Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS13480

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS13475 and RPSI07_RS13480 are separated by 4 nucleotidesRPSI07_RS13480 and RPSI07_RS13485 are separated by 170 nucleotides RPSI07_RS13475: RPSI07_RS13475 - TIGR00730 family Rossman fold protein, at 1,212,487 to 1,213,449 _RS13475 RPSI07_RS13480: RPSI07_RS13480 - DNA polymerase I, at 1,213,454 to 1,216,288 _RS13480 RPSI07_RS13485: RPSI07_RS13485 - NAD(P)/FAD-dependent oxidoreductase, at 1,216,459 to 1,217,784 _RS13485 Position (kb) 1213 1214 1215 1216 1217Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1212.488 kb on + strandat 1212.488 kb on + strandat 1212.488 kb on + strandat 1212.495 kb on - strandat 1212.545 kb on + strandat 1212.555 kb on - strandat 1213.025 kb on + strand, within RPSI07_RS13475at 1213.026 kb on - strand, within RPSI07_RS13475at 1213.094 kb on + strand, within RPSI07_RS13475at 1213.156 kb on - strand, within RPSI07_RS13475at 1214.381 kb on + strand, within RPSI07_RS13480at 1214.381 kb on + strand, within RPSI07_RS13480at 1214.453 kb on + strand, within RPSI07_RS13480at 1214.819 kb on + strand, within RPSI07_RS13480at 1214.820 kb on - strand, within RPSI07_RS13480at 1214.820 kb on - strand, within RPSI07_RS13480at 1214.894 kb on + strand, within RPSI07_RS13480at 1214.966 kb on + strand, within RPSI07_RS13480at 1215.014 kb on + strand, within RPSI07_RS13480at 1215.339 kb on - strand, within RPSI07_RS13480at 1216.453 kb on - strandat 1216.500 kb on - strandat 1216.816 kb on + strand, within RPSI07_RS13485at 1216.817 kb on - strand, within RPSI07_RS13485at 1217.071 kb on + strandat 1217.072 kb on - strand, within RPSI07_RS13485

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,212,488 + +1.3
1,212,488 + +0.5
1,212,488 + +0.7
1,212,495 - +1.0
1,212,545 + -0.3
1,212,555 - -0.5
1,213,025 + RPSI07_RS13475 0.56 +1.1
1,213,026 - RPSI07_RS13475 0.56 -1.5
1,213,094 + RPSI07_RS13475 0.63 -1.2
1,213,156 - RPSI07_RS13475 0.69 -1.7
1,214,381 + RPSI07_RS13480 0.33 -2.7
1,214,381 + RPSI07_RS13480 0.33 +0.3
1,214,453 + RPSI07_RS13480 0.35 -2.2
1,214,819 + RPSI07_RS13480 0.48 +3.3
1,214,820 - RPSI07_RS13480 0.48 -0.7
1,214,820 - RPSI07_RS13480 0.48 -1.0
1,214,894 + RPSI07_RS13480 0.51 -2.8
1,214,966 + RPSI07_RS13480 0.53 -2.4
1,215,014 + RPSI07_RS13480 0.55 -1.3
1,215,339 - RPSI07_RS13480 0.66 -1.1
1,216,453 - -0.2
1,216,500 - -0.1
1,216,816 + RPSI07_RS13485 0.27 -1.2
1,216,817 - RPSI07_RS13485 0.27 -0.8
1,217,071 + +1.3
1,217,072 - RPSI07_RS13485 0.46 +1.4

Or see this region's nucleotide sequence