Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16560 and RPSI07_RS16565 are separated by 495 nucleotides RPSI07_RS16565 and RPSI07_RS16570 are separated by 341 nucleotides
RPSI07_RS16560: RPSI07_RS16560 - MFS transporter, at 1,876,300 to 1,877,739
_RS16560
RPSI07_RS16565: RPSI07_RS16565 - hypothetical protein, at 1,878,235 to 1,878,597
_RS16565
RPSI07_RS16570: RPSI07_RS16570 - Type III effector protein (Skwp 4), at 1,878,939 to 1,888,208
_RS16570
Position (kb)
1878
1879 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1877.255 kb on + strand, within RPSI07_RS16560 at 1877.255 kb on + strand, within RPSI07_RS16560 at 1877.255 kb on + strand, within RPSI07_RS16560 at 1877.255 kb on + strand, within RPSI07_RS16560 at 1877.255 kb on + strand, within RPSI07_RS16560 at 1877.255 kb on + strand, within RPSI07_RS16560 at 1877.255 kb on + strand, within RPSI07_RS16560 at 1877.255 kb on + strand, within RPSI07_RS16560 at 1877.256 kb on - strand, within RPSI07_RS16560 at 1877.256 kb on - strand, within RPSI07_RS16560 at 1877.256 kb on - strand, within RPSI07_RS16560 at 1877.256 kb on - strand, within RPSI07_RS16560 at 1877.256 kb on - strand, within RPSI07_RS16560 at 1877.256 kb on - strand, within RPSI07_RS16560 at 1877.366 kb on + strand, within RPSI07_RS16560 at 1877.366 kb on + strand, within RPSI07_RS16560 at 1877.406 kb on - strand, within RPSI07_RS16560 at 1877.406 kb on - strand, within RPSI07_RS16560 at 1877.406 kb on - strand, within RPSI07_RS16560 at 1877.552 kb on + strand, within RPSI07_RS16560 at 1877.552 kb on + strand, within RPSI07_RS16560 at 1877.573 kb on + strand, within RPSI07_RS16560 at 1877.574 kb on - strand, within RPSI07_RS16560 at 1877.574 kb on - strand, within RPSI07_RS16560 at 1877.593 kb on + strand, within RPSI07_RS16560 at 1877.594 kb on - strand, within RPSI07_RS16560 at 1877.632 kb on - strand at 1877.782 kb on + strand at 1877.782 kb on + strand at 1877.782 kb on + strand at 1877.782 kb on + strand at 1877.783 kb on - strand at 1877.783 kb on - strand at 1877.863 kb on - strand at 1878.076 kb on + strand at 1878.167 kb on + strand at 1878.167 kb on + strand at 1878.230 kb on + strand at 1878.337 kb on - strand, within RPSI07_RS16565 at 1878.738 kb on + strand at 1878.738 kb on + strand at 1878.739 kb on - strand at 1878.759 kb on + strand at 1878.759 kb on + strand at 1878.760 kb on - strand at 1878.760 kb on - strand at 1878.760 kb on - strand at 1878.760 kb on - strand at 1878.760 kb on - strand at 1878.763 kb on + strand at 1878.764 kb on - strand at 1878.764 kb on - strand at 1878.764 kb on - strand at 1878.764 kb on - strand at 1878.764 kb on - strand at 1878.780 kb on - strand at 1878.780 kb on - strand at 1878.780 kb on - strand at 1878.783 kb on + strand at 1878.783 kb on + strand at 1878.784 kb on - strand at 1878.784 kb on - strand at 1878.849 kb on + strand at 1878.849 kb on + strand at 1878.867 kb on - strand at 1879.007 kb on + strand at 1879.008 kb on - strand at 1879.008 kb on - strand at 1879.185 