Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS05265 and RPSI07_RS05270 are separated by 78 nucleotides RPSI07_RS05270 and RPSI07_RS05275 are separated by 212 nucleotides
RPSI07_RS05265: RPSI07_RS05265 - MFS transporter, at 1,441,845 to 1,443,158
_RS05265
RPSI07_RS05270: RPSI07_RS05270 - putative hydro-lyase, at 1,443,237 to 1,444,094
_RS05270
RPSI07_RS05275: RPSI07_RS05275 - glutaminase, at 1,444,307 to 1,445,221
_RS05275
Position (kb)
1443
1444
1445 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1442.274 kb on - strand, within RPSI07_RS05265 at 1442.275 kb on + strand, within RPSI07_RS05265 at 1442.275 kb on + strand, within RPSI07_RS05265 at 1442.275 kb on + strand, within RPSI07_RS05265 at 1442.276 kb on - strand, within RPSI07_RS05265 at 1442.276 kb on - strand, within RPSI07_RS05265 at 1442.276 kb on - strand, within RPSI07_RS05265 at 1442.276 kb on - strand, within RPSI07_RS05265 at 1442.276 kb on - strand, within RPSI07_RS05265 at 1442.276 kb on - strand, within RPSI07_RS05265 at 1442.303 kb on - strand, within RPSI07_RS05265 at 1442.374 kb on + strand, within RPSI07_RS05265 at 1442.375 kb on - strand at 1442.620 kb on + strand, within RPSI07_RS05265 at 1442.620 kb on + strand, within RPSI07_RS05265 at 1442.620 kb on + strand, within RPSI07_RS05265 at 1442.621 kb on - strand, within RPSI07_RS05265 at 1442.621 kb on - strand, within RPSI07_RS05265 at 1442.621 kb on - strand, within RPSI07_RS05265 at 1442.621 kb on - strand, within RPSI07_RS05265 at 1442.621 kb on - strand, within RPSI07_RS05265 at 1442.621 kb on - strand, within RPSI07_RS05265 at 1442.621 kb on - strand, within RPSI07_RS05265 at 1442.788 kb on + strand, within RPSI07_RS05265 at 1442.788 kb on + strand, within RPSI07_RS05265 at 1442.789 kb on - strand, within RPSI07_RS05265 at 1443.080 kb on - strand at 1443.152 kb on + strand at 1443.588 kb on + strand, within RPSI07_RS05270 at 1443.589 kb on + strand, within RPSI07_RS05270 at 1443.589 kb on + strand, within RPSI07_RS05270 at 1443.589 kb on + strand, within RPSI07_RS05270 at 1443.589 kb on + strand, within RPSI07_RS05270 at 1443.589 kb on + strand, within RPSI07_RS05270 at 1443.589 kb on + strand, within RPSI07_RS05270 at 1443.590 kb on - strand, within RPSI07_RS05270 at 1443.590 kb on - strand, within RPSI07_RS05270 at 1443.590 kb on - strand, within RPSI07_RS05270 at 1443.928 kb on + strand, within RPSI07_RS05270 at 1443.928 kb on + strand, within RPSI07_RS05270 at 1443.928 kb on + strand, within RPSI07_RS05270 at 1443.928 kb on + strand, within RPSI07_RS05270 at 1443.929 kb on - strand, within RPSI07_RS05270 at 1443.929 kb on - strand, within RPSI07_RS05270 at 1444.086 kb on + strand at 1444.086 kb on + strand at 1444.086 kb on + strand at 1444.087 kb on - strand at 1444.087 kb on - strand at 1444.087 kb on - strand at 1444.095 kb on - strand at 1444.187 kb on - strand at 1444.258 kb on + strand at 1444.258 kb on + strand at 1444.259 kb on - strand at 1444.259 kb on - strand at 1444.291 kb on + strand at 1444.291 kb on + strand at 1444.291 