Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS18950 and RPSI07_RS18955 overlap by 4 nucleotides RPSI07_RS18955 and RPSI07_RS18960 overlap by 4 nucleotides RPSI07_RS18960 and RPSI07_RS18965 overlap by 4 nucleotides RPSI07_RS18965 and RPSI07_RS18970 are separated by 20 nucleotides
RPSI07_RS18950: RPSI07_RS18950 - beta-N-acetylhexosaminidase, at 2,444,047 to 2,445,099
_RS18950
RPSI07_RS18955: RPSI07_RS18955 - holo-ACP synthase, at 2,445,096 to 2,445,539
_RS18955
RPSI07_RS18960: RPSI07_RS18960 - pyridoxine 5'-phosphate synthase, at 2,445,536 to 2,446,327
_RS18960
RPSI07_RS18965: RPSI07_RS18965 - DNA repair protein RecO, at 2,446,324 to 2,447,142
_RS18965
RPSI07_RS18970: RPSI07_RS18970 - GTPase Era, at 2,447,163 to 2,448,101
_RS18970
Position (kb)
2445
2446
2447 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2446.328 kb on - strand at 2446.328 kb on - strand at 2446.386 kb on - strand at 2446.442 kb on + strand, within RPSI07_RS18965 at 2446.541 kb on + strand, within RPSI07_RS18965 at 2446.541 kb on + strand, within RPSI07_RS18965 at 2446.542 kb on - strand, within RPSI07_RS18965 at 2446.542 kb on - strand, within RPSI07_RS18965 at 2446.547 kb on + strand, within RPSI07_RS18965 at 2446.648 kb on - strand, within RPSI07_RS18965 at 2446.994 kb on + strand, within RPSI07_RS18965 at 2446.994 kb on + strand, within RPSI07_RS18965 at 2446.994 kb on + strand, within RPSI07_RS18965 at 2446.994 kb on + strand, within RPSI07_RS18965 at 2446.994 kb on + strand, within RPSI07_RS18965 at 2447.164 kb on + strand at 2447.165 kb on - strand at 2447.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 2,446,328 - -0.2 2,446,328 - +1.4 2,446,386 - -0.3 2,446,442 + RPSI07_RS18965 0.14 -0.6 2,446,541 + RPSI07_RS18965 0.26 -0.9 2,446,541 + RPSI07_RS18965 0.26 -4.0 2,446,542 - RPSI07_RS18965 0.27 -1.9 2,446,542 - RPSI07_RS18965 0.27 -1.0 2,446,547 + RPSI07_RS18965 0.27 +1.0 2,446,648 - RPSI07_RS18965 0.40 +1.6 2,446,994 + RPSI07_RS18965 0.82 +0.1 2,446,994 + RPSI07_RS18965 0.82 -0.9 2,446,994 + RPSI07_RS18965 0.82 +0.1 2,446,994 + RPSI07_RS18965 0.82 -0.8 2,446,994 + RPSI07_RS18965 0.82 -2.0 2,447,164 + -1.2 2,447,165 - -1.0 2,447,177 - -0.7
Or see this region's nucleotide sequence