Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS02050 and RPSI07_RS02055 are separated by 574 nucleotides RPSI07_RS02055 and RPSI07_RS24030 are separated by 527 nucleotides
RPSI07_RS02050: RPSI07_RS02050 - peptidase M1, at 602,980 to 605,190
_RS02050
RPSI07_RS02055: RPSI07_RS02055 - hypothetical protein, at 605,765 to 606,205
_RS02055
RPSI07_RS24030: RPSI07_RS24030 - hypothetical protein, at 606,733 to 607,430
_RS24030
Position (kb) 605
606
607 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 604.840 kb on + strand, within RPSI07_RS02050 at 604.840 kb on + strand, within RPSI07_RS02050 at 604.841 kb on - strand, within RPSI07_RS02050 at 604.841 kb on - strand, within RPSI07_RS02050 at 604.898 kb on + strand, within RPSI07_RS02050 at 604.898 kb on + strand, within RPSI07_RS02050 at 604.898 kb on + strand, within RPSI07_RS02050 at 604.899 kb on - strand, within RPSI07_RS02050 at 604.899 kb on - strand, within RPSI07_RS02050 at 604.899 kb on - strand, within RPSI07_RS02050 at 604.899 kb on - strand, within RPSI07_RS02050 at 604.899 kb on - strand, within RPSI07_RS02050 at 604.915 kb on - strand, within RPSI07_RS02050 at 604.915 kb on - strand, within RPSI07_RS02050 at 604.930 kb on + strand, within RPSI07_RS02050 at 605.028 kb on + strand at 605.028 kb on + strand at 605.028 kb on + strand at 605.028 kb on + strand at 605.029 kb on - strand at 605.029 kb on - strand at 605.029 kb on - strand at 605.163 kb on + strand at 605.163 kb on + strand at 605.533 kb on - strand at 605.688 kb on + strand at 605.689 kb on + strand at 605.701 kb on + strand at 605.796 kb on + strand at 606.048 kb on + strand, within RPSI07_RS02055 at 606.048 kb on + strand, within RPSI07_RS02055 at 606.048 kb on + strand, within RPSI07_RS02055 at 606.048 kb on + strand, within RPSI07_RS02055 at 606.048 kb on + strand, within RPSI07_RS02055 at 606.048 kb on + strand, within RPSI07_RS02055 at 606.048 kb on + strand, within RPSI07_RS02055 at 606.048 kb on + strand, within RPSI07_RS02055 at 606.049 kb on - strand, within RPSI07_RS02055 at 606.049 kb on - strand, within RPSI07_RS02055 at 606.049 kb on - strand, within RPSI07_RS02055 at 606.049 kb on - strand, within RPSI07_RS02055 at 606.049 kb on - strand, within RPSI07_RS02055 at 606.049 kb on - strand, within RPSI07_RS02055 at 606.220 kb on + strand at 606.416 kb on + strand at 606.416 kb on + strand at 606.416 kb on + strand at 606.416 kb on + strand at 606.416 kb on + strand at 606.416 kb on + strand at 606.416 kb on + strand at 606.417 kb on - strand at 606.417 kb on - strand at 606.448 kb on + strand at 606.583 kb on + strand at 606.583 kb on + strand at 606.753 kb on + strand at 606.754 kb on - strand at 606.754 kb on - strand at 606.815 kb on + strand, within RPSI07_RS24030 at 606.845 kb on + strand, within RPSI07_RS24030 at 606.846 kb on - strand, within RPSI07_RS24030 at 606.846 kb on - strand, within RPSI07_RS24030 at 606.846 kb on - strand, within RPSI07_RS24030 at 606.846 kb on - strand, within RPSI07_RS24030 at 606.846 kb on - strand, within RPSI07_RS24030 at 606.846 kb on - strand, within RPSI07_RS24030 at 606.887 kb on - strand, within RPSI07_RS24030 