Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS23915 and RPSI07_RS23920 are separated by 705 nucleotides RPSI07_RS23920 and RPSI07_RS23930 are separated by 115 nucleotides
RPSI07_RS23915: RPSI07_RS23915 - 30S ribosomal protein S21, at 3,511,241 to 3,511,453
_RS23915
RPSI07_RS23920: RPSI07_RS23920 - IS3 family transposase, at 3,512,159 to 3,513,276
_RS23920
RPSI07_RS23930: RPSI07_RS23930 - DUF3800 domain-containing protein, at 3,513,392 to 3,514,069
_RS23930
Position (kb)
3512
3513
3514 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3511.621 kb on - strand at 3511.850 kb on + strand at 3511.850 kb on + strand at 3511.851 kb on - strand at 3511.858 kb on + strand at 3511.858 kb on + strand at 3511.885 kb on - strand at 3511.964 kb on - strand at 3511.992 kb on + strand at 3512.146 kb on - strand at 3512.146 kb on - strand at 3512.146 kb on - strand at 3512.146 kb on - strand at 3513.305 kb on + strand at 3513.308 kb on + strand at 3513.329 kb on + strand at 3513.357 kb on + strand at 3513.357 kb on + strand at 3513.358 kb on - strand at 3513.358 kb on - strand at 3513.358 kb on - strand at 3513.358 kb on - strand at 3513.358 kb on - strand at 3513.363 kb on + strand at 3513.363 kb on + strand at 3513.363 kb on + strand at 3513.364 kb on - strand at 3513.452 kb on + strand at 3513.452 kb on + strand at 3513.452 kb on + strand at 3513.452 kb on + strand at 3513.453 kb on - strand at 3513.453 kb on - strand at 3513.453 kb on - strand at 3513.481 kb on - strand, within RPSI07_RS23930 at 3513.482 kb on - strand, within RPSI07_RS23930 at 3513.523 kb on + strand, within RPSI07_RS23930 at 3513.523 kb on + strand, within RPSI07_RS23930 at 3513.596 kb on + strand, within RPSI07_RS23930 at 3513.596 kb on + strand, within RPSI07_RS23930 at 3513.597 kb on - strand, within RPSI07_RS23930 at 3513.597 kb on - strand, within RPSI07_RS23930 at 3513.597 kb on - strand, within RPSI07_RS23930 at 3513.620 kb on + strand, within RPSI07_RS23930 at 3513.642 kb on + strand, within RPSI07_RS23930 at 3513.643 kb on - strand, within RPSI07_RS23930 at 3513.643 kb on - strand, within RPSI07_RS23930 at 3513.643 kb on - strand, within RPSI07_RS23930 at 3513.643 kb on - strand, within RPSI07_RS23930 at 3513.666 kb on + strand, within RPSI07_RS23930 at 3513.666 kb on + strand, within RPSI07_RS23930 at 3513.666 kb on + strand, within RPSI07_RS23930 at 3513.667 kb on - strand, within RPSI07_RS23930 at 3513.667 kb on - strand, within RPSI07_RS23930 at 3513.669 kb on + strand, within RPSI07_RS23930 at 3513.669 kb on + strand, within RPSI07_RS23930 at 3513.670 kb on - strand, within RPSI07_RS23930 at 3513.670 kb on - strand, within RPSI07_RS23930 at 3513.670 kb on - strand, within RPSI07_RS23930 at 3513.670 kb on - strand, within RPSI07_RS23930 at 3513.719 kb on + strand, within RPSI07_RS23930 at 3513.719 kb on + strand, within RPSI07_RS23930 at 3513.719 kb on + strand, within RPSI07_RS23930 at 3513.719 kb on + strand, within RPSI07_RS23930 at 3513.720 kb on - strand, within RPSI07_RS23930 at 3513.720 kb on - strand, within RPSI07_RS23930 at 3513.720 kb on - strand, within RPSI07_RS23930 at 3513.720 kb on - strand, within RPSI07_RS23930 at 3513.720 kb on - strand, within RPSI07_RS23930 at 3513.767 