Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS00515 and RPSI07_RS00520 are separated by 533 nucleotides RPSI07_RS00520 and RPSI07_RS00525 are separated by 238 nucleotides RPSI07_RS00525 and RPSI07_RS00530 are separated by 149 nucleotides
RPSI07_RS00515: RPSI07_RS00515 - sulfotransferase, at 137,324 to 138,244
_RS00515
RPSI07_RS00520: RPSI07_RS00520 - hypothetical protein, at 138,778 to 139,413
_RS00520
RPSI07_RS00525: RPSI07_RS00525 - hypothetical protein, at 139,652 to 139,999
_RS00525
RPSI07_RS00530: RPSI07_RS00530 - hypothetical protein, at 140,149 to 140,946
_RS00530
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 138.030 kb on + strand, within RPSI07_RS00515 at 138.030 kb on + strand, within RPSI07_RS00515 at 138.031 kb on - strand, within RPSI07_RS00515 at 138.054 kb on - strand, within RPSI07_RS00515 at 138.307 kb on - strand at 138.338 kb on + strand at 138.338 kb on + strand at 138.338 kb on + strand at 138.338 kb on + strand at 138.338 kb on + strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.339 kb on - strand at 138.446 kb on - strand at 138.642 kb on - strand at 138.648 kb on + strand at 138.648 kb on + strand at 138.648 kb on + strand at 138.649 kb on - strand at 138.649 kb on - strand at 138.649 kb on - strand at 138.649 kb on - strand at 138.649 kb on - strand at 138.715 kb on + strand at 138.715 kb on + strand at 138.716 kb on - strand at 138.716 kb on - strand at 138.716 kb on - strand at 138.716 kb on - strand at 138.716 kb on - strand at 138.716 kb on - strand at 138.892 kb on + strand, within RPSI07_RS00520 at 138.892 kb on + strand, within RPSI07_RS00520 at 138.892 kb on + strand, within RPSI07_RS00520 at 138.893 kb on - strand at 138.952 kb on + strand, within RPSI07_RS00520 at 138.952 kb on + strand, within RPSI07_RS00520 at 138.953 kb on - strand, within RPSI07_RS00520 at 139.018 kb on + strand, within RPSI07_RS00520 at 139.019 kb on - strand, within RPSI07_RS00520 at 139.024 kb on + strand, within RPSI07_RS00520 at 139.087 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.120 kb on + strand, within RPSI07_RS00520 at 139.121 kb on - strand, within RPSI07_RS00520 at 139.121 kb on - strand, within RPSI07_RS00520 at 139.121 kb on - strand, within RPSI07_RS00520 at 139.121 kb on - strand, within RPSI07_RS00520 at 139.121 kb on - strand, within RPSI07_RS00520 at 139.186 kb on + strand, within RPSI07_RS00520 at 139.186 kb on + strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.250 kb on - strand, within RPSI07_RS00520 at 139.271 kb on - strand, within RPSI07_RS00520 at 139.271 kb on - strand, within RPSI07_RS00520 at 139.409 kb on + strand at 139.409 kb on + strand at 139.409 kb on + strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.411 kb on + strand at 139.411 kb on + strand at 139.412 kb on - strand at 139.412 kb on - strand at 139.576 kb on + strand at 139.576 kb on + strand at 139.576 kb on + strand at 139.576 kb on + strand at 139.576 kb on + strand at 139.577 kb on - strand at 139.577 kb on - strand at 139.577 kb on - strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.599 kb on - strand at 139.599 kb on - strand at 139.599 kb on - strand at 139.599 kb on - strand at 139.638 kb on + strand at 139.639 kb on - strand at 