Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS01320 and RPSI07_RS01325 are separated by 796 nucleotides RPSI07_RS01325 and RPSI07_RS01330 are separated by 22 nucleotides
RPSI07_RS01320: RPSI07_RS01320 - 2-isopropylmalate synthase, at 363,399 to 365,129
_RS01320
RPSI07_RS01325: RPSI07_RS01325 - (2Fe-2S)-binding protein, at 365,926 to 366,384
_RS01325
RPSI07_RS01330: RPSI07_RS01330 - xanthine dehydrogenase family protein molybdopterin-binding subunit, at 366,407 to 368,656
_RS01330
Position (kb) 365
366
367 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 365.104 kb on + strand at 365.104 kb on + strand at 365.105 kb on - strand at 365.105 kb on - strand at 365.105 kb on - strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.132 kb on + strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.133 kb on - strand at 365.143 kb on + strand at 365.143 kb on + strand at 365.143 kb on + strand at 365.264 kb on + strand at 365.397 kb on - strand at 365.397 kb on - strand at 365.397 kb on - strand at 365.397 kb on - strand at 365.397 kb on - strand at 365.404 kb on + strand at 365.404 kb on + strand at 365.412 kb on + strand at 365.412 kb on + strand at 365.412 kb on + strand at 365.413 kb on - strand at 365.413 kb on - strand at 365.413 kb on - strand at 365.415 kb on + strand at 365.415 kb on + strand at 365.415 kb on + strand at 365.415 kb on + strand at 365.415 kb on + strand at 365.415 kb on + strand at 365.415 kb on + strand at 365.415 kb on + strand at 365.416 kb on - strand at 365.416 kb on - strand at 365.421 kb on + strand at 365.422 kb on - strand at 365.433 kb on + strand at 365.486 kb on + strand at 365.487 kb on - strand at 365.509 kb on + strand at 365.509 kb on + strand at 365.659 kb on + strand at 365.660 kb on - strand at 365.660 kb on - strand at 365.837 kb on + strand at 365.838 kb on - strand at 365.838 kb on - strand at 365.838 kb on - strand at 365.840 kb on + strand at 365.840 kb on + strand at 365.840 kb on + strand at 365.840 kb on + strand at 365.840 kb on + strand at 365.841 kb on - strand at 365.841 kb on - strand at 365.841 kb on - strand at 365.841 kb on - strand at 365.841 kb on - strand at 365.883 kb on + strand at 365.883 kb on + strand at 365.884 kb on - strand at 365.915 kb on - strand at 365.940 kb on + strand at 365.940 kb on + strand at 365.941 kb on - strand at 366.334 kb on + strand, within RPSI07_RS01325 at 366.334 kb on + strand, within RPSI07_RS01325 at 366.334 kb on + strand, within RPSI07_RS01325 at 366.334 kb on + strand, within RPSI07_RS01325 at 366.334 kb on + strand, within RPSI07_RS01325 at 366.334 kb on + strand, within RPSI07_RS01325 at 366.335 kb on - strand, within RPSI07_RS01325 at 366.335 kb on - strand, within RPSI07_RS01325 at 366.335 kb on - strand, within RPSI07_RS01325 at 366.335 kb on - strand, within RPSI07_RS01325 at 366.383 kb on - strand at 366.383 kb on - strand at 366.689 kb on - strand, within RPSI07_RS01330 at 366.689 kb on - strand, within RPSI07_RS01330 at 366.689 kb on - strand, within RPSI07_RS01330 at 366.689 kb on - strand, within RPSI07_RS01330 at 366.773 kb on + strand, within RPSI07_RS01330 at 366.774 kb on - strand, within RPSI07_RS01330 at 366.842 kb on + strand, within RPSI07_RS01330 at 366.843 kb on - strand, within RPSI07_RS01330 at 366.968 kb on + strand, within RPSI07_RS01330 at 366.968 kb on + strand, within RPSI07_RS01330 at 366.968 kb on + strand, within RPSI07_RS01330 at 366.969 kb on - strand, within RPSI07_RS01330 at 366.969 kb on - strand, within RPSI07_RS01330 at 366.969 kb on - strand, within RPSI07_RS01330 at 367.132 kb on + strand, within