Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS06885 and RPSI07_RS06890 are separated by 22 nucleotides RPSI07_RS06890 and RPSI07_RS24675 are separated by 163 nucleotides RPSI07_RS24675 and RPSI07_RS06895 are separated by 841 nucleotides
RPSI07_RS06885: RPSI07_RS06885 - adenylosuccinate synthetase, at 1,833,944 to 1,835,659
_RS06885
RPSI07_RS06890: RPSI07_RS06890 - hypothetical protein, at 1,835,682 to 1,836,293
_RS06890
RPSI07_RS24675: RPSI07_RS24675 - hypothetical protein, at 1,836,457 to 1,836,795
_RS24675
RPSI07_RS06895: RPSI07_RS06895 - AraC family transcriptional regulator, at 1,837,637 to 1,838,656
_RS06895
Position (kb)
1836
1837 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1835.473 kb on - strand, within RPSI07_RS06885 at 1835.473 kb on - strand, within RPSI07_RS06885 at 1835.473 kb on - strand, within RPSI07_RS06885 at 1835.664 kb on + strand at 1835.666 kb on - strand at 1835.705 kb on + strand at 1835.706 kb on - strand at 1835.706 kb on - strand at 1835.709 kb on - strand at 1835.709 kb on - strand at 1835.784 kb on + strand, within RPSI07_RS06890 at 1835.784 kb on + strand, within RPSI07_RS06890 at 1835.784 kb on + strand, within RPSI07_RS06890 at 1835.785 kb on - strand, within RPSI07_RS06890 at 1835.785 kb on - strand, within RPSI07_RS06890 at 1835.785 kb on - strand, within RPSI07_RS06890 at 1835.785 kb on - strand, within RPSI07_RS06890 at 1835.785 kb on - strand, within RPSI07_RS06890 at 1835.922 kb on + strand, within RPSI07_RS06890 at 1835.923 kb on - strand, within RPSI07_RS06890 at 1835.923 kb on - strand, within RPSI07_RS06890 at 1835.923 kb on - strand, within RPSI07_RS06890 at 1835.923 kb on - strand, within RPSI07_RS06890 at 1836.026 kb on + strand, within RPSI07_RS06890 at 1836.027 kb on - strand, within RPSI07_RS06890 at 1836.027 kb on - strand, within RPSI07_RS06890 at 1836.027 kb on - strand, within RPSI07_RS06890 at 1836.033 kb on - strand, within RPSI07_RS06890 at 1836.033 kb on - strand, within RPSI07_RS06890 at 1836.054 kb on + strand, within RPSI07_RS06890 at 1836.055 kb on - strand, within RPSI07_RS06890 at 1836.058 kb on + strand, within RPSI07_RS06890 at 1836.058 kb on + strand, within RPSI07_RS06890 at 1836.059 kb on - strand, within RPSI07_RS06890 at 1836.059 kb on - strand, within RPSI07_RS06890 at 1836.065 kb on + strand, within RPSI07_RS06890 at 1836.066 kb on - strand, within RPSI07_RS06890 at 1836.095 kb on + strand, within RPSI07_RS06890 at 1836.221 kb on + strand, within RPSI07_RS06890 at 1836.221 kb on + strand, within RPSI07_RS06890 at 1836.221 kb on + strand, within RPSI07_RS06890 at 1836.222 kb on - strand, within RPSI07_RS06890 at 1836.222 kb on - strand, within RPSI07_RS06890 at 1836.222 kb on - strand, within RPSI07_RS06890 at 1836.222 kb on - strand, within RPSI07_RS06890 at 1836.231 kb on + strand, within RPSI07_RS06890 at 1836.231 kb on + strand, within RPSI07_RS06890 at 1836.231 kb on + strand, within RPSI07_RS06890 at 1836.231 kb on + strand, within RPSI07_RS06890 at 1836.231 kb on + strand, within RPSI07_RS06890 at 1836.231 kb on + strand, within RPSI07_RS06890 at 1836.232 kb on - strand at 1836.232 kb on - strand at 1836.291 kb on + strand at 1836.291 kb on + strand at 1836.329 kb on + strand at 1836.329 kb on + strand at 1836.329 