Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10705 and RPSI07_RS10710 are separated by 7 nucleotides RPSI07_RS10710 and RPSI07_RS10715 are separated by 65 nucleotides RPSI07_RS10715 and RPSI07_RS10720 overlap by 4 nucleotides RPSI07_RS10720 and RPSI07_RS10725 are separated by 92 nucleotides
RPSI07_RS10705: RPSI07_RS10705 - FUSC family protein, at 603,618 to 605,831
_RS10705
RPSI07_RS10710: RPSI07_RS10710 - hypothetical protein, at 605,839 to 606,075
_RS10710
RPSI07_RS10715: RPSI07_RS10715 - hypothetical protein, at 606,141 to 606,437
_RS10715
RPSI07_RS10720: RPSI07_RS10720 - hypothetical protein, at 606,434 to 606,891
_RS10720
RPSI07_RS10725: RPSI07_RS10725 - uracil-DNA glycosylase, at 606,984 to 607,778
_RS10725
Position (kb) 606
607 Strain fitness (log2 ratio) -2
-1
0
1
2 at 605.159 kb on + strand, within RPSI07_RS10705 at 605.197 kb on - strand, within RPSI07_RS10705 at 605.197 kb on - strand, within RPSI07_RS10705 at 605.197 kb on - strand, within RPSI07_RS10705 at 605.217 kb on + strand, within RPSI07_RS10705 at 605.217 kb on + strand, within RPSI07_RS10705 at 605.451 kb on + strand, within RPSI07_RS10705 at 605.451 kb on + strand, within RPSI07_RS10705 at 605.452 kb on - strand, within RPSI07_RS10705 at 605.978 kb on + strand, within RPSI07_RS10710 at 606.107 kb on - strand at 606.134 kb on - strand at 606.220 kb on - strand, within RPSI07_RS10715 at 606.419 kb on + strand at 606.456 kb on + strand at 606.523 kb on - strand, within RPSI07_RS10720 at 606.523 kb on - strand, within RPSI07_RS10720 at 606.638 kb on - strand, within RPSI07_RS10720 at 606.654 kb on + strand, within RPSI07_RS10720 at 606.734 kb on + strand, within RPSI07_RS10720 at 606.734 kb on + strand, within RPSI07_RS10720 at 606.734 kb on + strand, within RPSI07_RS10720 at 606.737 kb on + strand, within RPSI07_RS10720 at 606.737 kb on + strand, within RPSI07_RS10720 at 606.737 kb on + strand, within RPSI07_RS10720 at 606.737 kb on + strand, within RPSI07_RS10720 at 606.737 kb on + strand, within RPSI07_RS10720 at 606.738 kb on - strand, within RPSI07_RS10720 at 606.738 kb on - strand, within RPSI07_RS10720 at 606.738 kb on - strand, within RPSI07_RS10720 at 606.738 kb on - strand, within RPSI07_RS10720 at 606.777 kb on + strand, within RPSI07_RS10720 at 606.777 kb on + strand, within RPSI07_RS10720 at 606.778 kb on - strand, within RPSI07_RS10720 at 606.778 kb on - strand, within RPSI07_RS10720 at 606.893 kb on + strand at 606.893 kb on + strand at 606.894 kb on - strand at 606.894 kb on - strand at 606.894 kb on - strand at 606.931 kb on + strand at 606.931 kb on + strand at 606.931 kb on + strand at 606.931 kb on + strand at 606.931 kb on + strand at 606.931 kb on + strand at 606.931 kb on + strand at 606.931 kb on + strand at 606.931 kb on + strand at 606.931 kb on + strand at 606.932 kb on - strand at 606.932 kb on - strand at 606.932 kb on - strand at 606.938 kb on + strand at 606.938 kb on + strand at 606.938 kb on + strand at 606.939 kb on - strand at 606.939 kb on - strand at 606.939 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 605,159 + RPSI07_RS10705 0.70 +0.2 605,197 - RPSI07_RS10705 0.71 -1.2 605,197 - RPSI07_RS10705 0.71 +0.4 605,197 - RPSI07_RS10705 0.71 -0.3 605,217 + RPSI07_RS10705 0.72 -1.0 605,217 + RPSI07_RS10705 0.72 -1.7 605,451 + RPSI07_RS10705 0.83 -1.0 605,451 + RPSI07_RS10705 0.83 -0.3 605,452 - RPSI07_RS10705 0.83 -0.1 605,978 + RPSI07_RS10710 0.59 +1.3 606,107 - -1.7 606,134 - -0.5 606,220 - RPSI07_RS10715 0.27 +2.7 606,419 + +0.9 606,456 + -0.3 606,523 - RPSI07_RS10720 0.19 +1.7 606,523 - RPSI07_RS10720 0.19 -1.2 606,638 - RPSI07_RS10720 0.45 -0.9 606,654 + RPSI07_RS10720 0.48 -0.8 606,734 + RPSI07_RS10720 0.66 +0.2 606,734 + RPSI07_RS10720 0.66 +0.1 606,734 + RPSI07_RS10720 0.66 -0.3 606,737 + RPSI07_RS10720 0.66 +0.8 606,737 + RPSI07_RS10720 0.66 +0.5 606,737 + RPSI07_RS10720 0.66 +1.5 606,737 + RPSI07_RS10720 0.66 -0.5 606,737 + RPSI07_RS10720 0.66 +0.3 606,738 - RPSI07_RS10720 0.66 -1.1 606,738 - RPSI07_RS10720 0.66 +2.7 606,738 - RPSI07_RS10720 0.66 -0.6 606,738 - RPSI07_RS10720 0.66 +0.1 606,777 + RPSI07_RS10720 0.75 +0.1 606,777 + RPSI07_RS10720 0.75 +1.7 606,778 - RPSI07_RS10720 0.75 -0.0 606,778 - RPSI07_RS10720 0.75 -1.3 606,893 + +0.3 606,893 + -2.0 606,894 - +1.7 606,894 - -0.7 606,894 - -0.7 606,931 + +0.4 606,931 + +0.1 606,931 + -1.0 606,931 + -1.1 606,931 + -0.1 606,931 + +0.7 606,931 + +0.3 606,931 + +0.3 606,931 + -0.0 606,931 + -0.0 606,932 - +0.2 606,932 - -0.5 606,932 - -2.2 606,938 + -0.1 606,938 + -1.5 606,938 + +0.4 606,939 - +1.8 606,939 - +0.7 606,939 - +1.7
Or see this region's nucleotide sequence