Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS17940 and RPSI07_RS17945 are separated by 12 nucleotides RPSI07_RS17945 and RPSI07_RS17950 overlap by 4 nucleotides RPSI07_RS17950 and RPSI07_RS17955 overlap by 4 nucleotides
RPSI07_RS17940: RPSI07_RS17940 - putative addiction module antidote protein, at 2,231,344 to 2,231,631
_RS17940
RPSI07_RS17945: RPSI07_RS17945 - hypothetical protein, at 2,231,644 to 2,232,489
_RS17945
RPSI07_RS17950: RPSI07_RS17950 - hypothetical protein, at 2,232,486 to 2,233,523
_RS17950
RPSI07_RS17955: RPSI07_RS17955 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 2,233,520 to 2,234,809
_RS17955
Position (kb)
2232
2233
2234 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2231.533 kb on + strand, within RPSI07_RS17940 at 2231.533 kb on + strand, within RPSI07_RS17940 at 2231.645 kb on + strand at 2231.765 kb on + strand, within RPSI07_RS17945 at 2232.206 kb on + strand, within RPSI07_RS17945 at 2232.601 kb on + strand, within RPSI07_RS17950 at 2232.618 kb on + strand, within RPSI07_RS17950 at 2232.619 kb on + strand, within RPSI07_RS17950 at 2232.619 kb on + strand, within RPSI07_RS17950 at 2232.620 kb on - strand, within RPSI07_RS17950 at 2232.620 kb on - strand, within RPSI07_RS17950 at 2232.675 kb on - strand, within RPSI07_RS17950 at 2232.748 kb on + strand, within RPSI07_RS17950 at 2232.748 kb on + strand, within RPSI07_RS17950 at 2232.748 kb on + strand, within RPSI07_RS17950 at 2232.749 kb on - strand, within RPSI07_RS17950 at 2232.749 kb on - strand, within RPSI07_RS17950 at 2232.778 kb on + strand, within RPSI07_RS17950 at 2232.998 kb on - strand, within RPSI07_RS17950 at 2233.169 kb on - strand, within RPSI07_RS17950 at 2233.406 kb on - strand, within RPSI07_RS17950 at 2233.804 kb on + strand, within RPSI07_RS17955 at 2233.804 kb on + strand, within RPSI07_RS17955 at 2233.804 kb on + strand, within RPSI07_RS17955 at 2233.804 kb on + strand, within RPSI07_RS17955 at 2233.805 kb on - strand, within RPSI07_RS17955 at 2233.805 kb on - strand, within RPSI07_RS17955 at 2233.812 kb on + strand, within RPSI07_RS17955 at 2233.974 kb on + strand, within RPSI07_RS17955 at 2233.974 kb on + strand, within RPSI07_RS17955 at 2233.974 kb on + strand, within RPSI07_RS17955 at 2233.974 kb on + strand, within RPSI07_RS17955 at 2233.975 kb on - strand, within RPSI07_RS17955 at 2234.357 kb on + strand at 2234.358 kb on - strand, within RPSI07_RS17955 at 2234.358 kb on - strand, within RPSI07_RS17955 at 2234.496 kb on + strand, within RPSI07_RS17955
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 2,231,533 + RPSI07_RS17940 0.66 +0.3 2,231,533 + RPSI07_RS17940 0.66 +0.5 2,231,645 + -0.1 2,231,765 + RPSI07_RS17945 0.14 -0.9 2,232,206 + RPSI07_RS17945 0.66 +0.7 2,232,601 + RPSI07_RS17950 0.11 -0.7 2,232,618 + RPSI07_RS17950 0.13 -0.6 2,232,619 + RPSI07_RS17950 0.13 +0.8 2,232,619 + RPSI07_RS17950 0.13 -0.7 2,232,620 - RPSI07_RS17950 0.13 +0.1 2,232,620 - RPSI07_RS17950 0.13 -0.1 2,232,675 - RPSI07_RS17950 0.18 +1.0 2,232,748 + RPSI07_RS17950 0.25 -0.7 2,232,748 + RPSI07_RS17950 0.25 -2.7 2,232,748 + RPSI07_RS17950 0.25 -1.4 2,232,749 - RPSI07_RS17950 0.25 -0.2 2,232,749 - RPSI07_RS17950 0.25 -0.3 2,232,778 + RPSI07_RS17950 0.28 -1.5 2,232,998 - RPSI07_RS17950 0.49 -0.6 2,233,169 - RPSI07_RS17950 0.66 +1.2 2,233,406 - RPSI07_RS17950 0.89 -1.8 2,233,804 + RPSI07_RS17955 0.22 +0.1 2,233,804 + RPSI07_RS17955 0.22 +0.7 2,233,804 + RPSI07_RS17955 0.22 +0.6 2,233,804 + RPSI07_RS17955 0.22 -0.2 2,233,805 - RPSI07_RS17955 0.22 +0.2 2,233,805 - RPSI07_RS17955 0.22 -0.2 2,233,812 + RPSI07_RS17955 0.23 -0.4 2,233,974 + RPSI07_RS17955 0.35 -1.2 2,233,974 + RPSI07_RS17955 0.35 +2.1 2,233,974 + RPSI07_RS17955 0.35 +0.6 2,233,974 + RPSI07_RS17955 0.35 -0.5 2,233,975 - RPSI07_RS17955 0.35 +0.3 2,234,357 + +1.1 2,234,358 - RPSI07_RS17955 0.65 -0.4 2,234,358 - RPSI07_RS17955 0.65 -0.9 2,234,496 + RPSI07_RS17955 0.76 -0.1
Or see this region's nucleotide sequence