Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10635 and RPSI07_RS10640 are separated by 103 nucleotides RPSI07_RS10640 and RPSI07_RS10645 are separated by 190 nucleotides RPSI07_RS10645 and RPSI07_RS10650 are separated by 98 nucleotides
RPSI07_RS10635: RPSI07_RS10635 - peptide chain release factor N(5)-glutamine methyltransferase, at 590,458 to 591,345
_RS10635
RPSI07_RS10640: RPSI07_RS10640 - hypothetical protein, at 591,449 to 591,781
_RS10640
RPSI07_RS10645: RPSI07_RS10645 - peptide chain release factor 1, at 591,972 to 593,054
_RS10645
RPSI07_RS10650: RPSI07_RS10650 - glutamyl-tRNA reductase, at 593,153 to 594,460
_RS10650
Position (kb) 591
592
593
594 Strain fitness (log2 ratio) -1
0
1 at 591.281 kb on - strand at 591.383 kb on + strand at 591.768 kb on - strand at 591.793 kb on + strand at 591.799 kb on + strand at 591.800 kb on - strand at 591.889 kb on + strand at 591.889 kb on + strand at 591.889 kb on + strand at 591.890 kb on - strand at 591.890 kb on - strand at 591.890 kb on - strand at 591.891 kb on + strand at 591.948 kb on + strand at 591.949 kb on - strand at 593.100 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 591,281 - -1.7 591,383 + -1.4 591,768 - -0.5 591,793 + +0.7 591,799 + -0.0 591,800 - -0.3 591,889 + +1.5 591,889 + -1.4 591,889 + -0.3 591,890 - +1.1 591,890 - -1.3 591,890 - -0.2 591,891 + -0.1 591,948 + -1.3 591,949 - +0.9 593,100 - +0.6
Or see this region's nucleotide sequence