Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS06990 and RPSI07_RS06995 are separated by 208 nucleotides RPSI07_RS06995 and RPSI07_RS07000 are separated by 127 nucleotides RPSI07_RS07000 and RPSI07_RS07005 are separated by 100 nucleotides RPSI07_RS07005 and RPSI07_RS24700 are separated by 267 nucleotides RPSI07_RS24700 and RPSI07_RS07015 are separated by 165 nucleotides
RPSI07_RS06990: RPSI07_RS06990 - YopT-type cysteine protease domain-containing protein, at 1,859,342 to 1,860,304
_RS06990
RPSI07_RS06995: RPSI07_RS06995 - hypothetical protein, at 1,860,513 to 1,860,981
_RS06995
RPSI07_RS07000: RPSI07_RS07000 - hypothetical protein, at 1,861,109 to 1,861,465
_RS07000
RPSI07_RS07005: RPSI07_RS07005 - hypothetical protein, at 1,861,566 to 1,861,754
_RS07005
RPSI07_RS24700: RPSI07_RS24700 - hypothetical protein, at 1,862,022 to 1,862,255
_RS24700
RPSI07_RS07015: RPSI07_RS07015 - hypothetical protein, at 1,862,421 to 1,862,786
_RS07015
Position (kb)
1861
1862 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1860.335 kb on + strand at 1860.335 kb on + strand at 1860.335 kb on + strand at 1860.335 kb on + strand at 1860.335 kb on + strand at 1860.336 kb on - strand at 1860.358 kb on - strand at 1860.405 kb on + strand at 1860.406 kb on - strand at 1860.443 kb on + strand at 1860.444 kb on - strand at 1860.606 kb on + strand, within RPSI07_RS06995 at 1860.700 kb on + strand, within RPSI07_RS06995 at 1860.700 kb on + strand, within RPSI07_RS06995 at 1860.700 kb on + strand, within RPSI07_RS06995 at 1860.700 kb on + strand, within RPSI07_RS06995 at 1860.701 kb on - strand, within RPSI07_RS06995 at 1860.701 kb on - strand, within RPSI07_RS06995 at 1860.701 kb on - strand, within RPSI07_RS06995 at 1860.701 kb on - strand, within RPSI07_RS06995 at 1860.943 kb on + strand at 1860.944 kb on - strand at 1860.944 kb on - strand at 1860.944 kb on - strand at 1861.002 kb on + strand at 1861.242 kb on - strand, within RPSI07_RS07000 at 1861.242 kb on - strand, within RPSI07_RS07000 at 1861.242 kb on - strand, within RPSI07_RS07000 at 1861.242 kb on - strand, within RPSI07_RS07000 at 1861.242 kb on - strand, within RPSI07_RS07000 at 1861.242 kb on - strand, within RPSI07_RS07000 at 1861.253 kb on + strand, within RPSI07_RS07000 at 1861.253 kb on + strand, within RPSI07_RS07000 at 1861.253 kb on + strand, within RPSI07_RS07000 at 1861.253 kb on + strand, within RPSI07_RS07000 at 1861.370 kb on + strand, within RPSI07_RS07000 at 1861.370 kb on + strand, within RPSI07_RS07000 at 1861.370 kb on + strand, within RPSI07_RS07000 at 1861.370 kb on + strand, within RPSI07_RS07000 at 1861.370 kb on + strand, within RPSI07_RS07000 at 1861.371 kb on - strand, within RPSI07_RS07000 at 1861.411 kb on + strand, within RPSI07_RS07000 at 1861.412 kb on + strand, within RPSI07_RS07000 at 1861.412 kb on + strand, within RPSI07_RS07000 at 1861.412 kb on + strand, within RPSI07_RS07000 at 1861.412 kb on + strand, within RPSI07_RS07000 at 1861.412 kb on + strand, within RPSI07_RS07000 at 1861.412 kb on + strand, within RPSI07_RS07000 at 1861.412 kb on + strand, within RPSI07_RS07000 at 1861.412 kb on + strand, within RPSI07_RS07000 at 1861.412 kb on + strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.413 kb on - strand, within RPSI07_RS07000 at 1861.415 kb on - strand, within RPSI07_RS07000 at 1861.437 kb on - strand at 1861.473 kb on + strand at 1861.473 kb on + strand at 1861.474 kb on - strand at 1861.527 kb on + strand at 1861.527 kb on + strand at 1861.527 kb on + strand at 1861.528 kb on - strand at 1861.528 kb on - strand at 1861.528 kb on - strand at 1861.528 kb on - strand at 1861.528 kb on - strand at 1861.528 kb on - strand at 1861.528 kb on - strand at 1861.528 kb on - strand at 1861.528 kb on - strand at 1861.588 kb on - strand, within RPSI07_RS07005 at 1861.588 kb on - strand, within RPSI07_RS07005 at 1861.588 kb on - strand, within RPSI07_RS07005 at 1861.588 kb on - strand, within RPSI07_RS07005 at 1861.588 kb on - strand, within RPSI07_RS07005 at 1861.588 kb on - strand, within RPSI07_RS07005 at 1861.588 kb on - strand, within RPSI07_RS07005 at 1861.588 kb on - strand, within RPSI07_RS07005 at 1861.701 kb on + strand, within RPSI07_RS07005 at 1861.701 kb on + strand, within RPSI07_RS07005 at 1861.701 kb on + strand, within RPSI07_RS07005 at 1861.702 kb on - strand, within RPSI07_RS07005 at 1861.702 kb on - strand, within RPSI07_RS07005 at 1861.702 kb on - strand, within RPSI07_RS07005 at 1861.712 kb on + strand, within RPSI07_RS07005 at 1861.731 kb on + strand, within RPSI07_RS07005 at 1861.731 kb on + strand, within RPSI07_RS07005 at 1861.731 kb on + strand, within RPSI07_RS07005 at 1861.731 kb on + strand, within RPSI07_RS07005 at 1861.732 kb on - strand, within RPSI07_RS07005 at 1861.828 kb on + strand at 1861.828 kb on + strand at 1861.829 kb on - strand at 1861.829 kb on - strand at 1861.829 kb on - strand at 1861.832 kb on + strand at 1861.832 kb on + strand at 1861.832 kb on + strand at 1861.833 kb on - strand at 1861.833 kb on - strand at 1861.833 kb on - strand at 1861.833 kb on - strand at 1861.833 kb on - strand at 1861.833 kb on - strand at 1861.889 kb on + strand at 1861.890 kb on - strand at 1861.890 kb on - strand at 1861.890 kb on - strand at 1861.890 kb on - strand at 1861.890 kb on - strand at 1861.890 kb on - strand at 1861.949 kb on + strand at 1861.949 kb on + strand at 1861.950 kb on - strand at 1861.950 kb on - strand at 1861.950 kb on - strand at 1861.950 kb on - strand at 1861.951 kb on - strand at 1862.099 kb on + strand, within RPSI07_RS24700 at 1862.099 kb on + strand, within RPSI07_RS24700 at 1862.100 kb on - strand, within RPSI07_RS24700 at 1862.185 kb on + strand, within RPSI07_RS24700 at 1862.185 kb on + strand, within RPSI07_RS24700 at 1862.186 kb on - strand, within RPSI07_RS24700 at 1862.187 kb on - strand, within RPSI07_RS24700 