Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS13475

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS13465 and RPSI07_RS13470 are separated by 85 nucleotidesRPSI07_RS13470 and RPSI07_RS13475 are separated by 82 nucleotidesRPSI07_RS13475 and RPSI07_RS13480 are separated by 4 nucleotides RPSI07_RS13465: RPSI07_RS13465 - homoserine kinase, at 1,210,886 to 1,211,890 _RS13465 RPSI07_RS13470: RPSI07_RS13470 - membrane protein, at 1,211,976 to 1,212,404 _RS13470 RPSI07_RS13475: RPSI07_RS13475 - TIGR00730 family Rossman fold protein, at 1,212,487 to 1,213,449 _RS13475 RPSI07_RS13480: RPSI07_RS13480 - DNA polymerase I, at 1,213,454 to 1,216,288 _RS13480 Position (kb) 1212 1213 1214Strain fitness (log2 ratio) -3 -2 -1 0 1at 1212.440 kb on + strandat 1212.488 kb on + strandat 1212.488 kb on + strandat 1212.488 kb on + strandat 1212.495 kb on - strandat 1212.545 kb on + strandat 1212.555 kb on - strandat 1213.025 kb on + strand, within RPSI07_RS13475at 1213.026 kb on - strand, within RPSI07_RS13475at 1213.094 kb on + strand, within RPSI07_RS13475at 1213.156 kb on - strand, within RPSI07_RS13475at 1214.381 kb on + strand, within RPSI07_RS13480at 1214.381 kb on + strand, within RPSI07_RS13480

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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1,212,440 + +0.1
1,212,488 + +1.1
1,212,488 + +0.1
1,212,488 + -0.5
1,212,495 - +1.4
1,212,545 + -0.0
1,212,555 - -0.4
1,213,025 + RPSI07_RS13475 0.56 +1.4
1,213,026 - RPSI07_RS13475 0.56 -1.7
1,213,094 + RPSI07_RS13475 0.63 -0.6
1,213,156 - RPSI07_RS13475 0.69 -1.9
1,214,381 + RPSI07_RS13480 0.33 -2.9
1,214,381 + RPSI07_RS13480 0.33 -0.2

Or see this region's nucleotide sequence