Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS24635 and RPSI07_RS24640 overlap by 51 nucleotides RPSI07_RS24640 and RPSI07_RS06575 are separated by 409 nucleotides RPSI07_RS06575 and RPSI07_RS06580 are separated by 288 nucleotides
RPSI07_RS24635: RPSI07_RS24635 - hypothetical protein, at 1,766,696 to 1,767,103
_RS24635
RPSI07_RS24640: RPSI07_RS24640 - hypothetical protein, at 1,767,053 to 1,767,445
_RS24640
RPSI07_RS06575: RPSI07_RS06575 - dihydrofolate reductase, at 1,767,855 to 1,768,517
_RS06575
RPSI07_RS06580: RPSI07_RS06580 - NAD(P)-dependent oxidoreductase, at 1,768,806 to 1,769,552
_RS06580
Position (kb)
1767
1768
1769 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1766.926 kb on + strand, within RPSI07_RS24635 at 1766.927 kb on - strand, within RPSI07_RS24635 at 1766.927 kb on - strand, within RPSI07_RS24635 at 1767.013 kb on + strand, within RPSI07_RS24635 at 1767.013 kb on + strand, within RPSI07_RS24635 at 1767.013 kb on + strand, within RPSI07_RS24635 at 1767.054 kb on + strand at 1767.054 kb on + strand at 1767.054 kb on + strand at 1767.055 kb on - strand at 1767.055 kb on - strand at 1767.055 kb on - strand at 1767.089 kb on + strand at 1767.091 kb on - strand at 1767.106 kb on + strand, within RPSI07_RS24640 at 1767.107 kb on - strand, within RPSI07_RS24640 at 1767.107 kb on - strand, within RPSI07_RS24640 at 1767.140 kb on - strand, within RPSI07_RS24640 at 1767.140 kb on - strand, within RPSI07_RS24640 at 1767.148 kb on - strand, within RPSI07_RS24640 at 1767.148 kb on - strand, within RPSI07_RS24640 at 1767.148 kb on - strand, within RPSI07_RS24640 at 1767.204 kb on - strand, within RPSI07_RS24640 at 1767.210 kb on + strand, within RPSI07_RS24640 at 1767.210 kb on + strand, within RPSI07_RS24640 at 1767.211 kb on - strand, within RPSI07_RS24640 at 1767.211 kb on - strand, within RPSI07_RS24640 at 1767.211 kb on - strand, within RPSI07_RS24640 at 1767.283 kb on + strand, within RPSI07_RS24640 at 1767.283 kb on + strand, within RPSI07_RS24640 at 1767.283 kb on + strand, within RPSI07_RS24640 at 1767.284 kb on - strand, within RPSI07_RS24640 at 1767.284 kb on - strand, within RPSI07_RS24640 at 1767.376 kb on - strand, within RPSI07_RS24640 at 1767.376 kb on - strand, within RPSI07_RS24640 at 1767.377 kb on + strand, within RPSI07_RS24640 at 1767.377 kb on + strand, within RPSI07_RS24640 at 1767.378 kb on - strand, within RPSI07_RS24640 at 1767.378 kb on - strand, within RPSI07_RS24640 at 1767.378 kb on - strand, within RPSI07_RS24640 at 1767.378 kb on - strand, within RPSI07_RS24640 at 1767.436 kb on + strand at 1767.437 kb on - strand at 1767.450 kb on + strand at 1767.451 kb on - strand at 1767.490 kb on - strand at 1767.494 kb on + strand at 1767.494 kb on + strand at 1767.494 kb on + strand at 1767.495 kb on - strand at 1767.495 kb on - strand at 1767.495 kb on - strand at 1767.497 kb on + strand at 1767.497 kb on + strand at 1767.498 kb on - strand at 1767.498 kb on - strand at 1767.498 kb on - strand at 1767.687 kb on - strand at 1767.708 