Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13640 and RPSI07_RS13645 are separated by 31 nucleotides RPSI07_RS13645 and RPSI07_RS13650 are separated by 147 nucleotides RPSI07_RS13650 and RPSI07_RS13655 are separated by 138 nucleotides RPSI07_RS13655 and RPSI07_RS13660 are separated by 2 nucleotides
RPSI07_RS13640: RPSI07_RS13640 - alkylhydroperoxidase, at 1,253,177 to 1,253,782
_RS13640
RPSI07_RS13645: RPSI07_RS13645 - hypothetical protein, at 1,253,814 to 1,254,104
_RS13645
RPSI07_RS13650: RPSI07_RS13650 - ribonuclease G, at 1,254,252 to 1,255,721
_RS13650
RPSI07_RS13655: RPSI07_RS13655 - septum formation inhibitor Maf, at 1,255,860 to 1,256,477
_RS13655
RPSI07_RS13660: RPSI07_RS13660 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,256,480 to 1,256,950
_RS13660
Position (kb) 1254
1255
1256 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1253.454 kb on - strand, within RPSI07_RS13640 at 1253.532 kb on - strand, within RPSI07_RS13640 at 1253.737 kb on + strand at 1253.750 kb on + strand at 1253.750 kb on + strand at 1253.750 kb on + strand at 1254.013 kb on + strand, within RPSI07_RS13645 at 1254.014 kb on - strand, within RPSI07_RS13645 at 1254.070 kb on + strand, within RPSI07_RS13645 at 1255.831 kb on + strand at 1255.862 kb on - strand at 1255.930 kb on + strand, within RPSI07_RS13655 at 1256.020 kb on + strand, within RPSI07_RS13655 at 1256.020 kb on + strand, within RPSI07_RS13655 at 1256.314 kb on + strand, within RPSI07_RS13655 at 1256.315 kb on - strand, within RPSI07_RS13655 at 1256.315 kb on - strand, within RPSI07_RS13655 at 1256.449 kb on + strand at 1256.450 kb on - strand at 1256.450 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,253,454 - RPSI07_RS13640 0.46 -0.2 1,253,532 - RPSI07_RS13640 0.59 -0.3 1,253,737 + +1.3 1,253,750 + +1.3 1,253,750 + +0.9 1,253,750 + +0.3 1,254,013 + RPSI07_RS13645 0.68 +1.1 1,254,014 - RPSI07_RS13645 0.69 -0.8 1,254,070 + RPSI07_RS13645 0.88 +2.0 1,255,831 + -0.7 1,255,862 - +0.6 1,255,930 + RPSI07_RS13655 0.11 -2.3 1,256,020 + RPSI07_RS13655 0.26 -0.6 1,256,020 + RPSI07_RS13655 0.26 +1.8 1,256,314 + RPSI07_RS13655 0.73 -0.5 1,256,315 - RPSI07_RS13655 0.74 -1.3 1,256,315 - RPSI07_RS13655 0.74 -0.1 1,256,449 + +1.3 1,256,450 - -1.9 1,256,450 - +0.4
Or see this region's nucleotide sequence