kb on + strand, within RPSI07_RS23930 at 3513.767 kb on + strand, within RPSI07_RS23930 at 3513.767 kb on + strand, within RPSI07_RS23930 at 3513.767 kb on + strand, within RPSI07_RS23930 at 3513.767 kb on + strand, within RPSI07_RS23930 at 3513.768 kb on - strand, within RPSI07_RS23930 at 3513.778 kb on + strand, within RPSI07_RS23930 at 3513.778 kb on + strand, within RPSI07_RS23930 at 3513.779 kb on - strand at 3513.779 kb on - strand, within RPSI07_RS23930 at 3513.779 kb on - strand, within RPSI07_RS23930 at 3513.821 kb on + strand, within RPSI07_RS23930 at 3513.822 kb on - strand, within RPSI07_RS23930 at 3513.822 kb on - strand, within RPSI07_RS23930 at 3513.860 kb on + strand, within RPSI07_RS23930 at 3513.880 kb on + strand, within RPSI07_RS23930 at 3513.881 kb on - strand, within RPSI07_RS23930 at 3513.903 kb on + strand, within RPSI07_RS23930 at 3513.904 kb on - strand, within RPSI07_RS23930 at 3513.941 kb on + strand, within RPSI07_RS23930 at 3513.942 kb on - strand, within RPSI07_RS23930 at 3513.963 kb on - strand, within RPSI07_RS23930 at 3514.002 kb on + strand at 3514.002 kb on + strand at 3514.002 kb on + strand at 3514.002 kb on + strand at 3514.003 kb on - strand at 3514.003 kb on - strand at 3514.003 kb on - strand at 3514.003 kb on - strand at 3514.003 kb on - strand at 3514.003 kb on - strand at 3514.003 kb on - strand at 3514.023 kb on + strand at 3514.024 kb on - strand at 3514.033 kb on + strand at 3514.033 kb on + strand at 3514.033 kb on + strand at 3514.033 kb on + strand at 3514.033 kb on + strand at 3514.034 kb on - strand at 3514.034 kb on - strand at 3514.034 kb on - strand at 3514.034 kb on - strand at 3514.034 kb on - strand at 3514.037 kb on + strand at 3514.037 kb on + strand at 3514.037 kb on + strand at 3514.037 kb on + strand at 3514.037 kb on + strand at 3514.038 kb on - strand at 3514.038 kb on - strand at 3514.038 kb on - strand at 3514.038 kb on - strand at 3514.067 kb on + strand at 3514.091 kb on - strand at 3514.092 kb on + strand at 3514.093 kb on - strand at 3514.093 kb on - strand at 3514.093 kb on - strand at 3514.118 kb on - strand at 3514.118 kb on - strand at 3514.118 kb on - strand at 3514.118 kb on - strand at 3514.118 kb on - strand at 3514.135 kb on + strand at 3514.135 kb on + strand at 3514.135 kb on + strand at 3514.135 kb on + strand at 3514.136 kb on - strand at 3514.140 kb on + strand at 3514.183 kb on + strand at 3514.183 kb on + strand at 3514.183 kb on + strand at 3514.183 kb on + strand at 3514.184 kb on - strand at 3514.184 kb on - strand at 3514.185 kb on + strand at 3514.185 kb on + strand at 3514.185 kb on + strand at 3514.186 kb on - strand at 3514.186 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,511,621 - +1.4 3,511,850 + +1.7 3,511,850 + +0.2 3,511,851 - -1.5 3,511,858 + -0.4 3,511,858 + +0.8 3,511,885 - +1.0 3,511,964 - -0.1 3,511,992 + -1.6 3,512,146 - +1.1 3,512,146 - +0.2 3,512,146 - -1.6 3,512,146 - -0.5 3,513,305 + -0.7 3,513,308 + +0.2 3,513,329 + +2.0 3,513,357 + -0.9 3,513,357 + +0.1 3,513,358 - +1.4 3,513,358 - +0.1 3,513,358 - +0.6 3,513,358 - -1.5 3,513,358 - -0.3 3,513,363 + +2.0 3,513,363 + +0.3 3,513,363 + +1.1 3,513,364 - +0.5 3,513,452 + +0.0 3,513,452 + -2.1 3,513,452 + -0.4 3,513,452 + +3.2 3,513,453 - +1.4 3,513,453 - +0.5 3,513,453 - -0.1 3,513,481 - RPSI07_RS23930 0.13 -0.6 3,513,482 - RPSI07_RS23930 0.13 -0.3 3,513,523 + RPSI07_RS23930 0.19 -0.5 3,513,523 + RPSI07_RS23930 0.19 -0.7 3,513,596 + RPSI07_RS23930 0.30 -0.9 3,513,596 + RPSI07_RS23930 0.30 +0.2 3,513,597 - RPSI07_RS23930 0.30 +0.0 3,513,597 - RPSI07_RS23930 0.30 +0.5 3,513,597 - RPSI07_RS23930 0.30 +0.4 3,513,620 + RPSI07_RS23930 0.34 -0.2 3,513,642 + RPSI07_RS23930 0.37 +2.9 3,513,643 - RPSI07_RS23930 0.37 -0.2 3,513,643 - RPSI07_RS23930 0.37 +1.4 3,513,643 - RPSI07_RS23930 0.37 +1.7 3,513,643 - RPSI07_RS23930 0.37 +0.4 3,513,666 + RPSI07_RS23930 0.40 +2.1 3,513,666 + RPSI07_RS23930 0.40 +0.1 3,513,666 + RPSI07_RS23930 0.40 -0.3 3,513,667 - RPSI07_RS23930 0.41 -0.1 3,513,667 - RPSI07_RS23930 0.41 -0.3 3,513,669 + RPSI07_RS23930 0.41 -0.9 3,513,669 + RPSI07_RS23930 0.41 +0.2 3,513,670 - RPSI07_RS23930 0.41 -0.2 3,513,670 - RPSI07_RS23930 0.41 +2.4 3,513,670 - RPSI07_RS23930 0.41 +0.2 3,513,670 - RPSI07_RS23930 0.41 +0.1 3,513,719 + RPSI07_RS23930 0.48 -0.3 3,513,719 + RPSI07_RS23930 0.48 +0.5 3,513,719 + RPSI07_RS23930 0.48 -0.8 3,513,719 + RPSI07_RS23930 0.48 -0.3 3,513,720 - RPSI07_RS23930 0.48 -1.3 3,513,720 - RPSI07_RS23930 0.48 -0.9 3,513,720 - RPSI07_RS23930 0.48 -0.6 3,513,720 - RPSI07_RS23930 0.48 -2.4 3,513,720 - RPSI07_RS23930 0.48 +0.3 3,513,767 + RPSI07_RS23930 0.55 -0.5 3,513,767 + RPSI07_RS23930 0.55 +2.4 3,513,767 + RPSI07_RS23930 0.55 -2.7 3,513,767 + RPSI07_RS23930 0.55 +0.2 3,513,767 + RPSI07_RS23930 0.55 +0.5 3,513,768 - RPSI07_RS23930 0.55 +1.8 3,513,778 + RPSI07_RS23930 0.57 -0.1 3,513,778 + RPSI07_RS23930 0.57 -0.6 3,513,779 - +2.7 3,513,779 - RPSI07_RS23930 0.57 -0.9 3,513,779 - RPSI07_RS23930 0.57 -0.9 3,513,821 + RPSI07_RS23930 0.63 +2.4 3,513,822 - RPSI07_RS23930 0.63 -2.3 3,513,822 - RPSI07_RS23930 0.63 +5.6 3,513,860 + RPSI07_RS23930 0.69 +0.1 3,513,880 + RPSI07_RS23930 0.72 -1.5 3,513,881 - RPSI07_RS23930 0.72 +0.6 3,513,903 + RPSI07_RS23930 0.75 -0.3 3,513,904 - RPSI07_RS23930 0.76 +0.8 3,513,941 + RPSI07_RS23930 0.81 +1.6 3,513,942 - RPSI07_RS23930 0.81 -1.4 3,513,963 - RPSI07_RS23930 0.84 +1.1 3,514,002 + -0.8 3,514,002 + -2.3 3,514,002 + -2.4 3,514,002 + -1.2 3,514,003 - +0.8 3,514,003 - -0.3 3,514,003 - +0.6 3,514,003 - -0.7 3,514,003 - -0.1 3,514,003 - -0.1 3,514,003 - +1.5 3,514,023 + -1.1 3,514,024 - -1.1 3,514,033 + +0.5 3,514,033 + +1.4 3,514,033 + +0.4 3,514,033 + -1.4 3,514,033 + -1.3 3,514,034 - +0.1 3,514,034 - +0.8 3,514,034 - +0.0 3,514,034 - +1.1 3,514,034 - +0.4 3,514,037 + +0.8 3,514,037 + +0.4 3,514,037 + +0.5 3,514,037 + +0.2 3,514,037 + -0.6 3,514,038 - -0.9 3,514,038 - +0.6 3,514,038 - -0.2 3,514,038 - -0.3 3,514,067 + -1.6 3,514,091 - +2.0 3,514,092 + -0.5 3,514,093 - -1.0 3,514,093 - -0.6 3,514,093 - -1.9 3,514,118 - +0.4 3,514,118 - +0.4 3,514,118 - -0.5 3,514,118 - +0.3 3,514,118 - -1.6 3,514,135 + -0.5 3,514,135 + +0.8 3,514,135 + +0.1 3,514,135 + -1.9 3,514,136 - -0.6 3,514,140 + +2.3 3,514,183 + +0.5 3,514,183 + +1.4 3,514,183 + -0.9 3,514,183 + -0.5 3,514,184 - +3.7 3,514,184 - +1.4 3,514,185 + -3.6 3,514,185 + -0.7 3,514,185 + -0.3 3,514,186 - +0.8 3,514,186 - +0.3
Or see this region's nucleotide sequence