139.639 kb on - strand at 139.898 kb on + strand, within RPSI07_RS00525 at 139.898 kb on + strand, within RPSI07_RS00525 at 139.899 kb on - strand, within RPSI07_RS00525 at 139.899 kb on - strand, within RPSI07_RS00525 at 139.900 kb on - strand, within RPSI07_RS00525 at 140.297 kb on + strand, within RPSI07_RS00530 at 140.298 kb on - strand, within RPSI07_RS00530 at 140.309 kb on + strand, within RPSI07_RS00530 at 140.310 kb on - strand, within RPSI07_RS00530 at 140.310 kb on - strand, within RPSI07_RS00530
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 138,030 + RPSI07_RS00515 0.77 +0.2 138,030 + RPSI07_RS00515 0.77 +0.5 138,031 - RPSI07_RS00515 0.77 -0.6 138,054 - RPSI07_RS00515 0.79 -0.4 138,307 - -1.8 138,338 + -0.9 138,338 + -0.1 138,338 + -2.4 138,338 + +1.8 138,338 + +0.3 138,339 - +1.4 138,339 - -1.2 138,339 - -0.8 138,339 - +1.7 138,339 - +0.5 138,339 - -0.8 138,339 - -2.5 138,446 - -1.1 138,642 - -1.6 138,648 + -0.6 138,648 + -1.0 138,648 + +0.1 138,649 - +1.2 138,649 - -1.6 138,649 - -1.6 138,649 - +0.7 138,649 - -1.6 138,715 + +0.7 138,715 + -0.7 138,716 - +3.2 138,716 - -1.2 138,716 - -0.9 138,716 - -0.8 138,716 - -1.0 138,716 - -0.3 138,892 + RPSI07_RS00520 0.18 -0.1 138,892 + RPSI07_RS00520 0.18 -0.8 138,892 + RPSI07_RS00520 0.18 -0.1 138,893 - +2.8 138,952 + RPSI07_RS00520 0.27 +0.1 138,952 + RPSI07_RS00520 0.27 +0.3 138,953 - RPSI07_RS00520 0.28 -0.7 139,018 + RPSI07_RS00520 0.38 -1.8 139,019 - RPSI07_RS00520 0.38 +0.2 139,024 + RPSI07_RS00520 0.39 -1.5 139,087 + RPSI07_RS00520 0.49 -1.6 139,120 + RPSI07_RS00520 0.54 +0.2 139,120 + RPSI07_RS00520 0.54 -0.6 139,120 + RPSI07_RS00520 0.54 +0.0 139,120 + RPSI07_RS00520 0.54 +0.6 139,120 + RPSI07_RS00520 0.54 +0.4 139,120 + RPSI07_RS00520 0.54 -0.8 139,120 + RPSI07_RS00520 0.54 -0.7 139,120 + RPSI07_RS00520 0.54 -0.1 139,121 - RPSI07_RS00520 0.54 -0.8 139,121 - RPSI07_RS00520 0.54 -0.3 139,121 - RPSI07_RS00520 0.54 -3.1 139,121 - RPSI07_RS00520 0.54 +0.4 139,121 - RPSI07_RS00520 0.54 +0.6 139,186 + RPSI07_RS00520 0.64 -2.1 139,186 + RPSI07_RS00520 0.64 -0.1 139,187 - RPSI07_RS00520 0.64 -2.4 139,187 - RPSI07_RS00520 0.64 -1.0 139,187 - RPSI07_RS00520 0.64 -1.5 139,187 - RPSI07_RS00520 0.64 -1.1 139,250 - RPSI07_RS00520 0.74 -1.3 139,271 - RPSI07_RS00520 0.78 -1.0 139,271 - RPSI07_RS00520 0.78 +0.5 139,409 + -0.5 139,409 + -0.3 139,409 + +0.6 139,410 - +0.5 139,410 - +1.4 139,410 - +0.1 139,410 - -3.2 139,410 - -0.1 139,410 - -0.0 139,410 - -1.7 139,411 + +0.4 139,411 + +1.2 139,412 - +0.4 139,412 - +0.2 139,576 + +1.1 139,576 + +2.2 139,576 + -1.4 139,576 + -0.1 139,576 + +3.9 139,577 - -0.1 139,577 - -0.4 139,577 - +0.0 139,598 + -2.6 139,598 + -1.4 139,598 + -0.7 139,598 + -0.4 139,598 + +1.1 139,598 + +0.4 139,598 + -0.1 139,598 + -0.4 139,599 - +0.2 139,599 - +1.5 139,599 - +1.7 139,599 - -0.8 139,638 + +1.7 139,639 - +0.9 139,639 - +0.7 139,898 + RPSI07_RS00525 0.71 +1.1 139,898 + RPSI07_RS00525 0.71 -1.0 139,899 - RPSI07_RS00525 0.71 +0.1 139,899 - RPSI07_RS00525 0.71 -2.2 139,900 - RPSI07_RS00525 0.71 +2.2 140,297 + RPSI07_RS00530 0.19 -0.6 140,298 - RPSI07_RS00530 0.19 +0.5 140,309 + RPSI07_RS00530 0.20 +0.2 140,310 - RPSI07_RS00530 0.20 -1.0 140,310 - RPSI07_RS00530 0.20 +0.2
Or see this region's nucleotide sequence