Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS00530 and RPSI07_RS00535 are separated by 326 nucleotides RPSI07_RS00535 and RPSI07_RS00540 are separated by 570 nucleotides
RPSI07_RS00530: RPSI07_RS00530 - hypothetical protein, at 140,149 to 140,946
_RS00530
RPSI07_RS00535: RPSI07_RS00535 - hypothetical protein, at 141,273 to 141,563
_RS00535
RPSI07_RS00540: RPSI07_RS00540 - type VI secretion protein, at 142,134 to 145,016
_RS00540
Position (kb) 141
142 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 140.297 kb on + strand, within RPSI07_RS00530 at 140.298 kb on - strand, within RPSI07_RS00530 at 140.309 kb on + strand, within RPSI07_RS00530 at 140.310 kb on - strand, within RPSI07_RS00530 at 140.310 kb on - strand, within RPSI07_RS00530 at 140.514 kb on - strand, within RPSI07_RS00530 at 140.514 kb on - strand, within RPSI07_RS00530 at 140.645 kb on - strand, within RPSI07_RS00530 at 140.645 kb on - strand, within RPSI07_RS00530 at 140.849 kb on + strand, within RPSI07_RS00530 at 140.913 kb on - strand at 140.913 kb on - strand at 140.913 kb on - strand at 140.988 kb on - strand at 141.157 kb on + strand at 141.157 kb on + strand at 141.158 kb on - strand at 141.245 kb on - strand at 141.245 kb on - strand at 141.245 kb on - strand at 141.285 kb on + strand at 141.285 kb on + strand at 141.285 kb on + strand at 141.285 kb on + strand at 141.285 kb on + strand at 141.286 kb on - strand at 141.286 kb on - strand at 141.286 kb on - strand at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.664 kb on + strand at 141.664 kb on + strand at 141.706 kb on + strand at 141.706 kb on + strand at 141.706 kb on + strand at 141.706 kb on + strand at 141.706 kb on + strand at 141.707 kb on - strand at 141.707 kb on - strand at 141.707 kb on - strand at 141.707 kb on - strand at 141.728 kb on + strand at 141.728 kb on + strand at 141.728 kb on + strand at 141.733 kb on + strand at 141.771 kb on - strand at 141.771 kb on - strand at 141.972 kb on - strand at 142.003 kb on + strand at 142.004 kb on - strand at 142.004 kb on - strand at 142.004 kb on - strand at 142.019 kb on + strand at 142.063 kb on + strand at 142.064 kb on - strand at 142.064 kb on - strand at 142.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 140,297 + RPSI07_RS00530 0.19 -0.6 140,298 - RPSI07_RS00530 0.19 +0.3 140,309 + RPSI07_RS00530 0.20 -0.7 140,310 - RPSI07_RS00530 0.20 -0.9 140,310 - RPSI07_RS00530 0.20 +0.3 140,514 - RPSI07_RS00530 0.46 -2.3 140,514 - RPSI07_RS00530 0.46 -1.4 140,645 - RPSI07_RS00530 0.62 +0.0 140,645 - RPSI07_RS00530 0.62 +1.6 140,849 + RPSI07_RS00530 0.88 -3.1 140,913 - -1.0 140,913 - -0.5 140,913 - +0.0 140,988 - -0.8 141,157 + +1.0 141,157 + +0.4 141,158 - -0.1 141,245 - -0.6 141,245 - -1.6 141,245 - +0.6 141,285 + +1.0 141,285 + -0.3 141,285 + -0.4 141,285 + -0.2 141,285 + +0.3 141,286 - -1.0 141,286 - +0.1 141,286 - +1.0 141,512 + RPSI07_RS00535 0.82 +0.6 141,512 + RPSI07_RS00535 0.82 -0.5 141,512 + RPSI07_RS00535 0.82 -0.6 141,512 + RPSI07_RS00535 0.82 +2.0 141,512 + RPSI07_RS00535 0.82 +4.5 141,512 + RPSI07_RS00535 0.82 -0.0 141,512 + RPSI07_RS00535 0.82 +0.4 141,513 - RPSI07_RS00535 0.82 -2.1 141,513 - RPSI07_RS00535 0.82 -1.4 141,513 - RPSI07_RS00535 0.82 +0.2 141,513 - RPSI07_RS00535 0.82 -0.1 141,513 - RPSI07_RS00535 0.82 -0.2 141,513 - RPSI07_RS00535 0.82 +0.2 141,664 + -1.2 141,664 + -0.0 141,706 + -0.7 141,706 + -0.7 141,706 + -0.7 141,706 + -1.4 141,706 + -0.4 141,707 - +1.0 141,707 - -0.6 141,707 - -0.2 141,707 - -0.3 141,728 + -1.9 141,728 + -1.2 141,728 + -0.9 141,733 + +0.5 141,771 - +1.1 141,771 - -0.7 141,972 - +0.4 142,003 + +0.8 142,004 - +0.2 142,004 - +0.1 142,004 - -1.4 142,019 + +0.3 142,063 + +0.2 142,064 - +1.9 142,064 - +0.4 142,105 + +0.5
Or see this region's nucleotide sequence