Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS05490 and RPSI07_RS05495 are separated by 54 nucleotides RPSI07_RS05495 and RPSI07_RS05500 are separated by 119 nucleotides
RPSI07_RS05490: RPSI07_RS05490 - glycosyltransferase family 39 protein, at 1,493,267 to 1,494,922
_RS05490
RPSI07_RS05495: RPSI07_RS05495 - DUF333 domain-containing protein, at 1,494,977 to 1,495,411
_RS05495
RPSI07_RS05500: RPSI07_RS05500 - two-component sensor histidine kinase, at 1,495,531 to 1,496,817
_RS05500
Position (kb)
1494
1495
1496 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1494.041 kb on + strand, within RPSI07_RS05490 at 1494.041 kb on + strand, within RPSI07_RS05490 at 1494.041 kb on + strand, within RPSI07_RS05490 at 1494.042 kb on - strand, within RPSI07_RS05490 at 1494.042 kb on - strand, within RPSI07_RS05490 at 1494.042 kb on - strand, within RPSI07_RS05490 at 1494.042 kb on - strand, within RPSI07_RS05490 at 1494.042 kb on - strand, within RPSI07_RS05490 at 1494.045 kb on - strand, within RPSI07_RS05490 at 1494.070 kb on - strand, within RPSI07_RS05490 at 1494.233 kb on + strand, within RPSI07_RS05490 at 1494.233 kb on + strand, within RPSI07_RS05490 at 1494.233 kb on + strand, within RPSI07_RS05490 at 1494.233 kb on + strand, within RPSI07_RS05490 at 1494.233 kb on + strand, within RPSI07_RS05490 at 1494.234 kb on - strand, within RPSI07_RS05490 at 1494.235 kb on + strand, within RPSI07_RS05490 at 1494.235 kb on + strand, within RPSI07_RS05490 at 1494.236 kb on - strand, within RPSI07_RS05490 at 1494.236 kb on - strand, within RPSI07_RS05490 at 1494.236 kb on - strand, within RPSI07_RS05490 at 1494.342 kb on + strand, within RPSI07_RS05490 at 1494.653 kb on + strand, within RPSI07_RS05490 at 1494.653 kb on + strand, within RPSI07_RS05490 at 1494.653 kb on + strand, within RPSI07_RS05490 at 1494.686 kb on + strand, within RPSI07_RS05490 at 1494.686 kb on + strand, within RPSI07_RS05490 at 1494.686 kb on + strand, within RPSI07_RS05490 at 1494.686 kb on + strand, within RPSI07_RS05490 at 1494.686 kb on + strand, within RPSI07_RS05490 at 1494.687 kb on - strand, within RPSI07_RS05490 at 1494.687 kb on - strand, within RPSI07_RS05490 at 1494.689 kb on + strand, within RPSI07_RS05490 at 1494.689 kb on + strand, within RPSI07_RS05490 at 1494.689 kb on + strand, within RPSI07_RS05490 at 1494.690 kb on - strand, within RPSI07_RS05490 at 1494.818 kb on + strand at 1494.818 kb on + strand at 1494.819 kb on - strand at 1494.931 kb on + strand at 1494.931 kb on + strand at 1494.932 kb on - strand at 1494.932 kb on - strand at 1494.978 kb on + strand at 1494.978 kb on + strand at 1494.979 kb on - strand at 1494.979 kb on - strand at 1495.107 kb on + strand, within RPSI07_RS05495 at 1495.507 kb on - strand at 1495.511 kb on + strand at 1495.512 kb on - strand at 1495.512 kb on - strand at 1496.013 kb on - strand, within RPSI07_RS05500 at 1496.235 kb on - strand, within RPSI07_RS05500 at 1496.264 kb on + strand, within RPSI07_RS05500 at 1496.265 kb on - strand, within RPSI07_RS05500 at 1496.265 kb on - strand, within RPSI07_RS05500 at 1496.265 kb on - strand, within RPSI07_RS05500 at 1496.265 kb on - strand, within RPSI07_RS05500
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 1,494,041 + RPSI07_RS05490 0.47 -3.2 1,494,041 + RPSI07_RS05490 0.47 -3.4 1,494,041 + RPSI07_RS05490 0.47 -2.2 1,494,042 - RPSI07_RS05490 0.47 -4.6 1,494,042 - RPSI07_RS05490 0.47 -2.1 1,494,042 - RPSI07_RS05490 0.47 -3.5 1,494,042 - RPSI07_RS05490 0.47 +1.4 1,494,042 - RPSI07_RS05490 0.47 -3.2 1,494,045 - RPSI07_RS05490 0.47 -1.0 1,494,070 - RPSI07_RS05490 0.48 -2.9 1,494,233 + RPSI07_RS05490 0.58 -0.6 1,494,233 + RPSI07_RS05490 0.58 -4.4 1,494,233 + RPSI07_RS05490 0.58 -3.2 1,494,233 + RPSI07_RS05490 0.58 -3.5 1,494,233 + RPSI07_RS05490 0.58 -1.7 1,494,234 - RPSI07_RS05490 0.58 -3.9 1,494,235 + RPSI07_RS05490 0.58 -3.0 1,494,235 + RPSI07_RS05490 0.58 -2.5 1,494,236 - RPSI07_RS05490 0.59 -4.0 1,494,236 - RPSI07_RS05490 0.59 -2.2 1,494,236 - RPSI07_RS05490 0.59 +1.4 1,494,342 + RPSI07_RS05490 0.65 -2.1 1,494,653 + RPSI07_RS05490 0.84 -3.7 1,494,653 + RPSI07_RS05490 0.84 -2.5 1,494,653 + RPSI07_RS05490 0.84 -3.2 1,494,686 + RPSI07_RS05490 0.86 -1.9 1,494,686 + RPSI07_RS05490 0.86 -3.7 1,494,686 + RPSI07_RS05490 0.86 -2.9 1,494,686 + RPSI07_RS05490 0.86 -4.1 1,494,686 + RPSI07_RS05490 0.86 -1.6 1,494,687 - RPSI07_RS05490 0.86 -3.8 1,494,687 - RPSI07_RS05490 0.86 -2.8 1,494,689 + RPSI07_RS05490 0.86 -2.7 1,494,689 + RPSI07_RS05490 0.86 -2.0 1,494,689 + RPSI07_RS05490 0.86 -3.9 1,494,690 - RPSI07_RS05490 0.86 -4.2 1,494,818 + -4.5 1,494,818 + -3.2 1,494,819 - -2.4 1,494,931 + -0.0 1,494,931 + -0.4 1,494,932 - +0.3 1,494,932 - -0.2 1,494,978 + -0.5 1,494,978 + -2.7 1,494,979 - +0.4 1,494,979 - +0.6 1,495,107 + RPSI07_RS05495 0.30 -2.3 1,495,507 - -1.3 1,495,511 + -0.3 1,495,512 - +0.5 1,495,512 - -0.7 1,496,013 - RPSI07_RS05500 0.37 -2.9 1,496,235 - RPSI07_RS05500 0.55 -1.6 1,496,264 + RPSI07_RS05500 0.57 +1.4 1,496,265 - RPSI07_RS05500 0.57 +0.2 1,496,265 - RPSI07_RS05500 0.57 +1.0 1,496,265 - RPSI07_RS05500 0.57 +0.6 1,496,265 - RPSI07_RS05500 0.57 +0.1
Or see this region's nucleotide sequence