Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS20435

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS20430 and RPSI07_RS20435 are separated by 124 nucleotidesRPSI07_RS20435 and RPSI07_RS20440 are separated by 68 nucleotides RPSI07_RS20430: RPSI07_RS20430 - 4-hydroxy-3-polyprenylbenzoate decarboxylase, at 2,760,405 to 2,761,916 _RS20430 RPSI07_RS20435: RPSI07_RS20435 - NAD-dependent succinate-semialdehyde dehydrogenase, at 2,762,041 to 2,763,558 _RS20435 RPSI07_RS20440: RPSI07_RS20440 - acetoacetate--CoA ligase, at 2,763,627 to 2,765,678 _RS20440 Position (kb) 2762 2763 2764Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2761.914 kb on + strandat 2762.051 kb on + strandat 2762.051 kb on + strandat 2762.051 kb on + strandat 2762.052 kb on - strandat 2762.052 kb on - strandat 2762.131 kb on + strandat 2762.131 kb on + strandat 2762.132 kb on - strandat 2762.944 kb on + strand, within RPSI07_RS20435at 2762.986 kb on + strand, within RPSI07_RS20435at 2762.986 kb on + strand, within RPSI07_RS20435at 2762.986 kb on + strand, within RPSI07_RS20435at 2762.986 kb on + strand, within RPSI07_RS20435at 2762.986 kb on + strand, within RPSI07_RS20435at 2762.987 kb on - strand, within RPSI07_RS20435at 2762.987 kb on - strand, within RPSI07_RS20435at 2762.987 kb on - strand, within RPSI07_RS20435at 2763.004 kb on + strand, within RPSI07_RS20435at 2763.355 kb on + strand, within RPSI07_RS20435at 2763.356 kb on - strand, within RPSI07_RS20435at 2763.361 kb on + strand, within RPSI07_RS20435at 2763.410 kb on - strandat 2763.410 kb on - strandat 2763.505 kb on + strandat 2763.506 kb on - strandat 2763.521 kb on - strandat 2763.536 kb on - strandat 2763.536 kb on - strandat 2763.591 kb on + strandat 2763.591 kb on + strandat 2763.591 kb on + strandat 2763.632 kb on + strandat 2763.633 kb on - strandat 2763.633 kb on - strandat 2763.655 kb on + strandat 2763.656 kb on - strandat 2763.656 kb on - strandat 2763.702 kb on + strandat 2763.825 kb on + strandat 2763.826 kb on - strandat 2763.918 kb on + strand, within RPSI07_RS20440at 2763.919 kb on - strand, within RPSI07_RS20440at 2764.254 kb on + strand, within RPSI07_RS20440at 2764.254 kb on + strand, within RPSI07_RS20440at 2764.255 kb on - strand, within RPSI07_RS20440at 2764.260 kb on + strand, within RPSI07_RS20440at 2764.337 kb on - strand, within RPSI07_RS20440at 2764.337 kb on - strand, within RPSI07_RS20440at 2764.470 kb on + strand, within RPSI07_RS20440at 2764.471 kb on - strand, within RPSI07_RS20440at 2764.507 kb on + strand, within RPSI07_RS20440at 2764.507 kb on + strand, within RPSI07_RS20440at 2764.507 kb on + strand, within RPSI07_RS20440at 2764.507 kb on + strand, within RPSI07_RS20440at 2764.507 kb on + strand, within RPSI07_RS20440at 2764.507 kb on + strand, within RPSI07_RS20440at 2764.507 kb on + strand, within RPSI07_RS20440at 2764.507 kb on + strand, within RPSI07_RS20440at 2764.507 kb on + strand, within RPSI07_RS20440at 2764.508 kb on - strand, within RPSI07_RS20440at 2764.508 kb on - strand, within RPSI07_RS20440at 2764.508 kb on - strand, within RPSI07_RS20440at 2764.508 kb on - strand, within RPSI07_RS20440at 2764.508 kb on - strand, within RPSI07_RS20440

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,761,914 + -1.2
2,762,051 + -0.5
2,762,051 + +1.1
2,762,051 + -0.3
2,762,052 - -1.4
2,762,052 - -0.3
2,762,131 + -0.2
2,762,131 + -0.3
2,762,132 - -1.2
2,762,944 + RPSI07_RS20435 0.59 -2.5
2,762,986 + RPSI07_RS20435 0.62 +0.8
2,762,986 + RPSI07_RS20435 0.62 -0.8
2,762,986 + RPSI07_RS20435 0.62 -1.3
2,762,986 + RPSI07_RS20435 0.62 +1.0
2,762,986 + RPSI07_RS20435 0.62 +1.4
2,762,987 - RPSI07_RS20435 0.62 -1.0
2,762,987 - RPSI07_RS20435 0.62 +0.9
2,762,987 - RPSI07_RS20435 0.62 +0.9
2,763,004 + RPSI07_RS20435 0.63 -0.4
2,763,355 + RPSI07_RS20435 0.87 +1.4
2,763,356 - RPSI07_RS20435 0.87 -0.2
2,763,361 + RPSI07_RS20435 0.87 -1.6
2,763,410 - +0.4
2,763,410 - +0.6
2,763,505 + -1.4
2,763,506 - -1.0
2,763,521 - -1.7
2,763,536 - +0.3
2,763,536 - -2.0
2,763,591 + +0.4
2,763,591 + -2.0
2,763,591 + +1.2
2,763,632 + +0.9
2,763,633 - +1.4
2,763,633 - -0.2
2,763,655 + -0.8
2,763,656 - +0.1
2,763,656 - -0.3
2,763,702 + +0.4
2,763,825 + -1.2
2,763,826 - -0.7
2,763,918 + RPSI07_RS20440 0.14 +0.7
2,763,919 - RPSI07_RS20440 0.14 +0.2
2,764,254 + RPSI07_RS20440 0.31 +1.6
2,764,254 + RPSI07_RS20440 0.31 +1.1
2,764,255 - RPSI07_RS20440 0.31 -0.4
2,764,260 + RPSI07_RS20440 0.31 -0.3
2,764,337 - RPSI07_RS20440 0.35 -0.7
2,764,337 - RPSI07_RS20440 0.35 -0.7
2,764,470 + RPSI07_RS20440 0.41 -2.5
2,764,471 - RPSI07_RS20440 0.41 +1.9
2,764,507 + RPSI07_RS20440 0.43 +0.3
2,764,507 + RPSI07_RS20440 0.43 +0.1
2,764,507 + RPSI07_RS20440 0.43 -0.0
2,764,507 + RPSI07_RS20440 0.43 -0.3
2,764,507 + RPSI07_RS20440 0.43 -3.0
2,764,507 + RPSI07_RS20440 0.43 -0.0
2,764,507 + RPSI07_RS20440 0.43 -0.7
2,764,507 + RPSI07_RS20440 0.43 -1.0
2,764,507 + RPSI07_RS20440 0.43 -1.7
2,764,508 - RPSI07_RS20440 0.43 +0.2
2,764,508 - RPSI07_RS20440 0.43 -0.2
2,764,508 - RPSI07_RS20440 0.43 -1.0
2,764,508 - RPSI07_RS20440 0.43 +1.8
2,764,508 - RPSI07_RS20440 0.43 +2.3

Or see this region's nucleotide sequence