Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07440 and RPSI07_RS07445 are separated by 463 nucleotides RPSI07_RS07445 and RPSI07_RS07450 are separated by 72 nucleotides
RPSI07_RS07440: RPSI07_RS07440 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,970,851 to 1,971,993
_RS07440
RPSI07_RS07445: RPSI07_RS07445 - LysR family transcriptional regulator, at 1,972,457 to 1,973,347
_RS07445
RPSI07_RS07450: RPSI07_RS07450 - porin, at 1,973,420 to 1,974,547
_RS07450
Position (kb) 1972
1973
1974 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1971.632 kb on + strand, within RPSI07_RS07440 at 1971.632 kb on + strand, within RPSI07_RS07440 at 1971.632 kb on + strand, within RPSI07_RS07440 at 1971.632 kb on + strand, within RPSI07_RS07440 at 1971.632 kb on + strand, within RPSI07_RS07440 at 1971.632 kb on + strand, within RPSI07_RS07440 at 1971.632 kb on + strand, within RPSI07_RS07440 at 1971.632 kb on + strand, within RPSI07_RS07440 at 1971.633 kb on - strand, within RPSI07_RS07440 at 1971.633 kb on - strand, within RPSI07_RS07440 at 1971.667 kb on + strand, within RPSI07_RS07440 at 1971.804 kb on + strand, within RPSI07_RS07440 at 1971.804 kb on + strand, within RPSI07_RS07440 at 1971.804 kb on + strand, within RPSI07_RS07440 at 1971.805 kb on - strand, within RPSI07_RS07440 at 1971.805 kb on - strand, within RPSI07_RS07440 at 1971.805 kb on - strand, within RPSI07_RS07440 at 1971.805 kb on - strand, within RPSI07_RS07440 at 1971.805 kb on - strand, within RPSI07_RS07440 at 1971.805 kb on - strand, within RPSI07_RS07440 at 1971.805 kb on - strand, within RPSI07_RS07440 at 1971.805 kb on - strand, within RPSI07_RS07440 at 1972.235 kb on + strand at 1972.236 kb on - strand at 1972.237 kb on + strand at 1972.237 kb on + strand at 1972.237 kb on + strand at 1972.237 kb on + strand at 1972.238 kb on - strand at 1972.296 kb on + strand at 1972.296 kb on + strand at 1972.296 kb on + strand at 1972.296 kb on + strand at 1972.297 kb on - strand at 1972.297 kb on - strand at 1972.368 kb on + strand at 1972.368 kb on + strand at 1972.369 kb on - strand at 1972.396 kb on + strand at 1972.397 kb on - strand at 1972.397 kb on - strand at 1972.470 kb on + strand at 1972.470 kb on + strand at 1972.470 kb on + strand at 1972.470 kb on + strand at 1972.470 kb on + strand at 1972.470 kb on + strand at 1972.470 kb on + strand at 1972.470 kb on + strand at 1972.471 kb on - strand at 1972.471 kb on - strand at 1972.471 kb on - strand at 1972.661 kb on + strand, within RPSI07_RS07445 at 1972.661 kb on + strand, within RPSI07_RS07445 at 1972.661 kb on + strand, within