Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS18445 and RPSI07_RS18450 are separated by 55 nucleotides RPSI07_RS18450 and RPSI07_RS18455 are separated by 11 nucleotides RPSI07_RS18455 and RPSI07_RS25170 are separated by 226 nucleotides RPSI07_RS25170 and RPSI07_RS18460 are separated by 135 nucleotides
RPSI07_RS18445: RPSI07_RS18445 - hypothetical protein, at 2,340,952 to 2,342,019
_RS18445
RPSI07_RS18450: RPSI07_RS18450 - peptidylprolyl isomerase, at 2,342,075 to 2,342,599
_RS18450
RPSI07_RS18455: RPSI07_RS18455 - cupin domain-containing protein, at 2,342,611 to 2,343,840
_RS18455
RPSI07_RS25170: RPSI07_RS25170 - hypothetical protein, at 2,344,067 to 2,344,339
_RS25170
RPSI07_RS18460: RPSI07_RS18460 - hypothetical protein, at 2,344,475 to 2,345,260
_RS18460
Position (kb)
2342
2343
2344 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2341.742 kb on - strand, within RPSI07_RS18445 at 2341.797 kb on - strand, within RPSI07_RS18445 at 2342.053 kb on + strand at 2342.053 kb on + strand at 2342.061 kb on - strand at 2342.277 kb on + strand, within RPSI07_RS18450 at 2342.391 kb on + strand, within RPSI07_RS18450 at 2342.505 kb on + strand, within RPSI07_RS18450 at 2342.506 kb on - strand, within RPSI07_RS18450 at 2342.506 kb on - strand at 2342.560 kb on + strand at 2342.560 kb on + strand at 2342.560 kb on + strand at 2342.561 kb on - strand at 2342.562 kb on + strand at 2342.582 kb on - strand at 2342.628 kb on - strand at 2342.629 kb on + strand at 2343.041 kb on - strand, within RPSI07_RS18455 at 2343.232 kb on + strand, within RPSI07_RS18455 at 2343.247 kb on + strand, within RPSI07_RS18455 at 2343.669 kb on + strand, within RPSI07_RS18455 at 2343.797 kb on - strand at 2343.895 kb on - strand at 2343.903 kb on + strand at 2343.904 kb on - strand at 2343.908 kb on - strand at 2343.930 kb on + strand at 2343.931 kb on - strand at 2343.977 kb on + strand at 2343.977 kb on + strand at 2343.978 kb on - strand at 2343.994 kb on + strand at 2344.013 kb on - strand at 2344.013 kb on - strand at 2344.017 kb on - strand at 2344.049 kb on + strand at 2344.337 kb on + strand at 2344.455 kb on - strand at 2344.716 kb on + strand, within RPSI07_RS18460 at 2344.716 kb on + strand, within RPSI07_RS18460
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 2,341,742 - RPSI07_RS18445 0.74 +0.5 2,341,797 - RPSI07_RS18445 0.79 -0.6 2,342,053 + -1.3 2,342,053 + +1.4 2,342,061 - +2.1 2,342,277 + RPSI07_RS18450 0.38 -0.2 2,342,391 + RPSI07_RS18450 0.60 +2.0 2,342,505 + RPSI07_RS18450 0.82 -1.3 2,342,506 - RPSI07_RS18450 0.82 -0.9 2,342,506 - -1.2 2,342,560 + -1.0 2,342,560 + +0.7 2,342,560 + +0.5 2,342,561 - -0.3 2,342,562 + +2.1 2,342,582 - -1.6 2,342,628 - +1.1 2,342,629 + -0.6 2,343,041 - RPSI07_RS18455 0.35 +0.1 2,343,232 + RPSI07_RS18455 0.50 -1.2 2,343,247 + RPSI07_RS18455 0.52 -0.2 2,343,669 + RPSI07_RS18455 0.86 -1.2 2,343,797 - -1.7 2,343,895 - -2.6 2,343,903 + +0.2 2,343,904 - +0.4 2,343,908 - -0.7 2,343,930 + +0.8 2,343,931 - +1.1 2,343,977 + +1.0 2,343,977 + -0.7 2,343,978 - +0.4 2,343,994 + +0.4 2,344,013 - -2.1 2,344,013 - +0.1 2,344,017 - -0.7 2,344,049 + +0.8 2,344,337 + -1.9 2,344,455 - +0.7 2,344,716 + RPSI07_RS18460 0.31 -0.1 2,344,716 + RPSI07_RS18460 0.31 -0.9
Or see this region's nucleotide sequence