at 1862.203 kb on + strand, within RPSI07_RS24700 at 1862.203 kb on + strand, within RPSI07_RS24700 at 1862.203 kb on + strand, within RPSI07_RS24700 at 1862.204 kb on - strand, within RPSI07_RS24700 at 1862.204 kb on - strand, within RPSI07_RS24700 at 1862.204 kb on - strand, within RPSI07_RS24700 at 1862.219 kb on + strand, within RPSI07_RS24700 at 1862.219 kb on + strand, within RPSI07_RS24700 at 1862.220 kb on - strand, within RPSI07_RS24700 at 1862.220 kb on - strand, within RPSI07_RS24700 at 1862.220 kb on - strand, within RPSI07_RS24700 at 1862.220 kb on - strand, within RPSI07_RS24700 at 1862.242 kb on + strand at 1862.242 kb on + strand at 1862.242 kb on + strand at 1862.242 kb on + strand at 1862.242 kb on + strand at 1862.242 kb on + strand at 1862.242 kb on + strand at 1862.242 kb on + strand at 1862.243 kb on - strand at 1862.243 kb on - strand at 1862.243 kb on - strand at 1862.243 kb on - strand at 1862.243 kb on - strand at 1862.243 kb on - strand at 1862.243 kb on - strand at 1862.293 kb on - strand at 1862.440 kb on + strand at 1862.441 kb on - strand at 1862.441 kb on - strand at 1862.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 1,860,335 + -0.8 1,860,335 + +0.5 1,860,335 + -1.4 1,860,335 + -0.6 1,860,335 + -2.7 1,860,336 - -2.0 1,860,358 - -0.1 1,860,405 + -0.8 1,860,406 - +2.3 1,860,443 + +1.0 1,860,444 - -0.9 1,860,606 + RPSI07_RS06995 0.20 +0.3 1,860,700 + RPSI07_RS06995 0.40 -1.1 1,860,700 + RPSI07_RS06995 0.40 -0.9 1,860,700 + RPSI07_RS06995 0.40 -0.2 1,860,700 + RPSI07_RS06995 0.40 +0.7 1,860,701 - RPSI07_RS06995 0.40 +1.0 1,860,701 - RPSI07_RS06995 0.40 +1.2 1,860,701 - RPSI07_RS06995 0.40 -1.6 1,860,701 - RPSI07_RS06995 0.40 +0.2 1,860,943 + -0.3 1,860,944 - -2.3 1,860,944 - +0.0 1,860,944 - +0.4 1,861,002 + -2.0 1,861,242 - RPSI07_RS07000 0.37 +0.0 1,861,242 - RPSI07_RS07000 0.37 -1.3 1,861,242 - RPSI07_RS07000 0.37 -0.6 1,861,242 - RPSI07_RS07000 0.37 +1.4 1,861,242 - RPSI07_RS07000 0.37 -1.8 1,861,242 - RPSI07_RS07000 0.37 +0.0 1,861,253 + RPSI07_RS07000 0.40 -0.2 1,861,253 + RPSI07_RS07000 0.40 -2.2 1,861,253 + RPSI07_RS07000 0.40 -0.8 1,861,253 + RPSI07_RS07000 0.40 -1.0 1,861,370 + RPSI07_RS07000 0.73 +0.5 1,861,370 + RPSI07_RS07000 0.73 +1.2 1,861,370 + RPSI07_RS07000 0.73 -2.0 1,861,370 + RPSI07_RS07000 0.73 +0.1 1,861,370 + RPSI07_RS07000 0.73 -0.7 1,861,371 - RPSI07_RS07000 0.73 +0.1 1,861,411 + RPSI07_RS07000 0.85 +1.8 1,861,412 + RPSI07_RS07000 0.85 +1.9 1,861,412 + RPSI07_RS07000 0.85 +1.2 1,861,412 + RPSI07_RS07000 0.85 -2.0 1,861,412 + RPSI07_RS07000 0.85 +0.1 1,861,412 + RPSI07_RS07000 0.85 -0.7 1,861,412 + RPSI07_RS07000 0.85 -1.6 1,861,412 + RPSI07_RS07000 0.85 +0.4 1,861,412 + RPSI07_RS07000 0.85 -0.6 1,861,412 + RPSI07_RS07000 0.85 -0.2 1,861,413 - RPSI07_RS07000 0.85 -0.7 1,861,413 - RPSI07_RS07000 0.85 -0.6 1,861,413 - RPSI07_RS07000 0.85 +0.2 1,861,413 - RPSI07_RS07000 0.85 +1.3 1,861,413 - RPSI07_RS07000 0.85 -2.2 1,861,413 - RPSI07_RS07000 0.85 -1.4 1,861,413 - RPSI07_RS07000 0.85 -0.4 1,861,413 - RPSI07_RS07000 0.85 +0.7 1,861,413 - RPSI07_RS07000 0.85 -0.6 1,861,413 - RPSI07_RS07000 0.85 -0.2 1,861,413 - RPSI07_RS07000 0.85 +1.0 1,861,413 - RPSI07_RS07000 0.85 -0.7 1,861,413 - RPSI07_RS07000 0.85 -0.9 1,861,413 - RPSI07_RS07000 0.85 -0.3 1,861,415 - RPSI07_RS07000 0.86 +1.3 1,861,437 - -1.4 1,861,473 + -1.6 1,861,473 + +0.0 1,861,474 - -0.2 1,861,527 + +0.8 1,861,527 + -1.5 1,861,527 + -0.5 1,861,528 - +0.0 1,861,528 - +1.5 1,861,528 - -2.4 1,861,528 - -1.6 1,861,528 - -0.3 1,861,528 - -1.0 1,861,528 - -1.0 1,861,528 - -1.6 1,861,528 - -1.6 1,861,588 - RPSI07_RS07005 0.12 -1.4 1,861,588 - RPSI07_RS07005 0.12 -0.7 1,861,588 - RPSI07_RS07005 0.12 -0.7 1,861,588 - RPSI07_RS07005 0.12 -0.3 1,861,588 - RPSI07_RS07005 0.12 -0.2 1,861,588 - RPSI07_RS07005 0.12 -2.1 1,861,588 - RPSI07_RS07005 0.12 +2.0 1,861,588 - RPSI07_RS07005 0.12 +1.8 1,861,701 + RPSI07_RS07005 0.71 -0.2 1,861,701 + RPSI07_RS07005 0.71 +0.4 1,861,701 + RPSI07_RS07005 0.71 -0.6 1,861,702 - RPSI07_RS07005 0.72 -0.6 1,861,702 - RPSI07_RS07005 0.72 +0.4 1,861,702 - RPSI07_RS07005 0.72 -0.2 1,861,712 + RPSI07_RS07005 0.77 -1.0 1,861,731 + RPSI07_RS07005 0.87 +0.4 1,861,731 + RPSI07_RS07005 0.87 +0.4 1,861,731 + RPSI07_RS07005 0.87 -0.2 1,861,731 + RPSI07_RS07005 0.87 -0.2 1,861,732 - RPSI07_RS07005 0.88 -0.4 1,861,828 + -1.1 1,861,828 + +0.0 1,861,829 - -2.0 1,861,829 - +0.0 1,861,829 - +0.8 1,861,832 + +1.4 1,861,832 + -1.4 1,861,832 + -1.8 1,861,833 - +0.1 1,861,833 - +2.0 1,861,833 - +0.2 1,861,833 - +2.6 1,861,833 - -0.8 1,861,833 - +0.2 1,861,889 + -1.2 1,861,890 - +0.5 1,861,890 - +0.3 1,861,890 - -0.2 1,861,890 - +0.1 1,861,890 - +0.1 1,861,890 - -0.7 1,861,949 + -0.4 1,861,949 + +1.6 1,861,950 - +1.5 1,861,950 - +0.6 1,861,950 - -0.3 1,861,950 - +0.0 1,861,951 - +1.2 1,862,099 + RPSI07_RS24700 0.33 +0.2 1,862,099 + RPSI07_RS24700 0.33 -0.1 1,862,100 - RPSI07_RS24700 0.33 -0.2 1,862,185 + RPSI07_RS24700 0.70 +1.6 1,862,185 + RPSI07_RS24700 0.70 -0.4 1,862,186 - RPSI07_RS24700 0.70 +0.1 1,862,187 - RPSI07_RS24700 0.71 -0.3 1,862,203 + RPSI07_RS24700 0.77 +0.3 1,862,203 + RPSI07_RS24700 0.77 +0.4 1,862,203 + RPSI07_RS24700 0.77 +0.1 1,862,204 - RPSI07_RS24700 0.78 -1.3 1,862,204 - RPSI07_RS24700 0.78 -0.5 1,862,204 - RPSI07_RS24700 0.78 +0.2 1,862,219 + RPSI07_RS24700 0.84 -0.5 1,862,219 + RPSI07_RS24700 0.84 -1.1 1,862,220 - RPSI07_RS24700 0.85 +0.6 1,862,220 - RPSI07_RS24700 0.85 +1.2 1,862,220 - RPSI07_RS24700 0.85 +1.0 1,862,220 - RPSI07_RS24700 0.85 +0.1 1,862,242 + -0.6 1,862,242 + +1.0 1,862,242 + -0.5 1,862,242 + +0.0 1,862,242 + -0.3 1,862,242 + +1.0 1,862,242 + -1.5 1,862,242 + -0.5 1,862,243 - +0.4 1,862,243 - +1.8 1,862,243 - -0.2 1,862,243 - -0.8 1,862,243 - +0.0 1,862,243 - -0.2 1,862,243 - +0.4 1,862,293 - -1.6 1,862,440 + +0.8 1,862,441 - -0.4 1,862,441 - +1.3 1,862,441 - +0.1
Or see this region's nucleotide sequence