Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16235 and RPSI07_RS16240 are separated by 79 nucleotides RPSI07_RS16240 and RPSI07_RS16245 are separated by 472 nucleotides
RPSI07_RS16235: RPSI07_RS16235 - ABC transporter substrate-binding protein, at 1,802,477 to 1,803,517
_RS16235
RPSI07_RS16240: RPSI07_RS16240 - ABC transporter ATP-binding protein, at 1,803,597 to 1,804,688
_RS16240
RPSI07_RS16245: RPSI07_RS16245 - sensor histidine kinase, at 1,805,161 to 1,806,576
_RS16245
Position (kb)
1803
1804
1805 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1802.643 kb on + strand, within RPSI07_RS16235 at 1802.643 kb on + strand, within RPSI07_RS16235 at 1802.661 kb on + strand, within RPSI07_RS16235 at 1802.661 kb on + strand, within RPSI07_RS16235 at 1802.691 kb on + strand, within RPSI07_RS16235 at 1802.691 kb on + strand, within RPSI07_RS16235 at 1802.691 kb on + strand, within RPSI07_RS16235 at 1802.700 kb on + strand, within RPSI07_RS16235 at 1802.700 kb on + strand, within RPSI07_RS16235 at 1802.700 kb on + strand, within RPSI07_RS16235 at 1802.701 kb on - strand, within RPSI07_RS16235 at 1802.748 kb on + strand, within RPSI07_RS16235 at 1802.749 kb on - strand, within RPSI07_RS16235 at 1802.749 kb on - strand, within RPSI07_RS16235 at 1802.761 kb on - strand, within RPSI07_RS16235 at 1802.761 kb on - strand, within RPSI07_RS16235 at 1802.761 kb on - strand, within RPSI07_RS16235 at 1802.904 kb on + strand, within RPSI07_RS16235 at 1802.904 kb on + strand, within RPSI07_RS16235 at 1802.905 kb on - strand, within RPSI07_RS16235 at 1802.970 kb on + strand, within RPSI07_RS16235 at 1802.970 kb on + strand, within RPSI07_RS16235 at 1802.970 kb on + strand, within RPSI07_RS16235 at 1802.971 kb on - strand, within RPSI07_RS16235 at 1802.971 kb on - strand, within RPSI07_RS16235 at 1802.971 kb on - strand, within RPSI07_RS16235 at 1802.971 kb on - strand, within RPSI07_RS16235 at 1803.022 kb on - strand, within RPSI07_RS16235 at 1803.022 kb on - strand, within RPSI07_RS16235 at 1803.110 kb on - strand, within RPSI07_RS16235 at 1803.121 kb on - strand, within RPSI07_RS16235 at 1803.337 kb on - strand, within RPSI07_RS16235 at 1803.337 kb on - strand, within RPSI07_RS16235 at 1803.337 kb on - strand, within RPSI07_RS16235 at 1803.752 kb on - strand, within RPSI07_RS16240 at 1803.752 kb on - strand, within RPSI07_RS16240 at 1803.752 kb on - strand, within RPSI07_RS16240 at 1804.010 kb on - strand, within RPSI07_RS16240 at 1804.108 kb on + strand, within RPSI07_RS16240 at 1804.108 kb on + strand, within RPSI07_RS16240 at 1804.108 kb on + strand, within RPSI07_RS16240 at 1804.109 kb on - strand, within RPSI07_RS16240 at 1804.154 kb on - strand, within RPSI07_RS16240 at 1804.382 kb on - strand, within RPSI07_RS16240 at 1804.423 kb on + strand, within RPSI07_RS16240 at 1804.424 kb on - strand, within RPSI07_RS16240 at 1804.424 kb on - strand, within RPSI07_RS16240 at 1804.594 kb on + strand at 1804.594 kb on + strand at 1804.595 kb on - strand at 1804.595 kb on - strand at 1804.639 kb on + strand at 1804.639 kb on + strand at 1804.640 kb on - strand at 1804.640 kb on - strand at 1804.650 kb on + strand at 1804.650 kb on + strand at 1804.651 kb on - strand at 1804.651 kb on - strand at 1804.762 kb on + strand at 1804.762 kb on + strand at 1804.762 kb on + strand at 1804.762 kb on + strand at 1804.762 kb on + strand at 1804.763 kb on - strand at 1804.763 kb on - strand at 1804.763 kb on - strand at 1804.873 kb on + strand at 1804.874 kb on - strand at 1804.948 kb on - strand at 1805.651 kb on + strand, within RPSI07_RS16245 at 1805.651 kb on + strand, within RPSI07_RS16245 at 1805.652 kb on - strand, within RPSI07_RS16245 at 1805.652 kb on - strand, within RPSI07_RS16245 at 1805.652 kb on - strand, within RPSI07_RS16245
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 1,802,643 + RPSI07_RS16235 0.16 +1.3 1,802,643 + RPSI07_RS16235 0.16 +0.4 1,802,661 + RPSI07_RS16235 0.18 -0.0 1,802,661 + RPSI07_RS16235 0.18 +0.1 1,802,691 + RPSI07_RS16235 0.21 +0.3 1,802,691 + RPSI07_RS16235 0.21 -1.0 1,802,691 + RPSI07_RS16235 0.21 +1.3 1,802,700 + RPSI07_RS16235 0.21 -1.0 1,802,700 + RPSI07_RS16235 0.21 -0.5 1,802,700 + RPSI07_RS16235 0.21 -0.4 1,802,701 - RPSI07_RS16235 0.22 -0.0 1,802,748 + RPSI07_RS16235 0.26 +1.3 1,802,749 - RPSI07_RS16235 0.26 -0.8 1,802,749 - RPSI07_RS16235 0.26 -0.8 1,802,761 - RPSI07_RS16235 0.27 -0.1 1,802,761 - RPSI07_RS16235 0.27 -1.2 1,802,761 - RPSI07_RS16235 0.27 -0.3 1,802,904 + RPSI07_RS16235 0.41 +0.3 1,802,904 + RPSI07_RS16235 0.41 -1.5 1,802,905 - RPSI07_RS16235 0.41 -1.4 1,802,970 + RPSI07_RS16235 0.47 -0.0 1,802,970 + RPSI07_RS16235 0.47 -0.6 1,802,970 + RPSI07_RS16235 0.47 -0.8 1,802,971 - RPSI07_RS16235 0.47 +0.2 1,802,971 - RPSI07_RS16235 0.47 +0.5 1,802,971 - RPSI07_RS16235 0.47 -1.7 1,802,971 - RPSI07_RS16235 0.47 -2.9 1,803,022 - RPSI07_RS16235 0.52 -0.6 1,803,022 - RPSI07_RS16235 0.52 -0.0 1,803,110 - RPSI07_RS16235 0.61 +2.0 1,803,121 - RPSI07_RS16235 0.62 -1.0 1,803,337 - RPSI07_RS16235 0.83 -1.0 1,803,337 - RPSI07_RS16235 0.83 -1.8 1,803,337 - RPSI07_RS16235 0.83 -2.8 1,803,752 - RPSI07_RS16240 0.14 -0.6 1,803,752 - RPSI07_RS16240 0.14 +1.0 1,803,752 - RPSI07_RS16240 0.14 -1.9 1,804,010 - RPSI07_RS16240 0.38 -0.5 1,804,108 + RPSI07_RS16240 0.47 -2.8 1,804,108 + RPSI07_RS16240 0.47 -0.8 1,804,108 + RPSI07_RS16240 0.47 -1.2 1,804,109 - RPSI07_RS16240 0.47 -0.1 1,804,154 - RPSI07_RS16240 0.51 -1.6 1,804,382 - RPSI07_RS16240 0.72 -0.5 1,804,423 + RPSI07_RS16240 0.76 +0.8 1,804,424 - RPSI07_RS16240 0.76 -0.8 1,804,424 - RPSI07_RS16240 0.76 +2.3 1,804,594 + +0.3 1,804,594 + -1.0 1,804,595 - -0.2 1,804,595 - -0.1 1,804,639 + +0.8 1,804,639 + -0.4 1,804,640 - +3.7 1,804,640 - +4.0 1,804,650 + -0.7 1,804,650 + -0.4 1,804,651 - -0.4 1,804,651 - +1.2 1,804,762 + +0.3 1,804,762 + -0.1 1,804,762 + -0.6 1,804,762 + -1.1 1,804,762 + -0.4 1,804,763 - -0.6 1,804,763 - +1.4 1,804,763 - +1.3 1,804,873 + +0.3 1,804,874 - +3.7 1,804,948 - -1.3 1,805,651 + RPSI07_RS16245 0.35 +0.7 1,805,651 + RPSI07_RS16245 0.35 +0.3 1,805,652 - RPSI07_RS16245 0.35 -0.4 1,805,652 - RPSI07_RS16245 0.35 -1.0 1,805,652 - RPSI07_RS16245 0.35 +0.2
Or see this region's nucleotide sequence