kb on + strand at 1767.708 kb on + strand at 1767.718 kb on + strand at 1767.719 kb on + strand at 1767.719 kb on + strand at 1767.720 kb on - strand at 1767.720 kb on - strand at 1767.720 kb on - strand at 1767.720 kb on - strand at 1767.722 kb on + strand at 1767.723 kb on - strand at 1767.765 kb on + strand at 1767.765 kb on + strand at 1767.790 kb on + strand at 1767.791 kb on - strand at 1767.791 kb on - strand at 1767.793 kb on + strand at 1767.793 kb on + strand at 1767.793 kb on + strand at 1767.793 kb on + strand at 1767.793 kb on + strand at 1767.793 kb on + strand at 1767.794 kb on - strand at 1767.794 kb on - strand at 1767.794 kb on - strand at 1767.794 kb on - strand at 1767.794 kb on - strand at 1767.794 kb on - strand at 1767.824 kb on + strand at 1767.824 kb on + strand at 1767.825 kb on - strand at 1767.825 kb on - strand at 1767.825 kb on - strand at 1767.831 kb on + strand at 1767.832 kb on - strand at 1767.832 kb on - strand at 1767.840 kb on - strand at 1767.971 kb on - strand, within RPSI07_RS06575 at 1767.971 kb on - strand, within RPSI07_RS06575 at 1767.971 kb on - strand, within RPSI07_RS06575 at 1768.020 kb on - strand, within RPSI07_RS06575 at 1768.161 kb on + strand, within RPSI07_RS06575 at 1768.161 kb on + strand, within RPSI07_RS06575 at 1768.161 kb on + strand, within RPSI07_RS06575 at 1768.161 kb on + strand, within RPSI07_RS06575 at 1768.162 kb on - strand, within RPSI07_RS06575 at 1768.162 kb on - strand, within RPSI07_RS06575 at 1768.241 kb on - strand, within RPSI07_RS06575 at 1768.241 kb on - strand, within RPSI07_RS06575 at 1768.241 kb on - strand, within RPSI07_RS06575 at 1768.241 kb on - strand, within RPSI07_RS06575 at 1768.241 kb on - strand, within RPSI07_RS06575 at 1768.241 kb on - strand, within RPSI07_RS06575 at 1768.335 kb on + strand, within RPSI07_RS06575 at 1768.335 kb on + strand, within RPSI07_RS06575 at 1768.335 kb on + strand, within RPSI07_RS06575 at 1768.335 kb on + strand, within RPSI07_RS06575 at 1768.335 kb on + strand, within RPSI07_RS06575 at 1768.335 kb on + strand, within RPSI07_RS06575 at 1768.335 kb on + strand, within RPSI07_RS06575 at 1768.336 kb on - strand, within RPSI07_RS06575 at 1768.336 kb on - strand, within RPSI07_RS06575 at 1768.336 kb on - strand, within RPSI07_RS06575 at 1768.336 kb on - strand, within RPSI07_RS06575 at 1768.336 kb on - strand, within RPSI07_RS06575 at 1768.374 kb on - strand, within RPSI07_RS06575 at 1768.374 kb on - strand, within RPSI07_RS06575 at 1768.489 kb on + strand at 1768.490 kb on - strand at 1768.490 kb on - strand at 1768.515 kb on + strand at 1768.636 kb on + strand at 1768.695 kb on + strand at 1768.695 kb on + strand at 1768.695 kb on + strand at 1768.695 kb on + strand at 1768.695 kb on + strand at 1768.696 kb on - strand at 1768.696 kb on - strand at 1768.696 kb on - strand at 1768.696 kb on - strand at 1768.748 kb on + strand at 1768.748 kb on + strand at 1768.749 kb on - strand at 1768.749 kb on - strand at 1768.923 kb on + strand, within RPSI07_RS06580 at 1768.924 