kb on + strand at 1879.185 kb on + strand at 1879.185 kb on + strand at 1879.186 kb on - strand at 1879.186 kb on - strand at 1879.186 kb on - strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.202 kb on - strand at 1879.202 kb on - strand at 1879.202 kb on - strand at 1879.202 kb on - strand at 1879.408 kb on + strand at 1879.408 kb on + strand at 1879.408 kb on + strand at 1879.409 kb on - strand at 1879.409 kb on - strand at 1879.410 kb on - strand at 1879.553 kb on + strand at 1879.553 kb on + strand at 1879.553 kb on + strand at 1879.553 kb on + strand at 1879.554 kb on - strand at 1879.554 kb on - strand at 1879.554 kb on - strand at 1879.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 1,877,255 + RPSI07_RS16560 0.66 -0.3 1,877,255 + RPSI07_RS16560 0.66 -0.8 1,877,255 + RPSI07_RS16560 0.66 -0.3 1,877,255 + RPSI07_RS16560 0.66 +1.9 1,877,255 + RPSI07_RS16560 0.66 -0.5 1,877,255 + RPSI07_RS16560 0.66 +0.0 1,877,255 + RPSI07_RS16560 0.66 +1.0 1,877,255 + RPSI07_RS16560 0.66 -0.8 1,877,256 - RPSI07_RS16560 0.66 -0.6 1,877,256 - RPSI07_RS16560 0.66 -0.6 1,877,256 - RPSI07_RS16560 0.66 +1.9 1,877,256 - RPSI07_RS16560 0.66 +0.4 1,877,256 - RPSI07_RS16560 0.66 +1.0 1,877,256 - RPSI07_RS16560 0.66 +0.3 1,877,366 + RPSI07_RS16560 0.74 +0.3 1,877,366 + RPSI07_RS16560 0.74 +0.6 1,877,406 - RPSI07_RS16560 0.77 +0.0 1,877,406 - RPSI07_RS16560 0.77 +1.2 1,877,406 - RPSI07_RS16560 0.77 +0.0 1,877,552 + RPSI07_RS16560 0.87 -0.6 1,877,552 + RPSI07_RS16560 0.87 -0.8 1,877,573 + RPSI07_RS16560 0.88 +4.6 1,877,574 - RPSI07_RS16560 0.88 -2.1 1,877,574 - RPSI07_RS16560 0.88 -0.6 1,877,593 + RPSI07_RS16560 0.90 +0.4 1,877,594 - RPSI07_RS16560 0.90 -0.6 1,877,632 - -0.9 1,877,782 + -0.6 1,877,782 + +0.3 1,877,782 + +0.8 1,877,782 + +0.3 1,877,783 - +0.3 1,877,783 - -1.2 1,877,863 - -1.0 1,878,076 + +0.4 1,878,167 + -2.3 1,878,167 + +0.6 1,878,230 + -1.3 1,878,337 - RPSI07_RS16565 0.28 +0.5 1,878,738 + -1.0 1,878,738 + +0.3 1,878,739 - -0.0 1,878,759 + +2.0 1,878,759 + -2.0 1,878,760 - +1.4 1,878,760 - -3.1 1,878,760 - -0.8 1,878,760 - +0.0 1,878,760 - -0.1 1,878,763 + -1.7 1,878,764 - -0.4 1,878,764 - -0.6 1,878,764 - -0.3 1,878,764 - +0.5 1,878,764 - +0.9 1,878,780 - +2.4 1,878,780 - -1.6 1,878,780 - +0.8 1,878,783 + +0.7 1,878,783 + -1.3 1,878,784 - +0.0 1,878,784 - -0.2 1,878,849 + -1.4 1,878,849 + +2.6 1,878,867 - +0.0 1,879,007 + +0.4 1,879,008 - +0.3 1,879,008 - +2.0 1,879,185 + -0.3 1,879,185 + +0.4 1,879,185 + -1.6 1,879,186 - -0.6 1,879,186 - +1.0 1,879,186 - +0.6 1,879,201 + -2.0 1,879,201 + +1.6 1,879,201 + -0.7 1,879,201 + +0.7 1,879,201 + +1.4 1,879,201 + -2.0 1,879,201 + -1.6 1,879,201 + -0.8 1,879,202 - -1.1 1,879,202 - +0.0 1,879,202 - +0.3 1,879,202 - -0.2 1,879,408 + +0.7 1,879,408 + -2.3 1,879,408 + -0.2 1,879,409 - +1.0 1,879,409 - +0.9 1,879,410 - -1.0 1,879,553 + -0.7 1,879,553 + +0.1 1,879,553 + -0.2 1,879,553 + -0.6 1,879,554 - -0.8 1,879,554 - +0.6 1,879,554 - -0.6 1,879,554 - +1.0
Or see this region's nucleotide sequence