RPSI07_RS07445 at 1972.661 kb on + strand, within RPSI07_RS07445 at 1972.661 kb on + strand, within RPSI07_RS07445 at 1972.662 kb on - strand, within RPSI07_RS07445 at 1972.662 kb on - strand, within RPSI07_RS07445 at 1972.739 kb on + strand, within RPSI07_RS07445 at 1972.739 kb on + strand, within RPSI07_RS07445 at 1972.739 kb on + strand, within RPSI07_RS07445 at 1972.740 kb on - strand, within RPSI07_RS07445 at 1972.740 kb on - strand, within RPSI07_RS07445 at 1972.740 kb on - strand, within RPSI07_RS07445 at 1972.880 kb on + strand, within RPSI07_RS07445 at 1972.880 kb on + strand, within RPSI07_RS07445 at 1972.880 kb on + strand, within RPSI07_RS07445 at 1972.880 kb on + strand, within RPSI07_RS07445 at 1972.880 kb on + strand, within RPSI07_RS07445 at 1972.880 kb on + strand, within RPSI07_RS07445 at 1972.880 kb on + strand, within RPSI07_RS07445 at 1972.881 kb on - strand, within RPSI07_RS07445 at 1972.881 kb on - strand, within RPSI07_RS07445 at 1972.881 kb on - strand, within RPSI07_RS07445 at 1972.881 kb on - strand, within RPSI07_RS07445 at 1972.881 kb on - strand, within RPSI07_RS07445 at 1972.895 kb on + strand, within RPSI07_RS07445 at 1972.895 kb on + strand, within RPSI07_RS07445 at 1972.895 kb on + strand, within RPSI07_RS07445 at 1972.895 kb on + strand, within RPSI07_RS07445 at 1972.896 kb on - strand, within RPSI07_RS07445 at 1972.896 kb on - strand, within RPSI07_RS07445 at 1972.896 kb on - strand, within RPSI07_RS07445 at 1972.979 kb on + strand, within RPSI07_RS07445 at 1972.980 kb on - strand, within RPSI07_RS07445 at 1973.072 kb on + strand, within RPSI07_RS07445 at 1973.072 kb on + strand, within RPSI07_RS07445 at 1973.072 kb on + strand, within RPSI07_RS07445 at 1973.094 kb on - strand, within RPSI07_RS07445 at 1973.124 kb on - strand, within RPSI07_RS07445 at 1973.264 kb on + strand at 1973.264 kb on + strand at 1973.264 kb on + strand at 1973.265 kb on - strand at 1973.267 kb on + strand at 1973.267 kb on + strand at 1973.268 kb on - strand at 1973.268 kb on - strand at 1973.268 kb on - strand at 1973.421 kb on + strand at 1973.421 kb on + strand at 1973.421 kb on + strand at 1973.421 kb on + strand at 1973.421 kb on + strand at 1973.422 kb on - strand at 1973.422 kb on - strand at 1973.482 kb on + strand at 1973.508 kb on + strand at 1973.508 kb on + strand at 1973.508 kb on + strand at 1973.508 kb on + strand at 1973.508 kb on + strand at 1973.509 kb on - strand at 1973.509 kb on - strand at 1973.509 kb on - strand at 1973.536 kb on - strand, within RPSI07_RS07450 at 1973.550 kb on + strand, within RPSI07_RS07450 at 