kb on + strand, within RPSI07_RS06580 at 1768.924 kb on + strand, within RPSI07_RS06580 at 1768.925 kb on - strand, within RPSI07_RS06580 at 1768.925 kb on - strand, within RPSI07_RS06580 at 1768.925 kb on - strand, within RPSI07_RS06580 at 1768.925 kb on - strand, within RPSI07_RS06580 at 1768.983 kb on - strand, within RPSI07_RS06580 at 1769.023 kb on + strand, within RPSI07_RS06580 at 1769.023 kb on + strand, within RPSI07_RS06580 at 1769.086 kb on + strand, within RPSI07_RS06580 at 1769.086 kb on + strand, within RPSI07_RS06580 at 1769.087 kb on - strand, within RPSI07_RS06580 at 1769.087 kb on - strand, within RPSI07_RS06580 at 1769.191 kb on + strand, within RPSI07_RS06580 at 1769.191 kb on + strand, within RPSI07_RS06580 at 1769.192 kb on - strand, within RPSI07_RS06580 at 1769.192 kb on - strand, within RPSI07_RS06580 at 1769.207 kb on + strand, within RPSI07_RS06580 at 1769.207 kb on + strand, within RPSI07_RS06580 at 1769.207 kb on + strand, within RPSI07_RS06580 at 1769.208 kb on - strand, within RPSI07_RS06580 at 1769.208 kb on - strand, within RPSI07_RS06580 at 1769.350 kb on + strand, within RPSI07_RS06580 at 1769.350 kb on + strand, within RPSI07_RS06580 at 1769.448 kb on + strand, within RPSI07_RS06580 at 1769.504 kb on + strand at 1769.504 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,766,926 + RPSI07_RS24635 0.56 -0.3 1,766,927 - RPSI07_RS24635 0.57 -1.2 1,766,927 - RPSI07_RS24635 0.57 -1.9 1,767,013 + RPSI07_RS24635 0.78 +0.5 1,767,013 + RPSI07_RS24635 0.78 -0.6 1,767,013 + RPSI07_RS24635 0.78 -0.3 1,767,054 + -0.0 1,767,054 + +1.1 1,767,054 + +0.3 1,767,055 - +0.1 1,767,055 - +0.0 1,767,055 - -1.5 1,767,089 + +1.1 1,767,091 - -0.7 1,767,106 + RPSI07_RS24640 0.13 -0.8 1,767,107 - RPSI07_RS24640 0.14 -0.1 1,767,107 - RPSI07_RS24640 0.14 -0.9 1,767,140 - RPSI07_RS24640 0.22 +1.4 1,767,140 - RPSI07_RS24640 0.22 +0.5 1,767,148 - RPSI07_RS24640 0.24 -1.1 1,767,148 - RPSI07_RS24640 0.24 -1.1 1,767,148 - RPSI07_RS24640 0.24 -1.2 1,767,204 - RPSI07_RS24640 0.38 -1.5 1,767,210 + RPSI07_RS24640 0.40 -0.3 1,767,210 + RPSI07_RS24640 0.40 -0.4 1,767,211 - RPSI07_RS24640 0.40 +0.3 1,767,211 - RPSI07_RS24640 0.40 -0.9 1,767,211 - RPSI07_RS24640 0.40 -1.4 1,767,283 + RPSI07_RS24640 0.59 +0.2 1,767,283 + RPSI07_RS24640 0.59 -1.0 1,767,283 + RPSI07_RS24640 0.59 -1.8 1,767,284 - RPSI07_RS24640 0.59 +0.1 1,767,284 - RPSI07_RS24640 0.59 +0.6 1,767,376 - RPSI07_RS24640 0.82 -1.0 1,767,376 - RPSI07_RS24640 0.82 -1.0 1,767,377 + RPSI07_RS24640 0.82 -1.4 1,767,377 + RPSI07_RS24640 0.82 -0.0 1,767,378 - RPSI07_RS24640 0.83 -0.2 1,767,378 - RPSI07_RS24640 0.83 +0.0 1,767,378 - RPSI07_RS24640 0.83 +0.4 1,767,378 - RPSI07_RS24640 0.83 -0.8 1,767,436 + -0.2 1,767,437 - +0.9 1,767,450 + -1.2 1,767,451 - -0.0 1,767,490 - +1.5 1,767,494 + +0.5 1,767,494 + -1.3 1,767,494 + +0.2 1,767,495 - +0.2 1,767,495 - +3.4 1,767,495 - -0.2 1,767,497 + +0.3 1,767,497 + +1.1 1,767,498 - +0.2 1,767,498 - +0.9 1,767,498 - -1.1 1,767,687 - +0.1 1,767,708 + -0.7 1,767,708 + -0.1 1,767,718 + -0.5 