kb on + strand at 1836.329 kb on + strand at 1836.329 kb on + strand at 1836.330 kb on - strand at 1836.347 kb on - strand at 1836.393 kb on + strand at 1836.462 kb on + strand at 1836.462 kb on + strand at 1836.462 kb on + strand at 1836.494 kb on + strand, within RPSI07_RS24675 at 1836.494 kb on + strand, within RPSI07_RS24675 at 1836.495 kb on - strand, within RPSI07_RS24675 at 1836.495 kb on - strand, within RPSI07_RS24675 at 1836.683 kb on + strand, within RPSI07_RS24675 at 1836.683 kb on + strand, within RPSI07_RS24675 at 1836.683 kb on + strand, within RPSI07_RS24675 at 1836.684 kb on - strand, within RPSI07_RS24675 at 1836.684 kb on - strand, within RPSI07_RS24675 at 1836.684 kb on - strand, within RPSI07_RS24675 at 1836.684 kb on - strand, within RPSI07_RS24675 at 1836.684 kb on - strand, within RPSI07_RS24675 at 1836.684 kb on - strand, within RPSI07_RS24675 at 1836.684 kb on - strand, within RPSI07_RS24675 at 1836.684 kb on - strand, within RPSI07_RS24675 at 1836.692 kb on + strand, within RPSI07_RS24675 at 1836.692 kb on + strand, within RPSI07_RS24675 at 1836.692 kb on + strand, within RPSI07_RS24675 at 1836.692 kb on + strand, within RPSI07_RS24675 at 1836.693 kb on - strand, within RPSI07_RS24675 at 1836.793 kb on + strand at 1836.794 kb on - strand at 1836.794 kb on - strand at 1836.876 kb on + strand at 1836.876 kb on + strand at 1836.877 kb on - strand at 1836.877 kb on - strand at 1836.904 kb on + strand at 1836.904 kb on + strand at 1836.904 kb on + strand at 1836.904 kb on + strand at 1836.904 kb on + strand at 1836.905 kb on - strand at 1836.905 kb on - strand at 1836.979 kb on + strand at 1836.979 kb on + strand at 1836.979 kb on + strand at 1836.979 kb on + strand at 1836.979 kb on + strand at 1836.980 kb on - strand at 1836.980 kb on - strand at 1836.980 kb on - strand at 1836.980 kb on - strand at 1836.980 kb on - strand at 1836.980 kb on - strand at 1836.980 kb on - strand at 1836.980 kb on - strand at 1836.996 kb on + strand at 1836.996 kb on + strand at 1836.996 kb on + strand at 1836.996 kb on + strand at 1836.996 kb on + strand at 1836.997 kb on - strand at 1836.997 kb on - strand at 1836.997 kb on - strand at 1836.997 kb on - strand at 1837.044 kb on + strand at 1837.044 kb on + strand at 1837.045 kb on - strand at 1837.045 kb on - strand at 1837.045 kb on - strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.046 kb on + strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.047 kb on - strand at 1837.125 kb on + strand at 1837.126 kb on - strand at 1837.162 kb on + strand at 1837.162 kb on + strand at 1837.162 kb on + strand at 1837.194 kb on + strand at 1837.213 kb on + strand at 1837.213 kb on + strand at 1837.213 kb on + strand at 1837.213 kb on + strand at 1837.214 kb on - strand at 1837.251 kb on + strand at 1837.251 kb on + strand at 1837.251 kb on + strand at 1837.251 kb on + strand at 1837.252 kb on - strand at 1837.252 kb on - strand at 1837.252 kb on - strand at 1837.252 kb on - strand at 1837.281 kb on + strand at 1837.371 kb on - strand at 1837.386 kb on + strand at 1837.421 kb on + strand at 1837.439 kb on + strand at 1837.489 kb on + strand at 1837.489 kb on + strand at 1837.489 kb on + strand at 1837.489 kb on + strand at 1837.489 kb on + strand at 