Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21860 and RPSI07_RS21865 are separated by 215 nucleotides RPSI07_RS21865 and RPSI07_RS21870 are separated by 1 nucleotides RPSI07_RS21870 and RPSI07_RS21875 are separated by 286 nucleotides
RPSI07_RS21860: RPSI07_RS21860 - alpha/beta hydrolase, at 3,066,735 to 3,067,688
_RS21860
RPSI07_RS21865: RPSI07_RS21865 - transporter component, transmembrane domains, at 3,067,904 to 3,068,338
_RS21865
RPSI07_RS21870: RPSI07_RS21870 - membrane protein, at 3,068,340 to 3,068,753
_RS21870
RPSI07_RS21875: RPSI07_RS21875 - FAA hydrolase family protein, at 3,069,040 to 3,069,888
_RS21875
Position (kb)
3068
3069 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3067.450 kb on + strand, within RPSI07_RS21860 at 3067.450 kb on + strand, within RPSI07_RS21860 at 3067.451 kb on - strand, within RPSI07_RS21860 at 3067.451 kb on - strand, within RPSI07_RS21860 at 3067.451 kb on - strand, within RPSI07_RS21860 at 3067.451 kb on - strand, within RPSI07_RS21860 at 3067.451 kb on - strand, within RPSI07_RS21860 at 3067.458 kb on + strand, within RPSI07_RS21860 at 3067.458 kb on + strand, within RPSI07_RS21860 at 3067.459 kb on - strand, within RPSI07_RS21860 at 3067.719 kb on + strand at 3067.719 kb on + strand at 3067.719 kb on + strand at 3067.837 kb on + strand at 3067.837 kb on + strand at 3067.837 kb on + strand at 3067.837 kb on + strand at 3067.863 kb on + strand at 3067.864 kb on - strand at 3067.881 kb on + strand at 3067.882 kb on - strand at 3067.882 kb on - strand at 3068.232 kb on + strand, within RPSI07_RS21865 at 3068.232 kb on + strand, within RPSI07_RS21865 at 3068.233 kb on - strand, within RPSI07_RS21865 at 3068.706 kb on - strand, within RPSI07_RS21870 at 3068.752 kb on - strand at 3068.752 kb on - strand at 3069.050 kb on + strand at 3069.056 kb on + strand at 3069.056 kb on + strand at 3069.057 kb on - strand at 3069.057 kb on - strand at 3069.057 kb on - strand at 3069.057 kb on - strand at 3069.125 kb on + strand, within RPSI07_RS21875 at 3069.125 kb on + strand, within RPSI07_RS21875 at 3069.274 kb on + strand, within RPSI07_RS21875 at 3069.275 kb on - strand, within RPSI07_RS21875 at 3069.275 kb on - strand, within RPSI07_RS21875 at 3069.503 kb on + strand, within RPSI07_RS21875 at 3069.503 kb on + strand, within RPSI07_RS21875 at 3069.503 kb on + strand, within RPSI07_RS21875 at 3069.503 kb on + strand, within RPSI07_RS21875 at 3069.504 kb on - strand, within RPSI07_RS21875 at 3069.672 kb on + strand, within RPSI07_RS21875 at 3069.712 kb on + strand, within RPSI07_RS21875 at 3069.712 kb on + strand, within RPSI07_RS21875 at 3069.712 kb on + strand, within RPSI07_RS21875 at 3069.712 kb