Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS24470 and RPSI07_RS24475 are separated by 93 nucleotides RPSI07_RS24475 and RPSI07_RS24480 are separated by 154 nucleotides RPSI07_RS24480 and RPSI07_RS05235 are separated by 722 nucleotides
RPSI07_RS24470: RPSI07_RS24470 - hypothetical protein, at 1,429,281 to 1,429,664
_RS24470
RPSI07_RS24475: RPSI07_RS24475 - hypothetical protein, at 1,429,758 to 1,430,231
_RS24475
RPSI07_RS24480: RPSI07_RS24480 - hypothetical protein, at 1,430,386 to 1,430,760
_RS24480
RPSI07_RS05235: RPSI07_RS05235 - hypothetical protein, at 1,431,483 to 1,435,601
_RS05235
Position (kb)
1430
1431 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1429.445 kb on - strand, within RPSI07_RS24470 at 1429.487 kb on + strand, within RPSI07_RS24470 at 1429.588 kb on + strand, within RPSI07_RS24470 at 1429.632 kb on - strand at 1429.655 kb on + strand at 1429.733 kb on + strand at 1429.755 kb on + strand at 1429.755 kb on + strand at 1429.801 kb on + strand at 1429.802 kb on - strand at 1429.833 kb on - strand, within RPSI07_RS24475 at 1429.841 kb on + strand, within RPSI07_RS24475 at 1429.842 kb on - strand, within RPSI07_RS24475 at 1429.847 kb on - strand, within RPSI07_RS24475 at 1429.923 kb on + strand, within RPSI07_RS24475 at 1429.941 kb on + strand, within RPSI07_RS24475 at 1429.949 kb on + strand, within RPSI07_RS24475 at 1429.961 kb on + strand, within RPSI07_RS24475 at 1429.989 kb on - strand, within RPSI07_RS24475 at 1430.030 kb on + strand, within RPSI07_RS24475 at 1430.048 kb on + strand, within RPSI07_RS24475 at 1430.077 kb on + strand, within RPSI07_RS24475 at 1430.078 kb on - strand, within RPSI07_RS24475 at 1430.122 kb on - strand, within RPSI07_RS24475 at 1430.141 kb on - strand, within RPSI07_RS24475 at 1430.141 kb on - strand, within RPSI07_RS24475 at 1430.166 kb on - strand, within RPSI07_RS24475 at 1430.205 kb on + strand at 1430.301 kb on + strand at 1430.381 kb on - strand at 1431.039 kb on + strand at 1431.039 kb on + strand at 1431.070 kb on + strand at 1431.156 kb on + strand at 1431.156 kb on + strand at 1431.157 kb on - strand at 1431.157 kb on - strand at 1431.157 kb on - strand at 1431.198 kb on + strand at 1431.198 kb on + strand at 1431.199 kb on - strand at 1431.199 kb on - strand at 1431.199 kb on - strand at 1431.299 kb on + strand at 1431.299 kb on + strand at 1431.300 kb on - strand at 1431.320 kb on + strand at 1431.362 kb on + strand at 1431.362 kb on + strand at 1431.362 kb on + strand at 1431.362 kb on + strand at 1431.363 kb on - strand at 1431.363 kb on - strand at 1431.363 kb on - strand at 1431.410 kb on + strand at 1431.411 kb on - strand at 1431.411 kb on - strand at 1431.428 kb on + strand at 1431.428 kb on + strand at 1431.428 kb on + strand at 1431.429 kb on - strand at 1431.429 kb on - strand at 1431.449 kb on + strand at 1431.450 kb on - strand at 1431.450 kb on - strand at 1431.536 kb on + strand at 1431.536 kb on + strand at 1431.536 kb on + strand at 1431.537 kb on - strand at 1431.537 kb on - strand at 1431.537 kb on - strand at 1431.538 kb on + strand at 1431.538 kb on + strand at 1431.538 kb on + strand at 1431.538 kb on + strand at 1431.538 kb on + strand at 1431.538 kb on + strand at 1431.539 kb on - strand at 1431.539 kb on - strand at 1431.539 kb on - strand at 1431.539 kb on - strand at 1431.547 kb on + strand at 1431.547 kb on + strand at 1431.547 kb on + strand at 1431.547 kb on + strand at 1431.547 kb on + strand at 1431.548 kb on - strand at 1431.548 kb on - strand at 1431.607 kb on + strand at 1431.607 kb on + strand at 1431.607 kb on + strand at 1431.607 kb on + strand at 1431.607 kb on + strand at 1431.608 kb on - strand at 1431.608 kb on - strand at 1431.608 kb on - strand at 1431.608 kb on - strand at 1431.658 kb on + strand at 1431.658 kb on + strand at 1431.658 kb on + strand at 1431.659 kb on - strand at 1431.659 kb on - strand at 1431.659 kb on - strand at 1431.659 kb on - strand at 1431.679 kb on + strand at 1431.679 kb on + strand at 1431.680 kb on - strand at 1431.680 kb on - strand at 1431.680 kb on - strand at 1431.680 kb on - strand at 1431.682 kb on + strand at 1431.697 kb on + strand at 1431.697 kb on + strand at 1431.697 kb on + strand at 1431.697 kb on + strand at 1431.698 kb on - strand at 1431.698 kb on - strand at 1431.698 kb on - strand at 1431.749 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,429,445 - RPSI07_RS24470 0.43 -0.9 1,429,487 + RPSI07_RS24470 0.54 -0.6 1,429,588 + RPSI07_RS24470 0.80 -1.5 1,429,632 - +0.5 1,429,655 + -1.0 1,429,733 + +1.1 1,429,755 + +0.9 1,429,755 + +1.1 1,429,801 + +0.5 1,429,802 - -1.2 1,429,833 - RPSI07_RS24475 0.16 +0.7 1,429,841 + RPSI07_RS24475 0.18 +0.6 1,429,842 - RPSI07_RS24475 0.18 -0.7 1,429,847 - RPSI07_RS24475 0.19 -1.5 1,429,923 + RPSI07_RS24475 0.35 -0.8 1,429,941 + RPSI07_RS24475 0.39 +0.8 1,429,949 + RPSI07_RS24475 0.40 +0.1 1,429,961 + RPSI07_RS24475 0.43 +0.5 1,429,989 - RPSI07_RS24475 0.49 -1.2 1,430,030 + RPSI07_RS24475 0.57 -1.1 1,430,048 + RPSI07_RS24475 0.61 -0.1 1,430,077 + RPSI07_RS24475 0.67 +0.5 1,430,078 - RPSI07_RS24475 0.68 -1.0 1,430,122 - RPSI07_RS24475 0.77 -1.1 1,430,141 - RPSI07_RS24475 0.81 +0.6 1,430,141 - RPSI07_RS24475 0.81 -0.5 1,430,166 - RPSI07_RS24475 0.86 +0.1 1,430,205 + -1.7 1,430,301 + +1.4 1,430,381 - +1.4 1,431,039 + +1.8 1,431,039 + -0.2 1,431,070 + -0.6 1,431,156 + -2.3 1,431,156 + -0.5 1,431,157 - -1.4 1,431,157 - -0.7 1,431,157 - +0.1 1,431,198 + +0.2 1,431,198 + +0.4 1,431,199 - -0.6 1,431,199 - -0.6 1,431,199 - -1.0 1,431,299 + +2.7 1,431,299 + +1.3 1,431,300 - -0.4 1,431,320 + -0.3 1,431,362 + -2.1 1,431,362 + -0.9 1,431,362 + +1.2 1,431,362 + -0.3 1,431,363 - +0.0 1,431,363 - -1.7 1,431,363 - +0.2 1,431,410 + +0.1 1,431,411 - -0.2 1,431,411 - -0.1 1,431,428 + -0.9 1,431,428 + -0.3 1,431,428 + +0.1 1,431,429 - -1.7 1,431,429 - +0.6 1,431,449 + +0.5 1,431,450 - -0.4 1,431,450 - +0.3 1,431,536 + -0.5 1,431,536 + -0.7 1,431,536 + +0.7 1,431,537 - -0.1 1,431,537 - +0.0 1,431,537 - +0.7 1,431,538 + -0.8 1,431,538 + -0.3 1,431,538 + -1.2 1,431,538 + +2.7 1,431,538 + +0.5 1,431,538 + -2.3 1,431,539 - -0.3 1,431,539 - +0.7 1,431,539 - +0.7 1,431,539 - +0.1 1,431,547 + +0.6 1,431,547 + -1.0 1,431,547 + -0.4 1,431,547 + -1.3 1,431,547 + +0.6 1,431,548 - -0.7 1,431,548 - +0.1 1,431,607 + +1.1 1,431,607 + -0.6 1,431,607 + -0.1 1,431,607 + -0.0 1,431,607 + +0.3 1,431,608 - +1.6 1,431,608 - +0.4 1,431,608 - -1.4 1,431,608 - -1.1 1,431,658 + -3.8 1,431,658 + -2.0 1,431,658 + +0.1 1,431,659 - +0.7 1,431,659 - +0.1 1,431,659 - -0.6 1,431,659 - -1.6 1,431,679 + +2.1 1,431,679 + +0.1 1,431,680 - -1.8 1,431,680 - -0.4 1,431,680 - -0.2 1,431,680 - -0.8 1,431,682 + -2.1 1,431,697 + +0.8 1,431,697 + +2.7 1,431,697 + -0.9 1,431,697 + +0.1 1,431,698 - -0.9 1,431,698 - +0.2 1,431,698 - -0.1 1,431,749 + +0.8
Or see this region's nucleotide sequence