Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS04205 and RPSI07_RS04210 overlap by 1 nucleotides RPSI07_RS04210 and RPSI07_RS04215 are separated by 38 nucleotides RPSI07_RS04215 and RPSI07_RS04220 are separated by 119 nucleotides RPSI07_RS04220 and RPSI07_RS04225 are separated by 9 nucleotides
RPSI07_RS04205: RPSI07_RS04205 - xanthine dehydrogenase family protein molybdopterin-binding subunit, at 1,130,344 to 1,132,551
_RS04205
RPSI07_RS04210: RPSI07_RS04210 - carbon monoxide dehydrogenase accessory protein (coxi), at 1,132,551 to 1,133,558
_RS04210
RPSI07_RS04215: RPSI07_RS04215 - cytochrome c, at 1,133,597 to 1,133,914
_RS04215
RPSI07_RS04220: RPSI07_RS04220 - hypothetical protein, at 1,134,034 to 1,134,387
_RS04220
RPSI07_RS04225: RPSI07_RS04225 - hypothetical protein, at 1,134,397 to 1,134,696
_RS04225
Position (kb) 1132
1133
1134 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 1131.580 kb on + strand, within RPSI07_RS04205 at 1131.580 kb on + strand, within RPSI07_RS04205 at 1131.580 kb on + strand, within RPSI07_RS04205 at 1131.581 kb on - strand, within RPSI07_RS04205 at 1131.581 kb on - strand, within RPSI07_RS04205 at 1131.673 kb on + strand, within RPSI07_RS04205 at 1131.674 kb on - strand, within RPSI07_RS04205 at 1131.674 kb on - strand, within RPSI07_RS04205 at 1131.674 kb on - strand, within RPSI07_RS04205 at 1131.674 kb on - strand, within RPSI07_RS04205 at 1131.676 kb on + strand, within RPSI07_RS04205 at 1131.677 kb on - strand, within RPSI07_RS04205 at 1131.777 kb on + strand, within RPSI07_RS04205 at 1131.778 kb on - strand, within RPSI07_RS04205 at 1131.778 kb on - strand, within RPSI07_RS04205 at 1131.778 kb on - strand, within RPSI07_RS04205 at 1131.778 kb on - strand, within RPSI07_RS04205 at 1131.778 kb on - strand, within RPSI07_RS04205 at 1131.848 kb on - strand at 1131.943 kb on + strand, within RPSI07_RS04205 at 1131.943 kb on + strand, within RPSI07_RS04205 at 1131.944 kb on - strand, within RPSI07_RS04205 at 1131.944 kb on - strand, within RPSI07_RS04205 at 1132.337 kb on + strand at 1132.338 kb on - strand at 1132.338 kb on - strand at 1132.338 kb on - strand at 1132.338 kb on - strand at 1132.363 kb on + strand at 1132.363 kb on + strand at 1132.363 kb on + strand at 1132.392 kb on + strand at 1132.393 kb on - strand at 1132.542 kb on + strand at 1132.543 kb on - strand at 1132.748 kb on + strand, within RPSI07_RS04210 at 1132.795 kb on - strand, within RPSI07_RS04210 at 1133.077 kb on - strand, within RPSI07_RS04210 at 1133.077 kb on - strand, within RPSI07_RS04210 at 1133.107 kb on - strand, within RPSI07_RS04210 at 1133.118 kb on + strand, within RPSI07_RS04210 at 1133.139 kb on + strand, within RPSI07_RS04210 at 1133.140 kb on - strand, within RPSI07_RS04210 at 1133.140 kb on - strand, within RPSI07_RS04210 at 1133.304 kb on + strand, within RPSI07_RS04210 