1973.550 kb on + strand, within RPSI07_RS07450 at 1973.550 kb on + strand, within RPSI07_RS07450 at 1973.551 kb on - strand, within RPSI07_RS07450 at 1973.551 kb on - strand, within RPSI07_RS07450 at 1973.577 kb on + strand, within RPSI07_RS07450 at 1973.577 kb on + strand, within RPSI07_RS07450 at 1973.577 kb on + strand, within RPSI07_RS07450 at 1973.578 kb on - strand, within RPSI07_RS07450 at 1973.578 kb on - strand, within RPSI07_RS07450 at 1973.578 kb on - strand, within RPSI07_RS07450 at 1973.676 kb on + strand, within RPSI07_RS07450 at 1973.676 kb on + strand, within RPSI07_RS07450 at 1973.677 kb on - strand, within RPSI07_RS07450 at 1973.694 kb on + strand, within RPSI07_RS07450 at 1973.694 kb on + strand, within RPSI07_RS07450 at 1973.694 kb on + strand, within RPSI07_RS07450 at 1973.695 kb on - strand, within RPSI07_RS07450 at 1973.695 kb on - strand, within RPSI07_RS07450 at 1973.892 kb on + strand, within RPSI07_RS07450 at 1973.892 kb on + strand, within RPSI07_RS07450 at 1973.925 kb on + strand, within RPSI07_RS07450 at 1973.926 kb on - strand, within RPSI07_RS07450 at 1973.926 kb on - strand, within RPSI07_RS07450 at 1973.926 kb on - strand, within RPSI07_RS07450 at 1974.034 kb on - strand, within RPSI07_RS07450 at 1974.072 kb on + strand, within RPSI07_RS07450 at 1974.072 kb on + strand, within RPSI07_RS07450 at 1974.073 kb on - strand, within RPSI07_RS07450 at 1974.073 kb on - strand, within RPSI07_RS07450 at 1974.108 kb on + strand, within RPSI07_RS07450 at 1974.108 kb on + strand, within RPSI07_RS07450 at 1974.108 kb on + strand, within RPSI07_RS07450 at 1974.108 kb on + strand, within RPSI07_RS07450 at 1974.108 kb on + strand, within RPSI07_RS07450 at 1974.108 kb on + strand, within RPSI07_RS07450 at 1974.108 kb on + strand, within RPSI07_RS07450 at 1974.108 kb on + strand, within RPSI07_RS07450 at 1974.108 kb on + strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.109 kb on - strand, within RPSI07_RS07450 at 1974.112 kb on + strand, within RPSI07_RS07450 at 1974.113 kb on - strand, within RPSI07_RS07450 at 1974.133 kb on - strand, within RPSI07_RS07450 at 1974.186 kb on + strand, within RPSI07_RS07450 at 1974.187 kb on - strand, within RPSI07_RS07450