1837.489 kb on + strand at 1837.489 kb on + strand at 1837.489 kb on + strand at 1837.490 kb on - strand at 1837.490 kb on - strand at 1837.490 kb on - strand at 1837.490 kb on - strand at 1837.490 kb on - strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.491 kb on + strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.492 kb on - strand at 1837.506 kb on + strand at 1837.506 kb on + strand at 1837.506 kb on + strand at 1837.506 kb on + strand at 1837.608 kb on + strand at 1837.609 kb on - strand at 1837.609 kb on - strand at 1837.609 kb on - strand at 1837.615 kb on + strand at 1837.615 kb on + strand at 1837.615 kb on + strand at 1837.615 kb on + strand at 1837.615 kb on + strand at 1837.615 kb on + strand at 1837.616 kb on - strand at 1837.616 kb on - strand at 1837.616 kb on - strand at 1837.616 kb on - strand at 1837.657 kb on + strand at 1837.658 kb on - strand at 1837.658 kb on - strand at 1837.713 kb on + strand at 1837.714 kb on - strand at 1837.726 kb on + strand at 1837.734 kb on + strand at 1837.735 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 1,835,473 - RPSI07_RS06885 0.89 -1.8 1,835,473 - RPSI07_RS06885 0.89 -0.8 1,835,473 - RPSI07_RS06885 0.89 +0.7 1,835,664 + +0.0 1,835,666 - +1.0 1,835,705 + -1.2 1,835,706 - +1.0 1,835,706 - +0.4 1,835,709 - +0.5 1,835,709 - -0.6 1,835,784 + RPSI07_RS06890 0.17 +0.4 1,835,784 + RPSI07_RS06890 0.17 -0.8 1,835,784 + RPSI07_RS06890 0.17 -0.2 1,835,785 - RPSI07_RS06890 0.17 +0.3 1,835,785 - RPSI07_RS06890 0.17 -0.5 1,835,785 - RPSI07_RS06890 0.17 -1.5 1,835,785 - RPSI07_RS06890 0.17 -0.1 1,835,785 - RPSI07_RS06890 0.17 -0.7 1,835,922 + RPSI07_RS06890 0.39 +0.8 1,835,923 - RPSI07_RS06890 0.39 -1.9 1,835,923 - RPSI07_RS06890 0.39 -0.7 1,835,923 - RPSI07_RS06890 0.39 -0.5 1,835,923 - RPSI07_RS06890 0.39 -0.1 1,836,026 + RPSI07_RS06890 0.56 +1.0 1,836,027 - RPSI07_RS06890 0.56 -0.6 1,836,027 - RPSI07_RS06890 0.56 -2.5 1,836,027 - RPSI07_RS06890 0.56 -0.6 1,836,033 - RPSI07_RS06890 0.57 -1.1 1,836,033 - RPSI07_RS06890 0.57 -0.3 1,836,054 + RPSI07_RS06890 0.61 -0.9 1,836,055 - RPSI07_RS06890 0.61 -0.3 1,836,058 + RPSI07_RS06890 0.61 -0.2 1,836,058 + RPSI07_RS06890 0.61 -0.9 1,836,059 - RPSI07_RS06890 0.62 -0.0 1,836,059 - RPSI07_RS06890 0.62 -0.4 1,836,065 + RPSI07_RS06890 0.63 +0.8 1,836,066 - RPSI07_RS06890 0.63 +1.6 1,836,095 + RPSI07_RS06890 0.67 -1.6 1,836,221 + RPSI07_RS06890 0.88 +0.4 1,836,221 + RPSI07_RS06890 0.88 +0.4 1,836,221 + RPSI07_RS06890 0.88 -0.9 1,836,222 - RPSI07_RS06890 0.88 +1.0 1,836,222 - RPSI07_RS06890 0.88 -1.6 1,836,222 - RPSI07_RS06890 0.88 -1.3 1,836,222 - RPSI07_RS06890 0.88 -0.8 1,836,231 + RPSI07_RS06890 0.90 -0.2 1,836,231 + RPSI07_RS06890 0.90 -1.8 1,836,231 + RPSI07_RS06890 0.90 -0.4 1,836,231 + RPSI07_RS06890 0.90 -0.1 1,836,231 + RPSI07_RS06890 0.90 +0.6 1,836,231 + RPSI07_RS06890 0.90 +0.4 1,836,232 - +0.5 1,836,232 - +0.9 1,836,291 + -0.5 1,836,291 + +1.9 1,836,329 + -0.3 1,836,329 + -1.5 1,836,329 + +0.1 1,836,329 + +0.0 1,836,329 + -0.6 1,836,330 - +0.4 1,836,347 - -0.4 1,836,393 + +1.0 1,836,462 + +0.2 1,836,462 + -0.4 1,836,462 + -0.5 1,836,494 + RPSI07_RS24675 0.11 +0.5 1,836,494 + RPSI07_RS24675 0.11 +0.5 1,836,495 - RPSI07_RS24675 0.11 +0.6 1,836,495 - RPSI07_RS24675 0.11 +0.0 1,836,683 + RPSI07_RS24675 