Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS01540 and RPSI07_RS01545 are separated by 35 nucleotides RPSI07_RS01545 and RPSI07_RS01550 overlap by 11 nucleotides RPSI07_RS01550 and RPSI07_RS01555 are separated by 93 nucleotides RPSI07_RS01555 and RPSI07_RS01560 are separated by 20 nucleotides
RPSI07_RS01540: RPSI07_RS01540 - flagellar motor switch protein FliG, at 408,853 to 409,845
_RS01540
RPSI07_RS01545: RPSI07_RS01545 - flagellar assembly protein FliH, at 409,881 to 410,627
_RS01545
RPSI07_RS01550: RPSI07_RS01550 - flagellum-specific ATP synthase FliI, at 410,617 to 412,062
_RS01550
RPSI07_RS01555: RPSI07_RS01555 - flagellar export protein FliJ, at 412,156 to 412,620
_RS01555
RPSI07_RS01560: RPSI07_RS01560 - flagellar hook-length control protein FliK, at 412,641 to 414,176
_RS01560
Position (kb) 410
411
412
413 Strain fitness (log2 ratio) -2
-1
0
1
2 at 409.837 kb on + strand at 409.838 kb on - strand at 409.838 kb on - strand at 409.838 kb on - strand at 409.838 kb on - strand at 409.910 kb on + strand at 409.911 kb on - strand at 409.911 kb on - strand at 409.924 kb on - strand at 409.924 kb on - strand at 410.796 kb on - strand, within RPSI07_RS01550 at 410.796 kb on - strand, within RPSI07_RS01550 at 410.836 kb on + strand, within RPSI07_RS01550 at 410.970 kb on + strand, within RPSI07_RS01550 at 411.278 kb on - strand, within RPSI07_RS01550 at 411.440 kb on - strand, within RPSI07_RS01550 at 411.461 kb on - strand, within RPSI07_RS01550 at 411.461 kb on - strand, within RPSI07_RS01550 at 411.514 kb on + strand, within RPSI07_RS01550 at 411.514 kb on + strand, within RPSI07_RS01550 at 411.673 kb on + strand, within RPSI07_RS01550 at 411.673 kb on + strand, within RPSI07_RS01550 at 411.674 kb on - strand, within RPSI07_RS01550 at 411.674 kb on - strand, within RPSI07_RS01550 at 411.784 kb on + strand, within RPSI07_RS01550 at 411.880 kb on + strand, within RPSI07_RS01550 at 411.881 kb on - strand, within RPSI07_RS01550 at 411.925 kb on + strand at 411.970 kb on + strand at 412.098 kb on + strand at 412.099 kb on - strand at 412.295 kb on - strand, within RPSI07_RS01555 at 412.303 kb on - strand, within RPSI07_RS01555 at 412.303 kb on - strand, within RPSI07_RS01555 at 412.303 kb on - strand, within RPSI07_RS01555 at 412.303 kb on - strand, within RPSI07_RS01555 at 412.303 kb on - strand, within RPSI07_RS01555 at 412.303 kb on - strand, within RPSI07_RS01555 at 412.307 kb on - strand, within RPSI07_RS01555 at 412.307 kb on - strand, within RPSI07_RS01555 at 412.366 kb on + strand, within RPSI07_RS01555 at 412.366 kb on + strand, within RPSI07_RS01555 at 412.366 kb on + strand, within RPSI07_RS01555 at 412.366 kb on + strand, within RPSI07_RS01555 at 412.367 kb on - strand, within RPSI07_RS01555 at 412.367 kb on - strand, within RPSI07_RS01555 at 412.367 kb on - strand, within RPSI07_RS01555 at 412.367 kb on - strand, within RPSI07_RS01555 at 412.367 kb on - strand, within RPSI07_RS01555 at 412.749 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 409,837 + -1.5 409,838 - +0.5 409,838 - -1.7 409,838 - -0.3 409,838 - -0.4 409,910 + +0.9 409,911 - +1.9 409,911 - +0.9 409,924 - +1.1 409,924 - -1.0 410,796 - RPSI07_RS01550 0.12 -0.7 410,796 - RPSI07_RS01550 0.12 +0.3 410,836 + RPSI07_RS01550 0.15 +0.1 410,970 + RPSI07_RS01550 0.24 -1.3 411,278 - RPSI07_RS01550 0.46 -1.4 411,440 - RPSI07_RS01550 0.57 +0.3 411,461 - RPSI07_RS01550 0.58 +0.6 411,461 - RPSI07_RS01550 0.58 +0.0 411,514 + RPSI07_RS01550 0.62 +1.1 411,514 + RPSI07_RS01550 0.62 +0.0 411,673 + RPSI07_RS01550 0.73 +2.1 411,673 + RPSI07_RS01550 0.73 -0.4 411,674 - RPSI07_RS01550 0.73 -0.7 411,674 - RPSI07_RS01550 0.73 +1.1 411,784 + RPSI07_RS01550 0.81 -1.3 411,880 + RPSI07_RS01550 0.87 +0.5 411,881 - RPSI07_RS01550 0.87 -0.6 411,925 + -0.8 411,970 + -0.6 412,098 + -0.1 412,099 - -0.7 412,295 - RPSI07_RS01555 0.30 -0.8 412,303 - RPSI07_RS01555 0.32 -1.7 412,303 - RPSI07_RS01555 0.32 +0.7 412,303 - RPSI07_RS01555 0.32 -2.7 412,303 - RPSI07_RS01555 0.32 +0.2 412,303 - RPSI07_RS01555 0.32 -0.3 412,303 - RPSI07_RS01555 0.32 -2.1 412,307 - RPSI07_RS01555 0.32 +0.4 412,307 - RPSI07_RS01555 0.32 -1.3 412,366 + RPSI07_RS01555 0.45 -0.9 412,366 + RPSI07_RS01555 0.45 +0.5 412,366 + RPSI07_RS01555 0.45 -0.7 412,366 + RPSI07_RS01555 0.45 -1.4 412,367 - RPSI07_RS01555 0.45 +0.2 412,367 - RPSI07_RS01555 0.45 -1.7 412,367 - RPSI07_RS01555 0.45 +1.1 412,367 - RPSI07_RS01555 0.45 +0.5 412,367 - RPSI07_RS01555 0.45 -0.0 412,749 - -0.9
Or see this region's nucleotide sequence