Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS20015 and RPSI07_RS20020 are separated by 33 nucleotides RPSI07_RS20020 and RPSI07_RS20025 are separated by 224 nucleotides RPSI07_RS20025 and RPSI07_RS20030 are separated by 10 nucleotides
RPSI07_RS20015: RPSI07_RS20015 - CoA transferase, at 2,676,905 to 2,678,125
_RS20015
RPSI07_RS20020: RPSI07_RS20020 - DUF2214 domain-containing protein, at 2,678,159 to 2,678,611
_RS20020
RPSI07_RS20025: RPSI07_RS20025 - tripartite tricarboxylate transporter TctB family protein, at 2,678,836 to 2,679,321
_RS20025
RPSI07_RS20030: RPSI07_RS20030 - tripartite tricarboxylate transporter permease, at 2,679,332 to 2,680,834
_RS20030
Position (kb)
2678
2679 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2677.211 kb on + strand, within RPSI07_RS20015 at 2677.211 kb on + strand, within RPSI07_RS20015 at 2677.212 kb on - strand, within RPSI07_RS20015 at 2677.212 kb on - strand, within RPSI07_RS20015 at 2677.212 kb on - strand, within RPSI07_RS20015 at 2677.235 kb on + strand, within RPSI07_RS20015 at 2677.236 kb on - strand, within RPSI07_RS20015 at 2677.237 kb on - strand, within RPSI07_RS20015 at 2677.284 kb on - strand, within RPSI07_RS20015 at 2677.457 kb on + strand, within RPSI07_RS20015 at 2677.457 kb on + strand, within RPSI07_RS20015 at 2677.457 kb on + strand, within RPSI07_RS20015 at 2677.457 kb on + strand, within RPSI07_RS20015 at 2677.458 kb on - strand, within RPSI07_RS20015 at 2677.458 kb on - strand, within RPSI07_RS20015 at 2677.458 kb on - strand, within RPSI07_RS20015 at 2677.517 kb on + strand, within RPSI07_RS20015 at 2677.517 kb on + strand, within RPSI07_RS20015 at 2677.518 kb on - strand, within RPSI07_RS20015 at 2677.577 kb on + strand, within RPSI07_RS20015 at 2677.577 kb on + strand, within RPSI07_RS20015 at 2677.577 kb on + strand, within RPSI07_RS20015 at 2677.577 kb on + strand, within RPSI07_RS20015 at 2677.578 kb on - strand, within RPSI07_RS20015 at 2677.637 kb on + strand, within RPSI07_RS20015 at 2677.638 kb on - strand, within RPSI07_RS20015 at 2678.123 kb on + strand at 2678.123 kb on + strand at 2678.183 kb on + strand at 2678.183 kb on + strand at 2678.183 kb on + strand at 2678.184 kb on - strand at 2678.192 kb on + strand at 2678.192 kb on + strand at 2678.192 kb on + strand at 2678.192 kb on + strand at 2678.193 kb on - strand at 2678.193 kb on - strand at 2678.193 kb on - strand at 2678.193 kb on - strand at 2678.193 kb on - strand at 2678.289 kb on - strand, within RPSI07_RS20020 at 2678.300 kb on + strand, within RPSI07_RS20020 at 2678.300 kb on + strand, within RPSI07_RS20020 at 2678.375 kb on + strand, within RPSI07_RS20020 at 2678.375 kb on + strand, within RPSI07_RS20020 at 2678.376 kb on - strand, within RPSI07_RS20020 at 2678.376 kb on - strand, within RPSI07_RS20020 at 2678.376 kb on - strand, within RPSI07_RS20020 at 2678.761 kb on + strand at 2678.761 kb on + strand at 2678.762 kb on - strand at 2678.980 kb on + strand, within RPSI07_RS20025 at 2679.168 kb on - strand, within RPSI07_RS20025 at 2679.271 kb on + strand, within RPSI07_RS20025 at 2679.271 kb on + strand, within RPSI07_RS20025 at 2679.272 kb on - strand, within RPSI07_RS20025 at 2679.395 kb on + strand at 2679.395 kb on + strand at 2679.396 kb on - strand at 2679.396 kb on - strand at 2679.488 kb on + strand, within RPSI07_RS20030 at 2679.488 kb on + strand, within RPSI07_RS20030 at 2679.489 kb on - strand, within RPSI07_RS20030 at 2679.489 kb on - strand, within RPSI07_RS20030 at 2679.489 kb on - strand, within RPSI07_RS20030 at 2679.489 kb on - strand, within RPSI07_RS20030 at 2679.489 