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,971,632 + RPSI07_RS07440 0.68 +1.4 1,971,632 + RPSI07_RS07440 0.68 -1.9 1,971,632 + RPSI07_RS07440 0.68 +1.4 1,971,632 + RPSI07_RS07440 0.68 +0.5 1,971,632 + RPSI07_RS07440 0.68 -1.2 1,971,632 + RPSI07_RS07440 0.68 -1.5 1,971,632 + RPSI07_RS07440 0.68 -0.7 1,971,632 + RPSI07_RS07440 0.68 +0.4 1,971,633 - RPSI07_RS07440 0.68 +2.8 1,971,633 - RPSI07_RS07440 0.68 -0.3 1,971,667 + RPSI07_RS07440 0.71 -0.8 1,971,804 + RPSI07_RS07440 0.83 +1.2 1,971,804 + RPSI07_RS07440 0.83 -0.3 1,971,804 + RPSI07_RS07440 0.83 -0.8 1,971,805 - RPSI07_RS07440 0.83 -1.5 1,971,805 - RPSI07_RS07440 0.83 -1.0 1,971,805 - RPSI07_RS07440 0.83 +0.7 1,971,805 - RPSI07_RS07440 0.83 -0.6 1,971,805 - RPSI07_RS07440 0.83 +0.2 1,971,805 - RPSI07_RS07440 0.83 -2.2 1,971,805 - RPSI07_RS07440 0.83 -0.4 1,971,805 - RPSI07_RS07440 0.83 -0.9 1,972,235 + -2.5 1,972,236 - -1.7 1,972,237 + +0.0 1,972,237 + -0.2 1,972,237 + +2.8 1,972,237 + +1.1 1,972,238 - -1.8 1,972,296 + +0.3 1,972,296 + -0.3 1,972,296 + +0.2 1,972,296 + +0.1 1,972,297 - -1.2 1,972,297 - +2.1 1,972,368 + +0.3 1,972,368 + +3.1 1,972,369 - -1.1 1,972,396 + +0.1 1,972,397 - -0.2 1,972,397 - -0.8 1,972,470 + -0.1 1,972,470 + -1.1 1,972,470 + +0.5 1,972,470 + +0.9 1,972,470 + -0.5 1,972,470 + +0.7 1,972,470 + +0.4 1,972,470 + +0.3 1,972,471 - -0.2 1,972,471 - +0.8 1,972,471 - -1.2 1,972,661 + RPSI07_RS07445 0.23 -0.8 1,972,661 + RPSI07_RS07445 0.23 -1.4 1,972,661 + RPSI07_RS07445 0.23 +0.2 1,972,661 + RPSI07_RS07445 0.23 -0.2 1,972,661 + RPSI07_RS07445 0.23 +2.2 1,972,662 - RPSI07_RS07445 0.23 +0.8 1,972,662 - RPSI07_RS07445 0.23 +1.1 1,972,739 + RPSI07_RS07445 0.32 +2.2 1,972,739 + RPSI07_RS07445 0.32 +0.3 1,972,739 + RPSI07_RS07445 0.32 +2.1 1,972,740 - RPSI07_RS07445 0.32 -0.5 1,972,740 - RPSI07_RS07445 0.32 -0.3 1,972,740 - RPSI07_RS07445 0.32 +0.9 1,972,880 + RPSI07_RS07445 0.47 +1.2 1,972,880 + RPSI07_RS07445 0.47 -0.7 1,972,880 + RPSI07_RS07445 0.47 +1.7 1,972,880 + RPSI07_RS07445 0.47 -1.7 1,972,880 + RPSI07_RS07445 0.47 -1.9 1,972,880 + RPSI07_RS07445 0.47 +0.1 1,972,880 + RPSI07_RS07445 0.47 +0.1 1,972,881 - RPSI07_RS07445 0.48 +0.7 1,972,881 - RPSI07_RS07445 0.48 -1.7 1,972,881 - RPSI07_RS07445 0.48 -0.9 1,972,881 - RPSI07_RS07445 0.48 -0.5 1,972,881 - RPSI07_RS07445 0.48 +0.6 1,972,895 + RPSI07_RS07445 0.49 -1.1 1,972,895 + RPSI07_RS07445 0.49 +0.3 1,972,895 + RPSI07_RS07445 0.49 -0.5 1,972,895 + RPSI07_RS07445 0.49 +0.0 1,972,896 - RPSI07_RS07445 0.49 -0.6 1,972,896 - RPSI07_RS07445 0.49 +0.3 1,972,896 - RPSI07_RS07445 0.49 +1.9 1,972,979 + RPSI07_RS07445 0.59 +2.7 1,972,980 - RPSI07_RS07445 0.59 +2.2 1,973,072 + RPSI07_RS07445 0.69 -1.1 1,973,072 + RPSI07_RS07445 0.69 -1.1 1,973,072 + RPSI07_RS07445 0.69 -0.6 1,973,094 - RPSI07_RS07445 0.71 -0.8 1,973,124 - RPSI07_RS07445 0.75 -0.9 1,973,264 + -0.6 1,973,264 + -0.0 1,973,264 + +1.1 1,973,265 - -0.1 1,973,267 + -1.9 1,973,267 + +1.4 1,973,268 - +0.2 1,973,268 - -1.5 1,973,268 - -0.8 1,973,421 + -1.7 1,973,421 + -0.5 1,973,421 + -1.0 1,973,421 + +0.4 1,973,421 + -0.5 1,973,422 - -0.1 1,973,422 - -2.4 1,973,482 + +1.5 1,973,508 + +2.6 1,973,508 + -0.7 1,973,508 + -2.6 1,973,508 + +0.1 1,973,508 + -1.0 1,973,509 - -0.2 1,973,509 - +0.1 1,973,509 - +0.8 1,973,536 - RPSI07_RS07450 0.10 +0.1 1,973,550 + RPSI07_RS07450 0.12 +0.3 1,973,550 + RPSI07_RS07450 0.12 +0.2 1,973,550 + RPSI07_RS07450 0.12 -0.7 1,973,551 - RPSI07_RS07450 0.12 -0.6 1,973,551 - RPSI07_RS07450 0.12 -0.5 1,973,577 + RPSI07_RS07450 0.14 -0.8 1,973,577 + RPSI07_RS07450 0.14 +1.1 1,973,577 + RPSI07_RS07450 0.14 -0.6 1,973,578 - RPSI07_RS07450 0.14 +0.5 1,973,578 - RPSI07_RS07450 0.14 -1.2 1,973,578 - RPSI07_RS07450 0.14 +3.3 1,973,676 + RPSI07_RS07450 0.23 +0.1 1,973,676 + RPSI07_RS07450 0.23 +2.1 1,973,677 - RPSI07_RS07450 0.23 -0.7 1,973,694 + RPSI07_RS07450 0.24 -0.2 1,973,694 + RPSI07_RS07450 0.24 -0.3 1,973,694 + RPSI07_RS07450 0.24 -0.2 1,973,695 - RPSI07_RS07450 0.24 +0.8 1,973,695 - RPSI07_RS07450 0.24 +1.1 1,973,892 + RPSI07_RS07450 0.42 +0.9 1,973,892 + RPSI07_RS07450 0.42 -1.2 1,973,925 + RPSI07_RS07450 0.45 +2.1 1,973,926 - RPSI07_RS07450 0.45 +0.1 1,973,926 - RPSI07_RS07450 0.45 +0.5 1,973,926 - RPSI07_RS07450 0.45 +1.1 1,974,034 - RPSI07_RS07450 0.54 +0.6 1,974,072 + RPSI07_RS07450 0.58 -0.6 1,974,072 + RPSI07_RS07450 0.58 -1.9 1,974,073 - RPSI07_RS07450 0.58 -1.7 1,974,073 - RPSI07_RS07450 0.58 +0.1 1,974,108 + RPSI07_RS07450 0.61 -0.3 1,974,108 + RPSI07_RS07450 0.61 +1.8 1,974,108 + RPSI07_RS07450 0.61 +0.7 1,974,108 + RPSI07_RS07450 0.61 -0.5 1,974,108 + RPSI07_RS07450 0.61 -0.4 1,974,108 + RPSI07_RS07450 0.61 -3.0 1,974,108 + RPSI07_RS07450 0.61 -0.1 1,974,108 + RPSI07_RS07450 0.61 +1.0 1,974,108 + RPSI07_RS07450 0.61 -0.7 1,974,109 - RPSI07_RS07450 0.61 -1.9 1,974,109 - RPSI07_RS07450 0.61 +1.1 1,974,109 - RPSI07_RS07450 0.61 +0.6 1,974,109 - RPSI07_RS07450 0.61 +0.5 1,974,109 - RPSI07_RS07450 0.61 -0.7 1,974,109 - RPSI07_RS07450 0.61 -0.4 1,974,109 - RPSI07_RS07450 0.61 +3.7 1,974,109 - RPSI07_RS07450 0.61 -0.3 1,974,109 - RPSI07_RS07450 0.61 -2.0 1,974,109 - RPSI07_RS07450 0.61 -1.0 1,974,109 - RPSI07_RS07450 0.61 -0.4 1,974,109 - RPSI07_RS07450 0.61 +0.0 1,974,109 - RPSI07_RS07450 0.61 +0.5 1,974,109 - RPSI07_RS07450 0.61 +0.2 1,974,109 - RPSI07_RS07450 0.61 +0.1 1,974,109 - RPSI07_RS07450 0.61 -0.8 1,974,109 - RPSI07_RS07450 0.61 +2.8 1,974,112 + RPSI07_RS07450 0.61 -1.6 1,974,113 - RPSI07_RS07450 0.61 -0.1 1,974,133 - RPSI07_RS07450 0.63 +1.0 1,974,186 + RPSI07_RS07450 0.68 -0.9 1,974,187 - RPSI07_RS07450 0.68 -0.1
Or see this region's nucleotide sequence