at 606.887 kb on - strand, within RPSI07_RS24030 at 606.887 kb on - strand, within RPSI07_RS24030 at 607.010 kb on - strand, within RPSI07_RS24030 at 607.109 kb on + strand, within RPSI07_RS24030 at 607.109 kb on + strand, within RPSI07_RS24030 at 607.135 kb on + strand, within RPSI07_RS24030 at 607.135 kb on + strand, within RPSI07_RS24030 at 607.136 kb on - strand, within RPSI07_RS24030 at 607.136 kb on - strand, within RPSI07_RS24030 at 607.136 kb on - strand, within RPSI07_RS24030 at 607.145 kb on + strand, within RPSI07_RS24030 at 607.145 kb on + strand, within RPSI07_RS24030 at 607.145 kb on + strand, within RPSI07_RS24030 at 607.145 kb on + strand, within RPSI07_RS24030 at 607.146 kb on - strand, within RPSI07_RS24030
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 604,840 + RPSI07_RS02050 0.84 -2.1 604,840 + RPSI07_RS02050 0.84 +0.0 604,841 - RPSI07_RS02050 0.84 -0.9 604,841 - RPSI07_RS02050 0.84 -0.4 604,898 + RPSI07_RS02050 0.87 -1.8 604,898 + RPSI07_RS02050 0.87 -0.2 604,898 + RPSI07_RS02050 0.87 -0.7 604,899 - RPSI07_RS02050 0.87 -0.1 604,899 - RPSI07_RS02050 0.87 -0.2 604,899 - RPSI07_RS02050 0.87 +1.1 604,899 - RPSI07_RS02050 0.87 -0.2 604,899 - RPSI07_RS02050 0.87 +0.6 604,915 - RPSI07_RS02050 0.88 -0.3 604,915 - RPSI07_RS02050 0.88 +0.3 604,930 + RPSI07_RS02050 0.88 -0.1 605,028 + -0.4 605,028 + -0.6 605,028 + +0.2 605,028 + -0.5 605,029 - -0.4 605,029 - +0.4 605,029 - -1.3 605,163 + -0.2 605,163 + +0.6 605,533 - +0.9 605,688 + -0.5 605,689 + +0.1 605,701 + +0.4 605,796 + -0.4 606,048 + RPSI07_RS02055 0.64 -1.1 606,048 + RPSI07_RS02055 0.64 +0.4 606,048 + RPSI07_RS02055 0.64 +0.5 606,048 + RPSI07_RS02055 0.64 -0.1 606,048 + RPSI07_RS02055 0.64 +0.1 606,048 + RPSI07_RS02055 0.64 -0.7 606,048 + RPSI07_RS02055 0.64 -0.4 606,048 + RPSI07_RS02055 0.64 +0.1 606,049 - RPSI07_RS02055 0.64 +2.8 606,049 - RPSI07_RS02055 0.64 -0.1 606,049 - RPSI07_RS02055 0.64 -0.3 606,049 - RPSI07_RS02055 0.64 -1.3 606,049 - RPSI07_RS02055 0.64 -0.4 606,049 - RPSI07_RS02055 0.64 -0.4 606,220 + -0.1 606,416 + -0.3 606,416 + +0.5 606,416 + +0.3 606,416 + +0.3 606,416 + -0.0 606,416 + -0.8 606,416 + +0.1 606,417 - +2.7 606,417 - -0.3 606,448 + -0.7 606,583 + -0.2 606,583 + -0.3 606,753 + +1.1 606,754 - -0.3 606,754 - -1.4 606,815 + RPSI07_RS24030 0.12 -0.5 606,845 + RPSI07_RS24030 0.16 -1.9 606,846 - RPSI07_RS24030 0.16 -0.5 606,846 - RPSI07_RS24030 0.16 +0.5 606,846 - RPSI07_RS24030 0.16 +0.5 606,846 - RPSI07_RS24030 0.16 -0.1 606,846 - RPSI07_RS24030 0.16 +1.0 606,846 - RPSI07_RS24030 0.16 -2.3 606,887 - RPSI07_RS24030 0.22 -0.1 606,887 - RPSI07_RS24030 0.22 -0.8 606,887 - RPSI07_RS24030 0.22 -1.4 607,010 - RPSI07_RS24030 0.40 -1.3 607,109 + RPSI07_RS24030 0.54 +1.1 607,109 + RPSI07_RS24030 0.54 +1.1 607,135 + RPSI07_RS24030 0.58 -3.0 607,135 + RPSI07_RS24030 0.58 -0.3 607,136 - RPSI07_RS24030 0.58 +2.1 607,136 - RPSI07_RS24030 0.58 -0.5 607,136 - RPSI07_RS24030 0.58 -1.6 607,145 + RPSI07_RS24030 0.59 -0.4 607,145 + RPSI07_RS24030 0.59 -0.9 607,145 + RPSI07_RS24030 0.59 -0.7 607,145 + RPSI07_RS24030 0.59 -0.1 607,146 - RPSI07_RS24030 0.59 +2.2
Or see this region's nucleotide sequence