS22150 0.48 +0.4 3,138,302 - RPSI07_RS22150 0.48 +1.1 3,138,334 - RPSI07_RS22150 0.57 +0.6 3,138,434 - RPSI07_RS22150 0.88 -1.5 3,138,484 + -0.6 3,138,554 + -1.0 3,138,555 + -2.6 3,138,643 - +0.1 3,138,643 - -1.1 3,138,645 + -1.3 3,138,645 + -1.1 3,138,646 - +0.7 3,139,049 + RPSI07_RS22155 0.54 -2.4 3,139,051 - RPSI07_RS22155 0.54 -0.6 3,139,177 - RPSI07_RS22155 0.68 +2.3 3,139,179 + RPSI07_RS22155 0.69 -0.3 3,139,179 + RPSI07_RS22155 0.69 -1.9 3,139,179 + RPSI07_RS22155 0.69 -0.3 3,139,185 - RPSI07_RS22155 0.69 +1.7 3,139,281 + RPSI07_RS22155 0.80 -1.6 3,139,282 - RPSI07_RS22155 0.80 +0.7 3,139,326 + RPSI07_RS22155 0.85 -0.9 3,139,327 - RPSI07_RS22155 0.85 -1.6 3,139,374 + -1.6 3,139,375 - -0.6 3,139,375 - +0.4 3,139,376 + -1.6 3,139,376 + +0.7 3,139,416 - +1.7 3,139,416 - +0.7 3,139,416 - -0.9 3,139,548 + -1.1 3,139,549 - -1.6 3,139,549 - +0.5 3,139,667 - RPSI07_RS22160 0.20 -2.2 3,139,667 - RPSI07_RS22160 0.20 +1.1 3,139,895 + RPSI07_RS22160 0.55 +1.7 3,140,035 + RPSI07_RS22160 0.76 -2.6 3,140,035 + RPSI07_RS22160 0.76 +0.8 3,140,161 + -0.6 3,140,162 - +0.2 3,140,186 - -1.2 3,140,186 - -0.7 3,140,376 + RPSI07_RS22165 0.15 -1.3 3,140,420 + RPSI07_RS22165 0.19 -0.3 3,140,420 + RPSI07_RS22165 0.19 +0.5 3,140,421 - RPSI07_RS22165 0.19 -2.3
Or see this region's nucleotide sequence