1,767,719 + -1.2 1,767,719 + +0.8 1,767,720 - -0.0 1,767,720 - +0.5 1,767,720 - +1.0 1,767,720 - +0.6 1,767,722 + -0.8 1,767,723 - -2.2 1,767,765 + -2.8 1,767,765 + -0.5 1,767,790 + +0.5 1,767,791 - +0.3 1,767,791 - +0.4 1,767,793 + +1.1 1,767,793 + -2.7 1,767,793 + -0.7 1,767,793 + -0.2 1,767,793 + -1.7 1,767,793 + -0.4 1,767,794 - +0.5 1,767,794 - +0.5 1,767,794 - -0.9 1,767,794 - -0.9 1,767,794 - +0.4 1,767,794 - -0.4 1,767,824 + +0.6 1,767,824 + -2.1 1,767,825 - -0.1 1,767,825 - +0.1 1,767,825 - -0.3 1,767,831 + +0.1 1,767,832 - -1.0 1,767,832 - -0.5 1,767,840 - +3.7 1,767,971 - RPSI07_RS06575 0.17 -1.7 1,767,971 - RPSI07_RS06575 0.17 +1.1 1,767,971 - RPSI07_RS06575 0.17 -1.2 1,768,020 - RPSI07_RS06575 0.25 +1.1 1,768,161 + RPSI07_RS06575 0.46 -1.4 1,768,161 + RPSI07_RS06575 0.46 -0.3 1,768,161 + RPSI07_RS06575 0.46 +0.1 1,768,161 + RPSI07_RS06575 0.46 -0.2 1,768,162 - RPSI07_RS06575 0.46 -0.3 1,768,162 - RPSI07_RS06575 0.46 +0.2 1,768,241 - RPSI07_RS06575 0.58 +1.4 1,768,241 - RPSI07_RS06575 0.58 -1.0 1,768,241 - RPSI07_RS06575 0.58 -1.0 1,768,241 - RPSI07_RS06575 0.58 -0.1 1,768,241 - RPSI07_RS06575 0.58 +3.3 1,768,241 - RPSI07_RS06575 0.58 +0.1 1,768,335 + RPSI07_RS06575 0.72 -0.5 1,768,335 + RPSI07_RS06575 0.72 +0.2 1,768,335 + RPSI07_RS06575 0.72 -0.9 1,768,335 + RPSI07_RS06575 0.72 +0.1 1,768,335 + RPSI07_RS06575 0.72 +0.6 1,768,335 + RPSI07_RS06575 0.72 +0.5 1,768,335 + RPSI07_RS06575 0.72 +0.4 1,768,336 - RPSI07_RS06575 0.73 -0.6 1,768,336 - RPSI07_RS06575 0.73 -0.5 1,768,336 - RPSI07_RS06575 0.73 -1.6 1,768,336 - RPSI07_RS06575 0.73 -3.3 1,768,336 - RPSI07_RS06575 0.73 -0.9 1,768,374 - RPSI07_RS06575 0.78 +1.3 1,768,374 - RPSI07_RS06575 0.78 +0.3 1,768,489 + -0.1 1,768,490 - +1.1 1,768,490 - -0.4 1,768,515 + +0.2 1,768,636 + -1.2 1,768,695 + +4.5 1,768,695 + -1.3 1,768,695 + +0.0 1,768,695 + -0.0 1,768,695 + +0.4 1,768,696 - +1.1 1,768,696 - -1.2 1,768,696 - -0.9 1,768,696 - +1.4 1,768,748 + -1.2 1,768,748 + +0.5 1,768,749 - +1.3 1,768,749 - -1.2 1,768,923 + RPSI07_RS06580 0.16 +1.7 1,768,924 + RPSI07_RS06580 0.16 -0.6 1,768,924 + RPSI07_RS06580 0.16 -0.1 1,768,925 - RPSI07_RS06580 0.16 -0.4 1,768,925 - RPSI07_RS06580 0.16 -1.8 1,768,925 - RPSI07_RS06580 0.16 -0.2 1,768,925 - RPSI07_RS06580 0.16 -4.0 1,768,983 - RPSI07_RS06580 0.24 -1.0 1,769,023 + RPSI07_RS06580 0.29 +2.5 1,769,023 + RPSI07_RS06580 0.29 -0.9 1,769,086 + RPSI07_RS06580 0.37 +2.1 1,769,086 + RPSI07_RS06580 0.37 +4.3 1,769,087 - RPSI07_RS06580 0.38 -0.1 1,769,087 - RPSI07_RS06580 0.38 -0.0 1,769,191 + RPSI07_RS06580 0.52 -0.1 1,769,191 + RPSI07_RS06580 0.52 +0.1 1,769,192 - RPSI07_RS06580 0.52 -0.7 1,769,192 - RPSI07_RS06580 0.52 -0.6 1,769,207 + RPSI07_RS06580 0.54 -0.4 1,769,207 + RPSI07_RS06580 0.54 +0.2 1,769,207 + RPSI07_RS06580 0.54 -0.8 1,769,208 - RPSI07_RS06580 0.54 -0.3 1,769,208 - RPSI07_RS06580 0.54 -1.7 1,769,350 + RPSI07_RS06580 0.73 +0.9 1,769,350 + RPSI07_RS06580 0.73 -0.1 1,769,448 + RPSI07_RS06580 0.86 -0.0 1,769,504 + +1.5 1,769,504 + -0.9
Or see this region's nucleotide sequence