kb on + strand at 1444.292 kb on - strand at 1444.292 kb on - strand at 1444.292 kb on - strand at 1444.292 kb on - strand at 1444.292 kb on - strand at 1444.314 kb on - strand at 1444.314 kb on - strand at 1444.314 kb on - strand at 1444.382 kb on + strand at 1444.382 kb on + strand at 1444.382 kb on + strand at 1444.382 kb on + strand at 1444.383 kb on - strand at 1444.383 kb on - strand at 1444.383 kb on - strand at 1444.751 kb on + strand, within RPSI07_RS05275 at 1444.868 kb on + strand, within RPSI07_RS05275 at 1444.869 kb on - strand, within RPSI07_RS05275 at 1445.025 kb on - strand, within RPSI07_RS05275 at 1445.025 kb on - strand, within RPSI07_RS05275
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 1,442,274 - RPSI07_RS05265 0.33 -2.5 1,442,275 + RPSI07_RS05265 0.33 +0.3 1,442,275 + RPSI07_RS05265 0.33 +1.2 1,442,275 + RPSI07_RS05265 0.33 +0.3 1,442,276 - RPSI07_RS05265 0.33 +1.0 1,442,276 - RPSI07_RS05265 0.33 -1.6 1,442,276 - RPSI07_RS05265 0.33 -0.1 1,442,276 - RPSI07_RS05265 0.33 -0.6 1,442,276 - RPSI07_RS05265 0.33 -0.6 1,442,276 - RPSI07_RS05265 0.33 +0.9 1,442,303 - RPSI07_RS05265 0.35 -3.2 1,442,374 + RPSI07_RS05265 0.40 +0.8 1,442,375 - -1.0 1,442,620 + RPSI07_RS05265 0.59 +0.3 1,442,620 + RPSI07_RS05265 0.59 +0.2 1,442,620 + RPSI07_RS05265 0.59 -0.3 1,442,621 - RPSI07_RS05265 0.59 -0.2 1,442,621 - RPSI07_RS05265 0.59 -0.7 1,442,621 - RPSI07_RS05265 0.59 -0.2 1,442,621 - RPSI07_RS05265 0.59 +2.2 1,442,621 - RPSI07_RS05265 0.59 -0.5 1,442,621 - RPSI07_RS05265 0.59 -2.5 1,442,621 - RPSI07_RS05265 0.59 -0.3 1,442,788 + RPSI07_RS05265 0.72 -2.0 1,442,788 + RPSI07_RS05265 0.72 -1.0 1,442,789 - RPSI07_RS05265 0.72 -0.5 1,443,080 - +0.8 1,443,152 + -0.6 1,443,588 + RPSI07_RS05270 0.41 -1.2 1,443,589 + RPSI07_RS05270 0.41 +0.6 1,443,589 + RPSI07_RS05270 0.41 +0.1 1,443,589 + RPSI07_RS05270 0.41 -1.4 1,443,589 + RPSI07_RS05270 0.41 +0.0 1,443,589 + RPSI07_RS05270 0.41 -1.3 1,443,589 + RPSI07_RS05270 0.41 +0.8 1,443,590 - RPSI07_RS05270 0.41 +0.2 1,443,590 - RPSI07_RS05270 0.41 +1.3 1,443,590 - RPSI07_RS05270 0.41 -0.3 1,443,928 + RPSI07_RS05270 0.81 -2.2 1,443,928 + RPSI07_RS05270 0.81 -0.6 1,443,928 + RPSI07_RS05270 0.81 -0.7 1,443,928 + RPSI07_RS05270 0.81 +2.1 1,443,929 - RPSI07_RS05270 0.81 -0.1 1,443,929 - RPSI07_RS05270 0.81 -0.5 1,444,086 + -1.0 1,444,086 + +0.2 1,444,086 + +0.1 1,444,087 - +0.9 1,444,087 - +0.1 1,444,087 - +1.6 1,444,095 - +0.9 1,444,187 - +2.8 1,444,258 + +0.2 1,444,258 + -1.3 1,444,259 - -1.8 1,444,259 - +1.2 1,444,291 + -1.4 1,444,291 + -0.2 1,444,291 + +0.5 1,444,292 - -0.5 1,444,292 - -1.2 1,444,292 - +1.3 1,444,292 - -0.7 1,444,292 - -0.2 1,444,314 - +1.2 1,444,314 - -0.4 1,444,314 - +0.5 1,444,382 + +1.2 1,444,382 + +1.2 1,444,382 + +2.2 1,444,382 + -0.5 1,444,383 - +1.7 1,444,383 - -0.6 1,444,383 - +0.4 1,444,751 + RPSI07_RS05275 0.49 +0.8 1,444,868 + RPSI07_RS05275 0.61 -0.4 1,444,869 - RPSI07_RS05275 0.61 -0.7 1,445,025 - RPSI07_RS05275 0.78 -0.9 1,445,025 - RPSI07_RS05275 0.78 -0.2
Or see this region's nucleotide sequence