Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS03845 and RPSI07_RS03850 are separated by 240 nucleotides RPSI07_RS03850 and RPSI07_RS03855 are separated by 419 nucleotides RPSI07_RS03855 and RPSI07_RS03860 are separated by 71 nucleotides
RPSI07_RS03845: RPSI07_RS03845 - aminotransferase class V-fold PLP-dependent enzyme, at 1,045,588 to 1,046,745
_RS03845
RPSI07_RS03850: RPSI07_RS03850 - hypothetical protein, at 1,046,986 to 1,047,231
_RS03850
RPSI07_RS03855: RPSI07_RS03855 - hypothetical protein, at 1,047,651 to 1,048,058
_RS03855
RPSI07_RS03860: RPSI07_RS03860 - membrane protein, at 1,048,130 to 1,049,434
_RS03860
Position (kb)
1047
1048
1049 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1046.683 kb on + strand at 1046.683 kb on + strand at 1046.684 kb on - strand at 1046.684 kb on - strand at 1046.684 kb on - strand at 1046.684 kb on - strand at 1046.735 kb on + strand at 1046.735 kb on + strand at 1046.736 kb on - strand at 1046.767 kb on + strand at 1046.767 kb on + strand at 1046.767 kb on + strand at 1046.768 kb on - strand at 1046.768 kb on - strand at 1046.768 kb on - strand at 1046.768 kb on - strand at 1046.807 kb on - strand at 1046.807 kb on - strand at 1046.895 kb on + strand at 1046.896 kb on - strand at 1047.002 kb on + strand at 1047.002 kb on + strand at 1047.002 kb on + strand at 1047.229 kb on + strand at 1047.229 kb on + strand at 1047.230 kb on - strand at 1047.230 kb on - strand at 1047.506 kb on + strand at 1047.507 kb on - strand at 1047.507 kb on - strand at 1047.621 kb on + strand at 1047.622 kb on - strand at 1047.622 kb on - strand at 1047.684 kb on - strand at 1047.732 kb on + strand, within RPSI07_RS03855 at 1047.733 kb on - strand, within RPSI07_RS03855 at 1047.733 kb on - strand, within RPSI07_RS03855 at 1048.031 kb on + strand at 1048.032 kb on - strand at 1048.056 kb on + strand at 1048.128 kb on + strand at 1048.129 kb on + strand at 1048.129 kb on + strand at 1048.129 kb on + strand at 1048.129 kb on + strand at 1048.129 kb on + strand at 1048.129 kb on + strand at 1048.129 kb on + strand at 1048.129 kb on + strand at 1048.129 kb on + strand at 1048.129 kb on + strand at 1048.130 kb on - strand at 1048.130 kb on - strand at 1048.130 kb on - strand at 1048.130 kb on - strand at 1048.130 kb on - strand at 1048.130 kb on - strand at 1048.132 kb on - strand at 1048.370 kb on - strand, within RPSI07_RS03860 at 1048.552 kb on + strand, within RPSI07_RS03860 at 1048.552 kb on + strand, within RPSI07_RS03860 at 1048.552 kb on + strand, within RPSI07_RS03860 at 1048.552 kb on + strand, within RPSI07_RS03860 at 1048.552 kb on + strand, within RPSI07_RS03860 at 1048.552 kb on + strand, within RPSI07_RS03860 at 1048.553 kb on - strand, within RPSI07_RS03860 at 1048.584 kb on - strand, within RPSI07_RS03860 at 1048.984 kb on + strand, within RPSI07_RS03860 at 1048.985 kb on - strand, within RPSI07_RS03860
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,046,683 + +0.7 1,046,683 + -1.2 1,046,684 - +0.4 1,046,684 - -0.9 1,046,684 - -0.0 1,046,684 - -1.6 1,046,735 + +0.8 1,046,735 + -0.2 1,046,736 - -1.0 1,046,767 + -1.0 1,046,767 + +0.7 1,046,767 + +0.3 1,046,768 - +0.3 1,046,768 - -0.2 1,046,768 - -0.6 1,046,768 - -0.3 1,046,807 - +0.4 1,046,807 - +0.5 1,046,895 + +0.2 1,046,896 - +0.0 1,047,002 + -0.1 1,047,002 + +0.5 1,047,002 + -2.4 1,047,229 + -1.6 1,047,229 + +0.1 1,047,230 - -0.4 1,047,230 - -1.3 1,047,506 + -1.0 1,047,507 - -0.1 1,047,507 - +0.4 1,047,621 + +1.5 1,047,622 - -0.0 1,047,622 - -0.4 1,047,684 - +2.6 1,047,732 + RPSI07_RS03855 0.20 +0.2 1,047,733 - RPSI07_RS03855 0.20 +0.1 1,047,733 - RPSI07_RS03855 0.20 +0.5 1,048,031 + -1.0 1,048,032 - -0.5 1,048,056 + -0.6 1,048,128 + +0.2 1,048,129 + -1.6 1,048,129 + -0.7 1,048,129 + +0.9 1,048,129 + -1.3 1,048,129 + +0.1 1,048,129 + +0.6 1,048,129 + +2.2 1,048,129 + +0.6 1,048,129 + -1.8 1,048,129 + -0.1 1,048,130 - -0.4 1,048,130 - +0.6 1,048,130 - +0.6 1,048,130 - +0.6 1,048,130 - -1.4 1,048,130 - +0.6 1,048,132 - -0.8 1,048,370 - RPSI07_RS03860 0.18 +3.6 1,048,552 + RPSI07_RS03860 0.32 +1.6 1,048,552 + RPSI07_RS03860 0.32 -0.4 1,048,552 + RPSI07_RS03860 0.32 -1.3 1,048,552 + RPSI07_RS03860 0.32 -0.6 1,048,552 + RPSI07_RS03860 0.32 -0.2 1,048,552 + RPSI07_RS03860 0.32 -0.4 1,048,553 - RPSI07_RS03860 0.32 -0.8 1,048,584 - RPSI07_RS03860 0.35 -0.8 1,048,984 + RPSI07_RS03860 0.65 -0.2 1,048,985 - RPSI07_RS03860 0.66 -1.0
Or see this region's nucleotide sequence