on + strand, within RPSI07_RS21875 at 3069.712 kb on + strand, within RPSI07_RS21875 at 3069.712 kb on + strand, within RPSI07_RS21875 at 3069.712 kb on + strand, within RPSI07_RS21875 at 3069.712 kb on + strand, within RPSI07_RS21875 at 3069.713 kb on - strand, within RPSI07_RS21875 at 3069.713 kb on - strand, within RPSI07_RS21875 at 3069.713 kb on - strand, within RPSI07_RS21875 at 3069.713 kb on - strand, within RPSI07_RS21875 at 3069.713 kb on - strand, within RPSI07_RS21875 at 3069.713 kb on - strand, within RPSI07_RS21875 at 3069.713 kb on - strand, within RPSI07_RS21875 at 3069.713 kb on - strand, within RPSI07_RS21875 at 3069.713 kb on - strand, within RPSI07_RS21875
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 3,067,450 + RPSI07_RS21860 0.75 +0.1 3,067,450 + RPSI07_RS21860 0.75 -0.0 3,067,451 - RPSI07_RS21860 0.75 +2.9 3,067,451 - RPSI07_RS21860 0.75 -0.1 3,067,451 - RPSI07_RS21860 0.75 +0.4 3,067,451 - RPSI07_RS21860 0.75 -1.6 3,067,451 - RPSI07_RS21860 0.75 -0.2 3,067,458 + RPSI07_RS21860 0.76 +0.3 3,067,458 + RPSI07_RS21860 0.76 +1.1 3,067,459 - RPSI07_RS21860 0.76 -1.5 3,067,719 + -1.0 3,067,719 + -0.8 3,067,719 + -0.6 3,067,837 + -0.2 3,067,837 + +0.5 3,067,837 + -0.1 3,067,837 + +1.3 3,067,863 + -0.8 3,067,864 - -0.8 3,067,881 + -0.7 3,067,882 - +0.7 3,067,882 - -0.6 3,068,232 + RPSI07_RS21865 0.75 +0.0 3,068,232 + RPSI07_RS21865 0.75 +0.3 3,068,233 - RPSI07_RS21865 0.76 -0.0 3,068,706 - RPSI07_RS21870 0.88 +1.3 3,068,752 - +0.4 3,068,752 - -1.5 3,069,050 + -0.5 3,069,056 + -0.3 3,069,056 + +3.2 3,069,057 - -0.3 3,069,057 - -0.2 3,069,057 - +0.3 3,069,057 - +0.6 3,069,125 + RPSI07_RS21875 0.10 -0.0 3,069,125 + RPSI07_RS21875 0.10 -0.3 3,069,274 + RPSI07_RS21875 0.28 -1.4 3,069,275 - RPSI07_RS21875 0.28 -4.3 3,069,275 - RPSI07_RS21875 0.28 -0.9 3,069,503 + RPSI07_RS21875 0.55 -1.2 3,069,503 + RPSI07_RS21875 0.55 -1.0 3,069,503 + RPSI07_RS21875 0.55 -0.1 3,069,503 + RPSI07_RS21875 0.55 +1.4 3,069,504 - RPSI07_RS21875 0.55 -2.0 3,069,672 + RPSI07_RS21875 0.74 -1.6 3,069,712 + RPSI07_RS21875 0.79 -1.0 3,069,712 + RPSI07_RS21875 0.79 -0.8 3,069,712 + RPSI07_RS21875 0.79 +1.0 3,069,712 + RPSI07_RS21875 0.79 -0.3 3,069,712 + RPSI07_RS21875 0.79 -0.3 3,069,712 + RPSI07_RS21875 0.79 +2.9 3,069,712 + RPSI07_RS21875 0.79 -0.0 3,069,712 + RPSI07_RS21875 0.79 -0.7 3,069,713 - RPSI07_RS21875 0.79 +2.9 3,069,713 - RPSI07_RS21875 0.79 -0.8 3,069,713 - RPSI07_RS21875 0.79 -0.8 3,069,713 - RPSI07_RS21875 0.79 +0.2 3,069,713 - RPSI07_RS21875 0.79 +0.8 3,069,713 - RPSI07_RS21875 0.79 -0.3 3,069,713 - RPSI07_RS21875 0.79 -0.2 3,069,713 - RPSI07_RS21875 0.79 +0.1 3,069,713 - RPSI07_RS21875 0.79 -0.5
Or see this region's nucleotide sequence