Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11180 and RPSI07_RS11185 are separated by 141 nucleotides RPSI07_RS11185 and RPSI07_RS11190 are separated by 38 nucleotides
RPSI07_RS11180: RPSI07_RS11180 - EamA family transporter, at 709,796 to 710,695
_RS11180
RPSI07_RS11185: RPSI07_RS11185 - Lrp/AsnC family transcriptional regulator, at 710,837 to 711,313
_RS11185
RPSI07_RS11190: RPSI07_RS11190 - hypothetical protein, at 711,352 to 712,698
_RS11190
Position (kb) 710
711
712 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 710.182 kb on + strand, within RPSI07_RS11180 at 710.183 kb on - strand, within RPSI07_RS11180 at 710.183 kb on - strand, within RPSI07_RS11180 at 710.184 kb on + strand, within RPSI07_RS11180 at 710.184 kb on + strand, within RPSI07_RS11180 at 710.184 kb on + strand, within RPSI07_RS11180 at 710.185 kb on - strand, within RPSI07_RS11180 at 710.185 kb on - strand, within RPSI07_RS11180 at 710.185 kb on - strand, within RPSI07_RS11180 at 710.217 kb on + strand, within RPSI07_RS11180 at 710.406 kb on + strand, within RPSI07_RS11180 at 710.407 kb on - strand, within RPSI07_RS11180 at 710.442 kb on + strand, within RPSI07_RS11180 at 710.443 kb on - strand, within RPSI07_RS11180 at 710.728 kb on + strand at 710.729 kb on - strand at 710.858 kb on - strand at 710.866 kb on - strand at 711.128 kb on + strand, within RPSI07_RS11185 at 711.129 kb on - strand, within RPSI07_RS11185 at 711.129 kb on - strand, within RPSI07_RS11185 at 711.189 kb on - strand, within RPSI07_RS11185 at 711.189 kb on - strand, within RPSI07_RS11185 at 711.272 kb on + strand at 711.273 kb on - strand at 711.680 kb on + strand, within RPSI07_RS11190 at 711.680 kb on + strand, within RPSI07_RS11190 at 711.981 kb on - strand, within RPSI07_RS11190 at 711.996 kb on + strand, within RPSI07_RS11190 at 711.996 kb on + strand, within RPSI07_RS11190 at 711.997 kb on - strand, within RPSI07_RS11190 at 711.997 kb on - strand, within RPSI07_RS11190 at 712.010 kb on + strand, within RPSI07_RS11190 at 712.122 kb on - strand, within RPSI07_RS11190 at 712.122 kb on - strand, within RPSI07_RS11190 at 712.148 kb on + strand, within RPSI07_RS11190 at 712.148 kb on + strand, within RPSI07_RS11190 at 712.149 kb on - strand, within RPSI07_RS11190
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 710,182 + RPSI07_RS11180 0.43 +0.4 710,183 - RPSI07_RS11180 0.43 -0.1 710,183 - RPSI07_RS11180 0.43 -1.4 710,184 + RPSI07_RS11180 0.43 -0.8 710,184 + RPSI07_RS11180 0.43 +1.5 710,184 + RPSI07_RS11180 0.43 -1.7 710,185 - RPSI07_RS11180 0.43 -0.0 710,185 - RPSI07_RS11180 0.43 +0.6 710,185 - RPSI07_RS11180 0.43 +0.1 710,217 + RPSI07_RS11180 0.47 +0.7 710,406 + RPSI07_RS11180 0.68 -0.7 710,407 - RPSI07_RS11180 0.68 +2.3 710,442 + RPSI07_RS11180 0.72 -1.0 710,443 - RPSI07_RS11180 0.72 +1.3 710,728 + -0.4 710,729 - -0.6 710,858 - -2.5 710,866 - +0.2 711,128 + RPSI07_RS11185 0.61 -0.4 711,129 - RPSI07_RS11185 0.61 +0.8 711,129 - RPSI07_RS11185 0.61 -0.3 711,189 - RPSI07_RS11185 0.74 +1.1 711,189 - RPSI07_RS11185 0.74 +0.0 711,272 + +0.2 711,273 - +0.3 711,680 + RPSI07_RS11190 0.24 -0.4 711,680 + RPSI07_RS11190 0.24 +0.8 711,981 - RPSI07_RS11190 0.47 -0.4 711,996 + RPSI07_RS11190 0.48 +5.6 711,996 + RPSI07_RS11190 0.48 -1.0 711,997 - RPSI07_RS11190 0.48 +0.7 711,997 - RPSI07_RS11190 0.48 +1.0 712,010 + RPSI07_RS11190 0.49 -0.1 712,122 - RPSI07_RS11190 0.57 +0.9 712,122 - RPSI07_RS11190 0.57 -0.1 712,148 + RPSI07_RS11190 0.59 +0.3 712,148 + RPSI07_RS11190 0.59 -0.1 712,149 - RPSI07_RS11190 0.59 +3.1
Or see this region's nucleotide sequence