0.67 +1.0 1,836,683 + RPSI07_RS24675 0.67 +0.3 1,836,683 + RPSI07_RS24675 0.67 +0.0 1,836,684 - RPSI07_RS24675 0.67 -0.6 1,836,684 - RPSI07_RS24675 0.67 -0.4 1,836,684 - RPSI07_RS24675 0.67 -1.0 1,836,684 - RPSI07_RS24675 0.67 +1.2 1,836,684 - RPSI07_RS24675 0.67 +0.6 1,836,684 - RPSI07_RS24675 0.67 +1.5 1,836,684 - RPSI07_RS24675 0.67 -1.8 1,836,684 - RPSI07_RS24675 0.67 +0.1 1,836,692 + RPSI07_RS24675 0.69 +0.3 1,836,692 + RPSI07_RS24675 0.69 +0.5 1,836,692 + RPSI07_RS24675 0.69 -1.1 1,836,692 + RPSI07_RS24675 0.69 +1.0 1,836,693 - RPSI07_RS24675 0.70 +0.0 1,836,793 + +0.3 1,836,794 - +0.2 1,836,794 - -0.3 1,836,876 + +0.0 1,836,876 + -0.1 1,836,877 - -1.3 1,836,877 - +0.0 1,836,904 + -0.6 1,836,904 + -0.5 1,836,904 + -1.5 1,836,904 + +1.3 1,836,904 + +0.9 1,836,905 - +1.9 1,836,905 - +0.2 1,836,979 + -2.8 1,836,979 + -0.0 1,836,979 + -0.4 1,836,979 + -1.5 1,836,979 + +1.8 1,836,980 - -0.2 1,836,980 - +0.2 1,836,980 - +0.2 1,836,980 - -0.8 1,836,980 - +1.2 1,836,980 - +0.5 1,836,980 - +0.6 1,836,980 - +1.5 1,836,996 + +1.0 1,836,996 + -0.4 1,836,996 + -2.3 1,836,996 + +0.5 1,836,996 + -0.2 1,836,997 - -0.7 1,836,997 - -0.5 1,836,997 - +1.0 1,836,997 - -1.6 1,837,044 + -0.3 1,837,044 + -0.3 1,837,045 - +0.8 1,837,045 - -1.3 1,837,045 - -1.2 1,837,046 + +0.1 1,837,046 + -0.6 1,837,046 + +0.1 1,837,046 + -0.6 1,837,046 + -1.2 1,837,046 + -0.8 1,837,046 + -0.1 1,837,046 + -0.6 1,837,046 + +0.9 1,837,046 + +1.4 1,837,046 + -1.0 1,837,046 + +2.1 1,837,047 - +0.5 1,837,047 - -0.9 1,837,047 - -0.3 1,837,047 - -0.6 1,837,047 - +0.5 1,837,047 - -2.8 1,837,047 - +0.2 1,837,047 - -0.4 1,837,047 - -0.4 1,837,047 - +1.1 1,837,047 - +0.3 1,837,047 - -1.0 1,837,047 - +0.9 1,837,047 - -0.3 1,837,125 + -0.5 1,837,126 - +0.1 1,837,162 + -1.5 1,837,162 + -0.9 1,837,162 + -0.1 1,837,194 + -0.5 1,837,213 + +1.0 1,837,213 + +1.0 1,837,213 + -2.3 1,837,213 + +0.9 1,837,214 - -0.6 1,837,251 + +0.6 1,837,251 + -1.3 1,837,251 + +1.0 1,837,251 + +1.7 1,837,252 - -0.1 1,837,252 - +1.0 1,837,252 - -1.3 1,837,252 - +3.5 1,837,281 + -1.4 1,837,371 - +0.8 1,837,386 + -1.2 1,837,421 + -0.3 1,837,439 + -0.6 1,837,489 + -0.6 1,837,489 + +0.2 1,837,489 + -0.5 1,837,489 + -0.5 1,837,489 + +0.4 1,837,489 + -0.0 1,837,489 + +0.2 1,837,489 + -1.8 1,837,490 - +1.3 1,837,490 - +0.7 1,837,490 - -0.9 1,837,490 - -1.8 1,837,490 - +3.0 1,837,491 + -0.2 1,837,491 + -1.0 1,837,491 + +3.5 1,837,491 + -0.0 1,837,491 + -0.3 1,837,491 + -0.0 1,837,491 + +0.2 1,837,491 + +0.0 1,837,491 + -2.0 1,837,491 + +0.2 1,837,491 + -0.3 1,837,491 + -0.2 1,837,492 - +0.6 1,837,492 - -2.2 1,837,492 - +0.8 1,837,492 - -0.5 1,837,492 - +0.3 1,837,492 - +0.7 1,837,492 - +0.8 1,837,492 - -0.5 1,837,492 - +2.0 1,837,492 - +0.4 1,837,492 - +0.6 1,837,492 - +0.0 1,837,492 - +0.9 1,837,492 - -0.6 1,837,506 + -0.2 1,837,506 + +0.1 1,837,506 + -0.3 1,837,506 + +0.2 1,837,608 + +2.6 1,837,609 - -1.0 1,837,609 - +0.0 1,837,609 - -1.8 1,837,615 + -0.7 1,837,615 + -1.0 1,837,615 + +1.6 1,837,615 + +0.8 1,837,615 + +0.1 1,837,615 + +0.3 1,837,616 - -0.1 1,837,616 - +2.1 1,837,616 - +1.1 1,837,616 - +0.8 1,837,657 + -0.6 1,837,658 - -0.5 1,837,658 - -2.7 1,837,713 + +0.3 1,837,714 - -0.1 1,837,726 + -0.3 1,837,734 + -0.4 1,837,735 - -1.4
Or see this region's nucleotide sequence