RPSI07_RS01330 at 367.133 kb on - strand, within RPSI07_RS01330 at 367.133 kb on - strand, within RPSI07_RS01330 at 367.134 kb on + strand, within RPSI07_RS01330 at 367.187 kb on + strand, within RPSI07_RS01330 at 367.187 kb on + strand, within RPSI07_RS01330 at 367.187 kb on + strand, within RPSI07_RS01330 at 367.376 kb on + strand, within RPSI07_RS01330 at 367.376 kb on + strand, within RPSI07_RS01330 at 367.376 kb on + strand, within RPSI07_RS01330 at 367.376 kb on + strand, within RPSI07_RS01330 at 367.376 kb on + strand, within RPSI07_RS01330 at 367.377 kb on - strand, within RPSI07_RS01330 at 367.377 kb on - strand, within RPSI07_RS01330 at 367.377 kb on - strand, within RPSI07_RS01330 at 367.377 kb on - strand, within RPSI07_RS01330
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 365,104 + +0.7 365,104 + +0.2 365,105 - +0.4 365,105 - +0.4 365,105 - +0.4 365,132 + -1.6 365,132 + -2.0 365,132 + +0.0 365,132 + -1.1 365,132 + -0.4 365,132 + -0.8 365,132 + +0.3 365,132 + +0.6 365,132 + +1.6 365,132 + +5.1 365,132 + +0.2 365,132 + +0.7 365,132 + +0.6 365,133 - +1.7 365,133 - -0.4 365,133 - -0.4 365,133 - -2.4 365,133 - -0.9 365,133 - +0.4 365,133 - -0.2 365,133 - -0.5 365,133 - -1.6 365,133 - +0.4 365,133 - -0.8 365,133 - +0.4 365,133 - +2.8 365,143 + +0.2 365,143 + +0.3 365,143 + -0.5 365,264 + -0.3 365,397 - -0.4 365,397 - -0.6 365,397 - -1.9 365,397 - +1.5 365,397 - -0.3 365,404 + +2.9 365,404 + -0.4 365,412 + -1.5 365,412 + +0.6 365,412 + -0.6 365,413 - -0.2 365,413 - -0.5 365,413 - +0.9 365,415 + +0.8 365,415 + +1.2 365,415 + -0.2 365,415 + +2.2 365,415 + +0.2 365,415 + +0.8 365,415 + +1.2 365,415 + +0.6 365,416 - -1.2 365,416 - +1.3 365,421 + -0.4 365,422 - -1.1 365,433 + +0.7 365,486 + -0.2 365,487 - +0.2 365,509 + -0.9 365,509 + +3.0 365,659 + -0.8 365,660 - +0.5 365,660 - -0.1 365,837 + +0.1 365,838 - +0.1 365,838 - -0.8 365,838 - -0.1 365,840 + -0.8 365,840 + -0.6 365,840 + -1.7 365,840 + -0.6 365,840 + +0.2 365,841 - -0.8 365,841 - +1.2 365,841 - -0.1 365,841 - +0.1 365,841 - -0.3 365,883 + -0.6 365,883 + +1.1 365,884 - +0.3 365,915 - -0.6 365,940 + +0.1 365,940 + +0.1 365,941 - +1.7 366,334 + RPSI07_RS01325 0.89 -0.5 366,334 + RPSI07_RS01325 0.89 +0.2 366,334 + RPSI07_RS01325 0.89 -1.8 366,334 + RPSI07_RS01325 0.89 -1.7 366,334 + RPSI07_RS01325 0.89 -0.5 366,334 + RPSI07_RS01325 0.89 -0.8 366,335 - RPSI07_RS01325 0.89 -0.3 366,335 - RPSI07_RS01325 0.89 +0.4 366,335 - RPSI07_RS01325 0.89 -0.0 366,335 - RPSI07_RS01325 0.89 -0.5 366,383 - -0.5 366,383 - -0.3 366,689 - RPSI07_RS01330 0.13 +0.3 366,689 - RPSI07_RS01330 0.13 -0.7 366,689 - RPSI07_RS01330 0.13 +0.1 366,689 - RPSI07_RS01330 0.13 -1.0 366,773 + RPSI07_RS01330 0.16 -3.2 366,774 - RPSI07_RS01330 0.16 -1.1 366,842 + RPSI07_RS01330 0.19 -1.6 366,843 - RPSI07_RS01330 0.19 -2.0 366,968 + RPSI07_RS01330 0.25 +3.0 366,968 + RPSI07_RS01330 0.25 -0.5 366,968 + RPSI07_RS01330 0.25 -1.3 366,969 - RPSI07_RS01330 0.25 -2.0 366,969 - RPSI07_RS01330 0.25 -0.0 366,969 - RPSI07_RS01330 0.25 +0.2 367,132 + RPSI07_RS01330 0.32 +0.7 367,133 - RPSI07_RS01330 0.32 +0.4 367,133 - RPSI07_RS01330 0.32 +0.9 367,134 + RPSI07_RS01330 0.32 +1.8 367,187 + RPSI07_RS01330 0.35 -1.4 367,187 + RPSI07_RS01330 0.35 +0.5 367,187 + RPSI07_RS01330 0.35 +1.2 367,376 + RPSI07_RS01330 0.43 +1.7 367,376 + RPSI07_RS01330 0.43 +0.3 367,376 + RPSI07_RS01330 0.43 +2.2 367,376 + RPSI07_RS01330 0.43 +0.0 367,376 + RPSI07_RS01330 0.43 -0.8 367,377 - RPSI07_RS01330 0.43 +0.4 367,377 - RPSI07_RS01330 0.43 -0.1 367,377 - RPSI07_RS01330 0.43 -1.0 367,377 - RPSI07_RS01330 0.43 +1.2
Or see this region's nucleotide sequence