Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10760 and RPSI07_RS10765 overlap by 4 nucleotides RPSI07_RS10765 and RPSI07_RS10770 are separated by 134 nucleotides RPSI07_RS10770 and RPSI07_RS10775 are separated by 0 nucleotides RPSI07_RS10775 and RPSI07_RS10780 are separated by 72 nucleotides
RPSI07_RS10760: RPSI07_RS10760 - phosphoglycolate phosphatase, at 613,235 to 613,975
_RS10760
RPSI07_RS10765: RPSI07_RS10765 - ribulose-phosphate 3-epimerase, at 613,972 to 614,700
_RS10765
RPSI07_RS10770: RPSI07_RS10770 - Co2+/Mg2+ efflux protein ApaG, at 614,835 to 615,209
_RS10770
RPSI07_RS10775: RPSI07_RS10775 - hypothetical protein, at 615,210 to 615,461
_RS10775
RPSI07_RS10780: RPSI07_RS10780 - murein transglycosylase, at 615,534 to 616,676
_RS10780
Position (kb) 614
615
616 Strain fitness (log2 ratio) -2
-1
0
1
2 at 614.745 kb on - strand at 614.790 kb on - strand at 614.834 kb on + strand at 614.834 kb on + strand at 614.834 kb on + strand at 614.835 kb on - strand at 614.835 kb on - strand at 614.844 kb on + strand at 614.874 kb on + strand, within RPSI07_RS10770 at 614.875 kb on - strand, within RPSI07_RS10770 at 614.942 kb on + strand, within RPSI07_RS10770 at 615.075 kb on + strand, within RPSI07_RS10770 at 615.075 kb on + strand, within RPSI07_RS10770 at 615.075 kb on + strand, within RPSI07_RS10770 at 615.076 kb on - strand, within RPSI07_RS10770 at 615.076 kb on - strand, within RPSI07_RS10770 at 615.126 kb on + strand, within RPSI07_RS10770 at 615.494 kb on + strand at 615.495 kb on - strand at 615.945 kb on + strand, within RPSI07_RS10780 at 615.949 kb on - strand, within RPSI07_RS10780 at 615.949 kb on - strand, within RPSI07_RS10780 at 615.991 kb on - strand, within RPSI07_RS10780 at 616.196 kb on + strand, within RPSI07_RS10780 at 616.199 kb on + strand, within RPSI07_RS10780
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 614,745 - -0.2 614,790 - +1.4 614,834 + -1.2 614,834 + -0.2 614,834 + -2.4 614,835 - +1.4 614,835 - -0.0 614,844 + +0.4 614,874 + RPSI07_RS10770 0.10 +0.5 614,875 - RPSI07_RS10770 0.11 +2.2 614,942 + RPSI07_RS10770 0.29 -0.1 615,075 + RPSI07_RS10770 0.64 +1.4 615,075 + RPSI07_RS10770 0.64 +1.4 615,075 + RPSI07_RS10770 0.64 +1.9 615,076 - RPSI07_RS10770 0.64 +0.4 615,076 - RPSI07_RS10770 0.64 -1.1 615,126 + RPSI07_RS10770 0.78 -0.1 615,494 + -1.8 615,495 - -0.6 615,945 + RPSI07_RS10780 0.36 +0.4 615,949 - RPSI07_RS10780 0.36 +0.4 615,949 - RPSI07_RS10780 0.36 -1.1 615,991 - RPSI07_RS10780 0.40 +0.4 616,196 + RPSI07_RS10780 0.58 +2.4 616,199 + RPSI07_RS10780 0.58 +0.6
Or see this region's nucleotide sequence