Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS18960

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS18950 and RPSI07_RS18955 overlap by 4 nucleotidesRPSI07_RS18955 and RPSI07_RS18960 overlap by 4 nucleotidesRPSI07_RS18960 and RPSI07_RS18965 overlap by 4 nucleotidesRPSI07_RS18965 and RPSI07_RS18970 are separated by 20 nucleotides RPSI07_RS18950: RPSI07_RS18950 - beta-N-acetylhexosaminidase, at 2,444,047 to 2,445,099 _RS18950 RPSI07_RS18955: RPSI07_RS18955 - holo-ACP synthase, at 2,445,096 to 2,445,539 _RS18955 RPSI07_RS18960: RPSI07_RS18960 - pyridoxine 5'-phosphate synthase, at 2,445,536 to 2,446,327 _RS18960 RPSI07_RS18965: RPSI07_RS18965 - DNA repair protein RecO, at 2,446,324 to 2,447,142 _RS18965 RPSI07_RS18970: RPSI07_RS18970 - GTPase Era, at 2,447,163 to 2,448,101 _RS18970 Position (kb) 2445 2446 2447Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2446.328 kb on - strandat 2446.328 kb on - strandat 2446.386 kb on - strandat 2446.442 kb on + strand, within RPSI07_RS18965at 2446.541 kb on + strand, within RPSI07_RS18965at 2446.541 kb on + strand, within RPSI07_RS18965at 2446.542 kb on - strand, within RPSI07_RS18965at 2446.542 kb on - strand, within RPSI07_RS18965at 2446.547 kb on + strand, within RPSI07_RS18965at 2446.648 kb on - strand, within RPSI07_RS18965at 2446.994 kb on + strand, within RPSI07_RS18965at 2446.994 kb on + strand, within RPSI07_RS18965at 2446.994 kb on + strand, within RPSI07_RS18965at 2446.994 kb on + strand, within RPSI07_RS18965at 2446.994 kb on + strand, within RPSI07_RS18965at 2447.164 kb on + strandat 2447.165 kb on - strandat 2447.177 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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2,446,328 - -0.2
2,446,328 - +1.4
2,446,386 - -0.3
2,446,442 + RPSI07_RS18965 0.14 -0.6
2,446,541 + RPSI07_RS18965 0.26 -0.9
2,446,541 + RPSI07_RS18965 0.26 -4.0
2,446,542 - RPSI07_RS18965 0.27 -1.9
2,446,542 - RPSI07_RS18965 0.27 -1.0
2,446,547 + RPSI07_RS18965 0.27 +1.0
2,446,648 - RPSI07_RS18965 0.40 +1.6
2,446,994 + RPSI07_RS18965 0.82 +0.1
2,446,994 + RPSI07_RS18965 0.82 -0.9
2,446,994 + RPSI07_RS18965 0.82 +0.1
2,446,994 + RPSI07_RS18965 0.82 -0.8
2,446,994 + RPSI07_RS18965 0.82 -2.0
2,447,164 + -1.2
2,447,165 - -1.0
2,447,177 - -0.7

Or see this region's nucleotide sequence