at 1133.304 kb on + strand, within RPSI07_RS04210 at 1133.304 kb on + strand, within RPSI07_RS04210 at 1133.304 kb on + strand, within RPSI07_RS04210 at 1133.304 kb on + strand, within RPSI07_RS04210 at 1133.305 kb on - strand, within RPSI07_RS04210 at 1133.305 kb on - strand, within RPSI07_RS04210 at 1133.305 kb on - strand, within RPSI07_RS04210 at 1133.365 kb on - strand, within RPSI07_RS04210 at 1133.521 kb on + strand at 1133.521 kb on + strand at 1133.600 kb on + strand at 1133.600 kb on + strand at 1133.600 kb on + strand at 1133.600 kb on + strand at 1133.600 kb on + strand at 1133.600 kb on + strand at 1133.601 kb on - strand at 1133.601 kb on - strand at 1133.601 kb on - strand at 1133.681 kb on + strand, within RPSI07_RS04215 at 1133.681 kb on + strand, within RPSI07_RS04215 at 1133.682 kb on - strand, within RPSI07_RS04215 at 1133.682 kb on - strand, within RPSI07_RS04215 at 1133.753 kb on + strand, within RPSI07_RS04215 at 1133.753 kb on + strand, within RPSI07_RS04215 at 1133.754 kb on - strand, within RPSI07_RS04215 at 1133.754 kb on - strand, within RPSI07_RS04215 at 1133.754 kb on - strand, within RPSI07_RS04215 at 1133.754 kb on - strand, within RPSI07_RS04215 at 1133.754 kb on - strand, within RPSI07_RS04215 at 1133.816 kb on - strand, within RPSI07_RS04215 at 1133.945 kb on + strand at 1133.946 kb on - strand at 1133.946 kb on - strand at 1134.145 kb on + strand, within RPSI07_RS04220 at 1134.145 kb on + strand, within RPSI07_RS04220 at 1134.146 kb on - strand, within RPSI07_RS04220 at 1134.146 kb on - strand, within RPSI07_RS04220 at 1134.146 kb on - strand, within RPSI07_RS04220 at 1134.169 kb on + strand, within RPSI07_RS04220 at 1134.233 kb on + strand, within RPSI07_RS04220 at 1134.233 kb on + strand, within RPSI07_RS04220 at 1134.233 kb on + strand, within RPSI07_RS04220 at 1134.233 kb on + strand, within RPSI07_RS04220 at 1134.234 kb on - strand, within RPSI07_RS04220 at 1134.234 kb on - strand, within RPSI07_RS04220 at 1134.234 kb on - strand, within RPSI07_RS04220 at 1134.238 kb on + strand, within RPSI07_RS04220 at 1134.238 kb on + strand, within RPSI07_RS04220 at 1134.238 kb on + strand, within RPSI07_RS04220 at 1134.238 kb on + strand, within RPSI07_RS04220 at 1134.238 kb on + strand, within RPSI07_RS04220 at 1134.238 kb on + strand, within RPSI07_RS04220 at 1134.238 kb on + strand, within RPSI07_RS04220 at 1134.238 kb on + strand, within RPSI07_RS04220 at 1134.239 kb on - strand, within RPSI07_RS04220 at 1134.239 kb on - strand, within RPSI07_RS04220 at 1134.239 kb on - strand, within RPSI07_RS04220 at 1134.239 kb on - strand, within RPSI07_RS04220 at 1134.239 kb on - strand, within RPSI07_RS04220 at 1134.239 kb on - strand, within RPSI07_RS04220 at 1134.239 kb on - strand, within RPSI07_RS04220 at 1134.239 kb on - strand, within RPSI07_RS04220 at 1134.239 kb