Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS18495

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS18485 and RPSI07_RS18490 are separated by 57 nucleotidesRPSI07_RS18490 and RPSI07_RS18495 are separated by 22 nucleotidesRPSI07_RS18495 and RPSI07_RS18500 are separated by 150 nucleotides RPSI07_RS18485: RPSI07_RS18485 - site-2 protease family protein, at 2,349,338 to 2,350,015 _RS18485 RPSI07_RS18490: RPSI07_RS18490 - threonylcarbamoyl-AMP synthase, at 2,350,073 to 2,350,696 _RS18490 RPSI07_RS18495: RPSI07_RS18495 - PHP domain-containing protein, at 2,350,719 to 2,351,579 _RS18495 RPSI07_RS18500: RPSI07_RS18500 - NAD(P)/FAD-dependent oxidoreductase, at 2,351,730 to 2,352,809 _RS18500 Position (kb) 2350 2351 2352Strain fitness (log2 ratio) -1 0 1 2 3 4at 2349.795 kb on - strand, within RPSI07_RS18485at 2349.820 kb on - strand, within RPSI07_RS18485at 2349.820 kb on - strand, within RPSI07_RS18485at 2349.820 kb on - strand, within RPSI07_RS18485at 2349.820 kb on - strand, within RPSI07_RS18485at 2349.931 kb on - strand, within RPSI07_RS18485at 2350.029 kb on + strandat 2350.029 kb on + strandat 2350.029 kb on + strandat 2350.030 kb on - strandat 2350.030 kb on - strandat 2350.075 kb on - strandat 2350.075 kb on - strandat 2350.075 kb on - strandat 2350.075 kb on - strandat 2350.584 kb on + strand, within RPSI07_RS18490at 2350.861 kb on + strand, within RPSI07_RS18495at 2350.862 kb on - strand, within RPSI07_RS18495at 2350.865 kb on - strand, within RPSI07_RS18495at 2350.870 kb on + strand, within RPSI07_RS18495at 2350.973 kb on - strand, within RPSI07_RS18495at 2351.200 kb on + strand, within RPSI07_RS18495at 2351.200 kb on + strand, within RPSI07_RS18495at 2351.201 kb on - strand, within RPSI07_RS18495at 2351.380 kb on + strand, within RPSI07_RS18495at 2351.380 kb on + strand, within RPSI07_RS18495at 2351.381 kb on - strand, within RPSI07_RS18495at 2351.381 kb on - strand, within RPSI07_RS18495at 2351.381 kb on - strand, within RPSI07_RS18495at 2351.590 kb on + strandat 2351.590 kb on + strandat 2351.696 kb on - strandat 2351.696 kb on - strandat 2351.928 kb on + strand, within RPSI07_RS18500at 2351.928 kb on + strand, within RPSI07_RS18500at 2351.928 kb on + strand, within RPSI07_RS18500at 2351.929 kb on - strand, within RPSI07_RS18500at 2352.118 kb on + strand, within RPSI07_RS18500at 2352.204 kb on + strand, within RPSI07_RS18500at 2352.204 kb on + strand, within RPSI07_RS18500at 2352.204 kb on + strand, within RPSI07_RS18500at 2352.205 kb on - strand, within RPSI07_RS18500

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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2,349,795 - RPSI07_RS18485 0.67 +0.5
2,349,820 - RPSI07_RS18485 0.71 +0.2
2,349,820 - RPSI07_RS18485 0.71 +0.6
2,349,820 - RPSI07_RS18485 0.71 +0.0
2,349,820 - RPSI07_RS18485 0.71 -0.2
2,349,931 - RPSI07_RS18485 0.87 +0.0
2,350,029 + -0.3
2,350,029 + -1.3
2,350,029 + -0.3
2,350,030 - +0.1
2,350,030 - +0.4
2,350,075 - -0.6
2,350,075 - -1.4
2,350,075 - +0.0
2,350,075 - +0.6
2,350,584 + RPSI07_RS18490 0.82 +0.3
2,350,861 + RPSI07_RS18495 0.16 +0.9
2,350,862 - RPSI07_RS18495 0.17 -0.3
2,350,865 - RPSI07_RS18495 0.17 -0.5
2,350,870 + RPSI07_RS18495 0.18 -0.7
2,350,973 - RPSI07_RS18495 0.30 -0.6
2,351,200 + RPSI07_RS18495 0.56 -1.1
2,351,200 + RPSI07_RS18495 0.56 +0.0
2,351,201 - RPSI07_RS18495 0.56 -1.6
2,351,380 + RPSI07_RS18495 0.77 +0.6
2,351,380 + RPSI07_RS18495 0.77 -0.9
2,351,381 - RPSI07_RS18495 0.77 -1.2
2,351,381 - RPSI07_RS18495 0.77 -0.2
2,351,381 - RPSI07_RS18495 0.77 +1.8
2,351,590 + -0.2
2,351,590 + -0.4
2,351,696 - +1.1
2,351,696 - +0.1
2,351,928 + RPSI07_RS18500 0.18 +0.6
2,351,928 + RPSI07_RS18500 0.18 +3.9
2,351,928 + RPSI07_RS18500 0.18 -0.2
2,351,929 - RPSI07_RS18500 0.18 +0.0
2,352,118 + RPSI07_RS18500 0.36 +0.3
2,352,204 + RPSI07_RS18500 0.44 +0.8
2,352,204 + RPSI07_RS18500 0.44 -0.2
2,352,204 + RPSI07_RS18500 0.44 +0.4
2,352,205 - RPSI07_RS18500 0.44 +1.2

Or see this region's nucleotide sequence