Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS20430 and RPSI07_RS20435 are separated by 124 nucleotides RPSI07_RS20435 and RPSI07_RS20440 are separated by 68 nucleotides
RPSI07_RS20430: RPSI07_RS20430 - 4-hydroxy-3-polyprenylbenzoate decarboxylase, at 2,760,405 to 2,761,916
_RS20430
RPSI07_RS20435: RPSI07_RS20435 - NAD-dependent succinate-semialdehyde dehydrogenase, at 2,762,041 to 2,763,558
_RS20435
RPSI07_RS20440: RPSI07_RS20440 - acetoacetate--CoA ligase, at 2,763,627 to 2,765,678
_RS20440
Position (kb)
2762
2763
2764 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2761.914 kb on + strand at 2762.051 kb on + strand at 2762.051 kb on + strand at 2762.051 kb on + strand at 2762.052 kb on - strand at 2762.052 kb on - strand at 2762.131 kb on + strand at 2762.131 kb on + strand at 2762.132 kb on - strand at 2762.944 kb on + strand, within RPSI07_RS20435 at 2762.986 kb on + strand, within RPSI07_RS20435 at 2762.986 kb on + strand, within RPSI07_RS20435 at 2762.986 kb on + strand, within RPSI07_RS20435 at 2762.986 kb on + strand, within RPSI07_RS20435 at 2762.986 kb on + strand, within RPSI07_RS20435 at 2762.987 kb on - strand, within RPSI07_RS20435 at 2762.987 kb on - strand, within RPSI07_RS20435 at 2762.987 kb on - strand, within RPSI07_RS20435 at 2763.004 kb on + strand, within RPSI07_RS20435 at 2763.355 kb on + strand, within RPSI07_RS20435 at 2763.356 kb on - strand, within RPSI07_RS20435 at 2763.361 kb on + strand, within RPSI07_RS20435 at 2763.410 kb on - strand at 2763.410 kb on - strand at 2763.505 kb on + strand at 2763.506 kb on - strand at 2763.521 kb on - strand at 2763.536 kb on - strand at 2763.536 kb on - strand at 2763.591 kb on + strand at 2763.591 kb on + strand at 2763.591 kb on + strand at 2763.632 kb on + strand at 2763.633 kb on - strand at 2763.633 kb on - strand at 2763.655 kb on + strand at 2763.656 kb on - strand at 2763.656 kb on - strand at 2763.702 kb on + strand at 2763.825 kb on + strand at 2763.826 kb on - strand at 2763.918 kb on + strand, within RPSI07_RS20440 at 2763.919 kb on - strand, within RPSI07_RS20440 at 2764.254 kb on + strand, within RPSI07_RS20440 at 2764.254 kb on + strand, within RPSI07_RS20440 at 2764.255 kb on - strand, within RPSI07_RS20440 at 2764.260 kb on + strand, within RPSI07_RS20440 at 2764.337 kb on - strand, within RPSI07_RS20440 at 2764.337 kb on - strand, within RPSI07_RS20440 at 2764.470 kb on + strand, within RPSI07_RS20440 at 2764.471 kb on - strand, within RPSI07_RS20440 at 2764.507 kb on + strand, within RPSI07_RS20440 at 2764.507 kb on + strand, within RPSI07_RS20440 at 2764.507 kb on + strand, within RPSI07_RS20440 at 2764.507 kb on + strand, within RPSI07_RS20440 at 2764.507 kb on + strand, within RPSI07_RS20440 at 2764.507 kb on + strand, within RPSI07_RS20440 at 2764.507 kb on + strand, within RPSI07_RS20440 at 2764.507 kb on + strand, within RPSI07_RS20440 at 2764.507 kb on + strand, within RPSI07_RS20440 at 2764.508 kb on - strand, within RPSI07_RS20440 at 2764.508 kb on - strand, within RPSI07_RS20440 at 2764.508 kb on - strand, within RPSI07_RS20440 at 2764.508 kb on - strand, within RPSI07_RS20440 at 2764.508 kb on - strand, within RPSI07_RS20440
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,761,914 + -1.2 2,762,051 + -0.5 2,762,051 + +1.1 2,762,051 + -0.3 2,762,052 - -1.4 2,762,052 - -0.3 2,762,131 + -0.2 2,762,131 + -0.3 2,762,132 - -1.2 2,762,944 + RPSI07_RS20435 0.59 -2.5 2,762,986 + RPSI07_RS20435 0.62 +0.8 2,762,986 + RPSI07_RS20435 0.62 -0.8 2,762,986 + RPSI07_RS20435 0.62 -1.3 2,762,986 + RPSI07_RS20435 0.62 +1.0 2,762,986 + RPSI07_RS20435 0.62 +1.4 2,762,987 - RPSI07_RS20435 0.62 -1.0 2,762,987 - RPSI07_RS20435 0.62 +0.9 2,762,987 - RPSI07_RS20435 0.62 +0.9 2,763,004 + RPSI07_RS20435 0.63 -0.4 2,763,355 + RPSI07_RS20435 0.87 +1.4 2,763,356 - RPSI07_RS20435 0.87 -0.2 2,763,361 + RPSI07_RS20435 0.87 -1.6 2,763,410 - +0.4 2,763,410 - +0.6 2,763,505 + -1.4 2,763,506 - -1.0 2,763,521 - -1.7 2,763,536 - +0.3 2,763,536 - -2.0 2,763,591 + +0.4 2,763,591 + -2.0 2,763,591 + +1.2 2,763,632 + +0.9 2,763,633 - +1.4 2,763,633 - -0.2 2,763,655 + -0.8 2,763,656 - +0.1 2,763,656 - -0.3 2,763,702 + +0.4 2,763,825 + -1.2 2,763,826 - -0.7 2,763,918 + RPSI07_RS20440 0.14 +0.7 2,763,919 - RPSI07_RS20440 0.14 +0.2 2,764,254 + RPSI07_RS20440 0.31 +1.6 2,764,254 + RPSI07_RS20440 0.31 +1.1 2,764,255 - RPSI07_RS20440 0.31 -0.4 2,764,260 + RPSI07_RS20440 0.31 -0.3 2,764,337 - RPSI07_RS20440 0.35 -0.7 2,764,337 - RPSI07_RS20440 0.35 -0.7 2,764,470 + RPSI07_RS20440 0.41 -2.5 2,764,471 - RPSI07_RS20440 0.41 +1.9 2,764,507 + RPSI07_RS20440 0.43 +0.3 2,764,507 + RPSI07_RS20440 0.43 +0.1 2,764,507 + RPSI07_RS20440 0.43 -0.0 2,764,507 + RPSI07_RS20440 0.43 -0.3 2,764,507 + RPSI07_RS20440 0.43 -3.0 2,764,507 + RPSI07_RS20440 0.43 -0.0 2,764,507 + RPSI07_RS20440 0.43 -0.7 2,764,507 + RPSI07_RS20440 0.43 -1.0 2,764,507 + RPSI07_RS20440 0.43 -1.7 2,764,508 - RPSI07_RS20440 0.43 +0.2 2,764,508 - RPSI07_RS20440 0.43 -0.2 2,764,508 - RPSI07_RS20440 0.43 -1.0 2,764,508 - RPSI07_RS20440 0.43 +1.8 2,764,508 - RPSI07_RS20440 0.43 +2.3
Or see this region's nucleotide sequence