kb on - strand, within RPSI07_RS20030 at 2679.533 kb on + strand, within RPSI07_RS20030 at 2679.533 kb on + strand, within RPSI07_RS20030 at 2679.533 kb on + strand, within RPSI07_RS20030 at 2679.533 kb on + strand, within RPSI07_RS20030 at 2679.534 kb on - strand, within RPSI07_RS20030 at 2679.534 kb on - strand, within RPSI07_RS20030 at 2679.534 kb on - strand, within RPSI07_RS20030 at 2679.534 kb on - strand, within RPSI07_RS20030 at 2679.534 kb on - strand, within RPSI07_RS20030 at 2679.534 kb on - strand, within RPSI07_RS20030 at 2679.534 kb on - strand, within RPSI07_RS20030 at 2679.534 kb on - strand, within RPSI07_RS20030 at 2679.536 kb on + strand, within RPSI07_RS20030 at 2679.537 kb on - strand, within RPSI07_RS20030 at 2679.548 kb on + strand, within RPSI07_RS20030 at 2679.549 kb on - strand, within RPSI07_RS20030
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,677,211 + RPSI07_RS20015 0.25 +1.4 2,677,211 + RPSI07_RS20015 0.25 -0.6 2,677,212 - RPSI07_RS20015 0.25 -0.5 2,677,212 - RPSI07_RS20015 0.25 -0.8 2,677,212 - RPSI07_RS20015 0.25 +0.5 2,677,235 + RPSI07_RS20015 0.27 +0.1 2,677,236 - RPSI07_RS20015 0.27 -1.6 2,677,237 - RPSI07_RS20015 0.27 +0.1 2,677,284 - RPSI07_RS20015 0.31 -0.2 2,677,457 + RPSI07_RS20015 0.45 -0.9 2,677,457 + RPSI07_RS20015 0.45 -1.4 2,677,457 + RPSI07_RS20015 0.45 -0.2 2,677,457 + RPSI07_RS20015 0.45 -0.5 2,677,458 - RPSI07_RS20015 0.45 -0.3 2,677,458 - RPSI07_RS20015 0.45 -0.9 2,677,458 - RPSI07_RS20015 0.45 -0.0 2,677,517 + RPSI07_RS20015 0.50 +0.6 2,677,517 + RPSI07_RS20015 0.50 -1.3 2,677,518 - RPSI07_RS20015 0.50 -2.4 2,677,577 + RPSI07_RS20015 0.55 +1.4 2,677,577 + RPSI07_RS20015 0.55 +0.2 2,677,577 + RPSI07_RS20015 0.55 +1.9 2,677,577 + RPSI07_RS20015 0.55 -0.0 2,677,578 - RPSI07_RS20015 0.55 -0.7 2,677,637 + RPSI07_RS20015 0.60 +0.8 2,677,638 - RPSI07_RS20015 0.60 +0.2 2,678,123 + +0.8 2,678,123 + +0.3 2,678,183 + +0.4 2,678,183 + +0.4 2,678,183 + +0.1 2,678,184 - -0.9 2,678,192 + -0.1 2,678,192 + -0.5 2,678,192 + -0.4 2,678,192 + -1.7 2,678,193 - +0.0 2,678,193 - +2.1 2,678,193 - -1.2 2,678,193 - +1.9 2,678,193 - -0.1 2,678,289 - RPSI07_RS20020 0.29 -0.1 2,678,300 + RPSI07_RS20020 0.31 -0.4 2,678,300 + RPSI07_RS20020 0.31 -2.3 2,678,375 + RPSI07_RS20020 0.48 -1.5 2,678,375 + RPSI07_RS20020 0.48 -2.1 2,678,376 - RPSI07_RS20020 0.48 -0.7 2,678,376 - RPSI07_RS20020 0.48 -1.2 2,678,376 - RPSI07_RS20020 0.48 -2.1 2,678,761 + +0.0 2,678,761 + -1.0 2,678,762 - +0.2 2,678,980 + RPSI07_RS20025 0.30 +0.4 2,679,168 - RPSI07_RS20025 0.68 -1.4 2,679,271 + RPSI07_RS20025 0.90 -0.4 2,679,271 + RPSI07_RS20025 0.90 -0.2 2,679,272 - RPSI07_RS20025 0.90 -0.3 2,679,395 + +0.1 2,679,395 + +0.5 2,679,396 - +1.4 2,679,396 - +2.9 2,679,488 + RPSI07_RS20030 0.10 -2.4 2,679,488 + RPSI07_RS20030 0.10 +4.5 2,679,489 - RPSI07_RS20030 0.10 +2.4 2,679,489 - RPSI07_RS20030 0.10 +2.4 2,679,489 - RPSI07_RS20030 0.10 +2.4 2,679,489 - RPSI07_RS20030 0.10 +0.4 2,679,489 - RPSI07_RS20030 0.10 -0.7 2,679,533 + RPSI07_RS20030 0.13 +0.0 2,679,533 + RPSI07_RS20030 0.13 +1.3 2,679,533 + RPSI07_RS20030 0.13 -0.5 2,679,533 + RPSI07_RS20030 0.13 -0.2 2,679,534 - RPSI07_RS20030 0.13 +0.5 2,679,534 - RPSI07_RS20030 0.13 -0.1 2,679,534 - RPSI07_RS20030 0.13 +0.4 2,679,534 - RPSI07_RS20030 0.13 +1.9 2,679,534 - RPSI07_RS20030 0.13 -2.0 2,679,534 - RPSI07_RS20030 0.13 +0.5 2,679,534 - RPSI07_RS20030 0.13 +3.4 2,679,534 - RPSI07_RS20030 0.13 +0.9 2,679,536 + RPSI07_RS20030 0.14 +1.3 2,679,537 - RPSI07_RS20030 0.14 -2.2 2,679,548 + RPSI07_RS20030 0.14 -0.7 2,679,549 - RPSI07_RS20030 0.14 +1.0
Or see this region's nucleotide sequence