Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS18470 and RPSI07_RS18475 are separated by 119 nucleotides RPSI07_RS18475 and RPSI07_RS18480 are separated by 20 nucleotides RPSI07_RS18480 and RPSI07_RS18485 are separated by 13 nucleotides RPSI07_RS18485 and RPSI07_RS18490 are separated by 57 nucleotides
RPSI07_RS18470: RPSI07_RS18470 - 4-hydroxy-tetrahydrodipicolinate synthase, at 2,346,531 to 2,347,415
_RS18470
RPSI07_RS18475: RPSI07_RS18475 - class I SAM-dependent methyltransferase, at 2,347,535 to 2,348,101
_RS18475
RPSI07_RS18480: RPSI07_RS18480 - tryptophan--tRNA ligase, at 2,348,122 to 2,349,324
_RS18480
RPSI07_RS18485: RPSI07_RS18485 - site-2 protease family protein, at 2,349,338 to 2,350,015
_RS18485
RPSI07_RS18490: RPSI07_RS18490 - threonylcarbamoyl-AMP synthase, at 2,350,073 to 2,350,696
_RS18490
Position (kb)
2348
2349
2350 Strain fitness (log2 ratio)
-1
0
1
2 at 2347.446 kb on - strand at 2347.461 kb on + strand at 2347.599 kb on + strand, within RPSI07_RS18475 at 2347.600 kb on - strand, within RPSI07_RS18475 at 2347.812 kb on + strand, within RPSI07_RS18475 at 2347.812 kb on + strand, within RPSI07_RS18475 at 2347.813 kb on - strand, within RPSI07_RS18475 at 2349.340 kb on - strand at 2349.472 kb on - strand, within RPSI07_RS18485 at 2349.472 kb on - strand, within RPSI07_RS18485 at 2349.472 kb on - strand, within RPSI07_RS18485 at 2349.795 kb on - strand, within RPSI07_RS18485 at 2349.820 kb on - strand, within RPSI07_RS18485 at 2349.820 kb on - strand, within RPSI07_RS18485 at 2349.820 kb on - strand, within RPSI07_RS18485 at 2349.820 kb on - strand, within RPSI07_RS18485 at 2349.931 kb on - strand, within RPSI07_RS18485 at 2350.029 kb on + strand at 2350.029 kb on + strand at 2350.029 kb on + strand at 2350.030 kb on - strand at 2350.030 kb on - strand at 2350.075 kb on - strand at 2350.075 kb on - strand at 2350.075 kb on - strand at 2350.075 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 2,347,446 - -0.8 2,347,461 + +1.6 2,347,599 + RPSI07_RS18475 0.11 +0.6 2,347,600 - RPSI07_RS18475 0.11 -1.2 2,347,812 + RPSI07_RS18475 0.49 +1.0 2,347,812 + RPSI07_RS18475 0.49 -1.5 2,347,813 - RPSI07_RS18475 0.49 -0.4 2,349,340 - +0.3 2,349,472 - RPSI07_RS18485 0.20 +2.6 2,349,472 - RPSI07_RS18485 0.20 +0.3 2,349,472 - RPSI07_RS18485 0.20 -0.1 2,349,795 - RPSI07_RS18485 0.67 +0.5 2,349,820 - RPSI07_RS18485 0.71 +0.2 2,349,820 - RPSI07_RS18485 0.71 +0.6 2,349,820 - RPSI07_RS18485 0.71 +0.0 2,349,820 - RPSI07_RS18485 0.71 -0.2 2,349,931 - RPSI07_RS18485 0.87 +0.0 2,350,029 + -0.3 2,350,029 + -1.3 2,350,029 + -0.3 2,350,030 - +0.1 2,350,030 - +0.4 2,350,075 - -0.6 2,350,075 - -1.4 2,350,075 - +0.0 2,350,075 - +0.6
Or see this region's nucleotide sequence