on - strand, within RPSI07_RS04220 at 1134.241 kb on + strand, within RPSI07_RS04220 at 1134.242 kb on - strand, within RPSI07_RS04220 at 1134.271 kb on + strand, within RPSI07_RS04220 at 1134.271 kb on + strand, within RPSI07_RS04220 at 1134.271 kb on + strand, within RPSI07_RS04220 at 1134.271 kb on + strand, within RPSI07_RS04220 at 1134.271 kb on + strand, within RPSI07_RS04220 at 1134.271 kb on + strand, within RPSI07_RS04220 at 1134.272 kb on - strand, within RPSI07_RS04220 at 1134.272 kb on - strand, within RPSI07_RS04220 at 1134.376 kb on - strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.385 kb on + strand at 1134.386 kb on - strand at 1134.386 kb on - strand at 1134.386 kb on - strand at 1134.386 kb on - strand at 1134.386 kb on - strand at 1134.386 kb on - strand at 1134.386 kb on - strand at 1134.386 kb on - strand at 1134.386 kb on - strand at 1134.386 kb on - strand at 1134.502 kb on + strand, within RPSI07_RS04225 at 1134.503 kb on - strand, within RPSI07_RS04225 at 1134.503 kb on - strand, within RPSI07_RS04225 at 1134.503 kb on - strand, within RPSI07_RS04225 at 1134.503 kb on - strand, within RPSI07_RS04225
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 1,131,580 + RPSI07_RS04205 0.56 +0.4 1,131,580 + RPSI07_RS04205 0.56 -0.6 1,131,580 + RPSI07_RS04205 0.56 -0.9 1,131,581 - RPSI07_RS04205 0.56 -0.7 1,131,581 - RPSI07_RS04205 0.56 +0.7 1,131,673 + RPSI07_RS04205 0.60 -1.5 1,131,674 - RPSI07_RS04205 0.60 +0.7 1,131,674 - RPSI07_RS04205 0.60 +1.9 1,131,674 - RPSI07_RS04205 0.60 -0.6 1,131,674 - RPSI07_RS04205 0.60 +0.1 1,131,676 + RPSI07_RS04205 0.60 +1.8 1,131,677 - RPSI07_RS04205 0.60 +0.1 1,131,777 + RPSI07_RS04205 0.65 -0.3 1,131,778 - RPSI07_RS04205 0.65 -0.6 1,131,778 - RPSI07_RS04205 0.65 -2.5 1,131,778 - RPSI07_RS04205 0.65 +0.6 1,131,778 - RPSI07_RS04205 0.65 +0.1 1,131,778 - RPSI07_RS04205 0.65 +0.9 1,131,848 - +1.9 1,131,943 + RPSI07_RS04205 0.72 -1.0 1,131,943 + RPSI07_RS04205 0.72 +0.6 1,131,944 - RPSI07_RS04205 0.72 -1.0 1,131,944 - RPSI07_RS04205 0.72 +3.1 1,132,337 + +0.1 1,132,338 - -0.5 1,132,338 - -1.8 1,132,338 - -0.8 1,132,338 - -0.9 1,132,363 + -0.8 1,132,363 + +0.2 1,132,363 + +2.3 1,132,392 + +0.0 1,132,393 - -1.0 1,132,542 + -1.0 1,132,543 - -0.6 1,132,748 + RPSI07_RS04210 0.20 +0.2 1,132,795 - RPSI07_RS04210 0.24 +0.6 1,133,077 - RPSI07_RS04210 0.52 +1.3 1,133,077 - RPSI07_RS04210 0.52 +1.8 1,133,107 - RPSI07_RS04210 0.55 +1.3 1,133,118 + RPSI07_RS04210 0.56 +0.2 1,133,139 + RPSI07_RS04210 0.58 -0.3 1,133,140 - RPSI07_RS04210 0.58 -0.2 1,133,140 - RPSI07_RS04210 0.58 +1.3 1,133,304 + RPSI07_RS04210 0.75 -0.1 1,133,304 + RPSI07_RS04210 0.75 -0.4 1,133,304 + RPSI07_RS04210 0.75 +0.3 1,133,304 + RPSI07_RS04210 0.75 -1.2 1,133,304 + RPSI07_RS04210 0.75 -0.7 1,133,305 - RPSI07_RS04210 0.75 +0.8 1,133,305 - RPSI07_RS04210 0.75 -0.1 1,133,305 - RPSI07_RS04210 