Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS10540

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS10535 and RPSI07_RS10540 are separated by 13 nucleotidesRPSI07_RS10540 and RPSI07_RS10545 are separated by 531 nucleotides RPSI07_RS10535: RPSI07_RS10535 - sensor histidine kinase, at 563,514 to 564,812 _RS10535 RPSI07_RS10540: RPSI07_RS10540 - TonB-denpendent receptor, at 564,826 to 566,925 _RS10540 RPSI07_RS10545: RPSI07_RS10545 - gamma-glutamyltransferase family protein, at 567,457 to 569,325 _RS10545 Position (kb) 564 565 566 567Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 564.577 kb on - strand, within RPSI07_RS10535at 564.769 kb on - strandat 564.769 kb on - strandat 564.769 kb on - strandat 564.769 kb on - strandat 564.953 kb on + strandat 564.954 kb on - strandat 565.034 kb on + strandat 565.075 kb on + strand, within RPSI07_RS10540at 565.075 kb on + strand, within RPSI07_RS10540at 565.075 kb on + strand, within RPSI07_RS10540at 565.076 kb on - strand, within RPSI07_RS10540at 565.076 kb on - strand, within RPSI07_RS10540at 565.076 kb on - strand, within RPSI07_RS10540at 565.076 kb on - strand, within RPSI07_RS10540at 565.076 kb on - strand, within RPSI07_RS10540at 565.113 kb on - strand, within RPSI07_RS10540at 565.196 kb on + strand, within RPSI07_RS10540at 565.196 kb on + strandat 565.196 kb on + strand, within RPSI07_RS10540at 565.197 kb on - strand, within RPSI07_RS10540at 565.197 kb on - strand, within RPSI07_RS10540at 565.197 kb on - strand, within RPSI07_RS10540at 565.280 kb on + strand, within RPSI07_RS10540at 565.280 kb on + strand, within RPSI07_RS10540at 565.280 kb on + strand, within RPSI07_RS10540at 565.281 kb on - strand, within RPSI07_RS10540at 565.462 kb on + strand, within RPSI07_RS10540at 565.463 kb on - strand, within RPSI07_RS10540at 565.614 kb on + strand, within RPSI07_RS10540at 565.614 kb on + strand, within RPSI07_RS10540at 565.614 kb on + strand, within RPSI07_RS10540at 565.615 kb on - strand, within RPSI07_RS10540at 565.615 kb on - strand, within RPSI07_RS10540at 565.736 kb on + strand, within RPSI07_RS10540at 565.737 kb on - strand, within RPSI07_RS10540at 565.784 kb on + strand, within RPSI07_RS10540at 565.976 kb on + strand, within RPSI07_RS10540at 565.976 kb on + strand, within RPSI07_RS10540at 565.976 kb on + strand, within RPSI07_RS10540at 565.977 kb on - strand, within RPSI07_RS10540at 565.977 kb on - strand, within RPSI07_RS10540at 565.977 kb on - strand, within RPSI07_RS10540at 565.977 kb on - strand, within RPSI07_RS10540at 566.018 kb on + strand, within RPSI07_RS10540at 566.019 kb on - strand, within RPSI07_RS10540at 566.078 kb on + strand, within RPSI07_RS10540at 566.078 kb on + strand, within RPSI07_RS10540at 566.238 kb on + strand, within RPSI07_RS10540at 566.238 kb on + strand, within RPSI07_RS10540at 566.239 kb on - strand, within RPSI07_RS10540at 566.319 kb on - strand, within RPSI07_RS10540at 566.378 kb on + strand, within RPSI07_RS10540at 566.426 kb on + strand, within RPSI07_RS10540at 566.427 kb on - strand, within RPSI07_RS10540at 566.427 kb on - strand, within RPSI07_RS10540at 566.427 kb on - strand, within RPSI07_RS10540at 566.477 kb on + strand, within RPSI07_RS10540at 566.477 kb on + strand, within RPSI07_RS10540at 566.478 kb on - strand, within RPSI07_RS10540at 566.478 kb on - strand, within RPSI07_RS10540at 566.492 kb on + strand, within RPSI07_RS10540at 566.492 kb on + strand, within RPSI07_RS10540at 566.492 kb on + strand, within RPSI07_RS10540at 566.541 kb on - strand, within RPSI07_RS10540at 566.541 kb on - strand, within RPSI07_RS10540at 566.639 kb on + strand, within RPSI07_RS10540at 566.640 kb on - strand, within RPSI07_RS10540at 566.753 kb on + strandat 566.753 kb on + strandat 566.754 kb on - strandat 566.967 kb on + strandat 566.967 kb on + strandat 566.967 kb on + strandat 567.065 kb on + strandat 567.065 kb on + strandat 567.065 kb on + strandat 567.065 kb on + strandat 567.065 kb on + strandat 567.066 kb on - strandat 567.066 kb on - strandat 567.066 kb on - strandat 567.066 kb on - strandat 567.761 kb on + strand, within RPSI07_RS10545at 567.818 kb on - strand, within RPSI07_RS10545at 567.818 kb on - strand, within RPSI07_RS10545at 567.845 kb on - strand, within RPSI07_RS10545