0.75 -0.5 1,133,365 - RPSI07_RS04210 0.81 -0.4 1,133,521 + +0.8 1,133,521 + +6.1 1,133,600 + +0.2 1,133,600 + -2.0 1,133,600 + -2.3 1,133,600 + -0.6 1,133,600 + +0.0 1,133,600 + -2.0 1,133,601 - -0.2 1,133,601 - -2.1 1,133,601 - +0.1 1,133,681 + RPSI07_RS04215 0.26 -0.6 1,133,681 + RPSI07_RS04215 0.26 -1.7 1,133,682 - RPSI07_RS04215 0.27 -0.9 1,133,682 - RPSI07_RS04215 0.27 -0.7 1,133,753 + RPSI07_RS04215 0.49 +0.6 1,133,753 + RPSI07_RS04215 0.49 -0.7 1,133,754 - RPSI07_RS04215 0.49 -0.4 1,133,754 - RPSI07_RS04215 0.49 -1.3 1,133,754 - RPSI07_RS04215 0.49 +0.1 1,133,754 - RPSI07_RS04215 0.49 -0.0 1,133,754 - RPSI07_RS04215 0.49 +1.2 1,133,816 - RPSI07_RS04215 0.69 -0.4 1,133,945 + -0.7 1,133,946 - -1.3 1,133,946 - -0.6 1,134,145 + RPSI07_RS04220 0.31 -0.6 1,134,145 + RPSI07_RS04220 0.31 +0.1 1,134,146 - RPSI07_RS04220 0.32 -0.4 1,134,146 - RPSI07_RS04220 0.32 +0.3 1,134,146 - RPSI07_RS04220 0.32 +0.4 1,134,169 + RPSI07_RS04220 0.38 +1.4 1,134,233 + RPSI07_RS04220 0.56 -0.1 1,134,233 + RPSI07_RS04220 0.56 +0.4 1,134,233 + RPSI07_RS04220 0.56 +0.2 1,134,233 + RPSI07_RS04220 0.56 -0.9 1,134,234 - RPSI07_RS04220 0.56 +0.5 1,134,234 - RPSI07_RS04220 0.56 +0.2 1,134,234 - RPSI07_RS04220 0.56 -0.3 1,134,238 + RPSI07_RS04220 0.58 +0.5 1,134,238 + RPSI07_RS04220 0.58 +1.1 1,134,238 + RPSI07_RS04220 0.58 -1.0 1,134,238 + RPSI07_RS04220 0.58 +0.6 1,134,238 + RPSI07_RS04220 0.58 -0.8 1,134,238 + RPSI07_RS04220 0.58 +0.9 1,134,238 + RPSI07_RS04220 0.58 +1.3 1,134,238 + RPSI07_RS04220 0.58 -1.0 1,134,239 - RPSI07_RS04220 0.58 +0.2 1,134,239 - RPSI07_RS04220 0.58 -0.7 1,134,239 - RPSI07_RS04220 0.58 +0.8 1,134,239 - RPSI07_RS04220 0.58 -2.3 1,134,239 - RPSI07_RS04220 0.58 +0.3 1,134,239 - RPSI07_RS04220 0.58 +0.7 1,134,239 - RPSI07_RS04220 0.58 +0.6 1,134,239 - RPSI07_RS04220 0.58 -1.2 1,134,239 - RPSI07_RS04220 0.58 -0.2 1,134,241 + RPSI07_RS04220 0.58 -0.4 1,134,242 - RPSI07_RS04220 0.59 -0.0 1,134,271 + RPSI07_RS04220 0.67 -1.5 1,134,271 + RPSI07_RS04220 0.67 +0.4 1,134,271 + RPSI07_RS04220 0.67 -0.3 1,134,271 + RPSI07_RS04220 0.67 +0.0 1,134,271 + RPSI07_RS04220 0.67 -1.3 1,134,271 + RPSI07_RS04220 0.67 +0.9 1,134,272 - RPSI07_RS04220 0.67 +3.1 1,134,272 - RPSI07_RS04220 0.67 +0.7 1,134,376 - +0.4 1,134,385 + -1.9 1,134,385 + +0.1 1,134,385 + -0.7 1,134,385 + +0.5 1,134,385 + +0.6 1,134,385 + -1.0 1,134,385 + +0.0 1,134,385 + +0.0 1,134,385 + -0.7 1,134,385 + +0.4 1,134,385 + -1.7 1,134,385 + +0.5 1,134,385 + -1.9 1,134,386 - -2.2 1,134,386 - -1.9 1,134,386 - -0.4 1,134,386 - +1.1 1,134,386 - +1.3 1,134,386 - -0.7 1,134,386 - -0.0 1,134,386 - -1.7 1,134,386 - -0.3 1,134,386 - +0.7 1,134,502 + RPSI07_RS04225 0.35 -0.9 1,134,503 - RPSI07_RS04225 0.35 -1.2 1,134,503 - RPSI07_RS04225 0.35 +1.6 1,134,503 - RPSI07_RS04225 0.35 -0.4 1,134,503 - RPSI07_RS04225 0.35 -1.2
Or see this region's nucleotide sequence