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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564,577 - RPSI07_RS10535 0.82 -0.1
564,769 - -0.0
564,769 - -0.3
564,769 - +0.2
564,769 - -0.5
564,953 + +0.2
564,954 - -0.3
565,034 + +0.0
565,075 + RPSI07_RS10540 0.12 -0.1
565,075 + RPSI07_RS10540 0.12 -0.1
565,075 + RPSI07_RS10540 0.12 -1.6
565,076 - RPSI07_RS10540 0.12 +0.7
565,076 - RPSI07_RS10540 0.12 -1.3
565,076 - RPSI07_RS10540 0.12 +3.7
565,076 - RPSI07_RS10540 0.12 +1.7
565,076 - RPSI07_RS10540 0.12 +0.9
565,113 - RPSI07_RS10540 0.14 +0.7
565,196 + RPSI07_RS10540 0.18 +0.2
565,196 + +3.3
565,196 + RPSI07_RS10540 0.18 +0.4
565,197 - RPSI07_RS10540 0.18 +0.3
565,197 - RPSI07_RS10540 0.18 -1.4
565,197 - RPSI07_RS10540 0.18 -1.6
565,280 + RPSI07_RS10540 0.22 -2.4
565,280 + RPSI07_RS10540 0.22 +1.0
565,280 + RPSI07_RS10540 0.22 +0.4
565,281 - RPSI07_RS10540 0.22 -2.5
565,462 + RPSI07_RS10540 0.30 -3.1
565,463 - RPSI07_RS10540 0.30 +0.1
565,614 + RPSI07_RS10540 0.38 -0.4
565,614 + RPSI07_RS10540 0.38 -2.1
565,614 + RPSI07_RS10540 0.38 +2.7
565,615 - RPSI07_RS10540 0.38 -2.0
565,615 - RPSI07_RS10540 0.38 -2.5
565,736 + RPSI07_RS10540 0.43 +2.0
565,737 - RPSI07_RS10540 0.43 +1.7
565,784 + RPSI07_RS10540 0.46 -1.1
565,976 + RPSI07_RS10540 0.55 -2.2
565,976 + RPSI07_RS10540 0.55 -0.1
565,976 + RPSI07_RS10540 0.55 +0.1
565,977 - RPSI07_RS10540 0.55 +1.0
565,977 - RPSI07_RS10540 0.55 +1.7
565,977 - RPSI07_RS10540 0.55 +0.7
565,977 - RPSI07_RS10540 0.55 -0.8
566,018 + RPSI07_RS10540 0.57 -0.6
566,019 - RPSI07_RS10540 0.57 +1.3
566,078 + RPSI07_RS10540 0.60 -0.7
566,078 + RPSI07_RS10540 0.60 -0.6
566,238 + RPSI07_RS10540 0.67 -0.4
566,238 + RPSI07_RS10540 0.67 +0.9
566,239 - RPSI07_RS10540 0.67 -0.3
566,319 - RPSI07_RS10540 0.71 -1.2
566,378 + RPSI07_RS10540 0.74 -2.5
566,426 + RPSI07_RS10540 0.76 -0.7
566,427 - RPSI07_RS10540 0.76 +2.7
566,427 - RPSI07_RS10540 0.76 -1.1
566,427 - RPSI07_RS10540 0.76 +1.1
566,477 + RPSI07_RS10540 0.79 -0.2
566,477 + RPSI07_RS10540 0.79 +0.2
566,478 - RPSI07_RS10540 0.79 -2.0
566,478 - RPSI07_RS10540 0.79 +0.2
566,492 + RPSI07_RS10540 0.79 -0.9
566,492 + RPSI07_RS10540 0.79 -1.2
566,492 + RPSI07_RS10540 0.79 +0.8
566,541 - RPSI07_RS10540 0.82 +0.3
566,541 - RPSI07_RS10540 0.82 +0.5
566,639 + RPSI07_RS10540 0.86 -2.0
566,640 - RPSI07_RS10540 0.86 +2.7
566,753 + -1.0
566,753 + -1.2
566,754 - +0.8
566,967 + +0.4
566,967 + -1.3
566,967 + -1.4
567,065 + +0.6
567,065 + +0.5
567,065 + -2.2
567,065 + +0.7
567,065 + +0.8
567,066 - +0.5
567,066 - +0.0
567,066 - +0.9
567,066 - -0.1
567,761 + RPSI07_RS10545 0.16 -0.1
567,818 - RPSI07_RS10545 0.19 +0.7
567,818 - RPSI07_RS10545 0.19 -1.0
567,845 - RPSI07_RS10545 0.21 -0.9

Or see this region's nucleotide sequence