Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS01410 and RPSI07_RS01415 are separated by 40 nucleotides RPSI07_RS01415 and RPSI07_RS01420 are separated by 32 nucleotides RPSI07_RS01420 and RPSI07_RS01425 are separated by 22 nucleotides
RPSI07_RS01410: RPSI07_RS01410 - flagellar basal-body rod protein FlgF, at 383,019 to 383,756
_RS01410
RPSI07_RS01415: RPSI07_RS01415 - flagellar basal-body rod protein FlgG, at 383,797 to 384,579
_RS01415
RPSI07_RS01420: RPSI07_RS01420 - flagellar L-ring protein FlgH, at 384,612 to 385,346
_RS01420
RPSI07_RS01425: RPSI07_RS01425 - flagellar basal body P-ring protein FlgI, at 385,369 to 386,478
_RS01425
Position (kb) 384
385
386 Strain fitness (log2 ratio) -2
-1
0
1
2 at 383.932 kb on + strand, within RPSI07_RS01415 at 383.933 kb on - strand, within RPSI07_RS01415 at 384.136 kb on + strand, within RPSI07_RS01415 at 384.448 kb on + strand, within RPSI07_RS01415 at 384.448 kb on + strand, within RPSI07_RS01415 at 384.448 kb on + strand, within RPSI07_RS01415 at 384.448 kb on + strand, within RPSI07_RS01415 at 384.448 kb on + strand, within RPSI07_RS01415 at 384.449 kb on - strand, within RPSI07_RS01415 at 384.449 kb on - strand, within RPSI07_RS01415 at 384.449 kb on - strand, within RPSI07_RS01415 at 384.449 kb on - strand, within RPSI07_RS01415 at 384.514 kb on + strand at 384.515 kb on - strand at 384.515 kb on - strand at 384.578 kb on - strand at 384.597 kb on + strand at 384.597 kb on + strand at 384.597 kb on + strand at 384.598 kb on - strand at 384.598 kb on - strand at 384.807 kb on + strand, within RPSI07_RS01420 at 384.808 kb on - strand, within RPSI07_RS01420 at 384.900 kb on - strand, within RPSI07_RS01420 at 385.092 kb on - strand, within RPSI07_RS01420 at 385.267 kb on - strand, within RPSI07_RS01420 at 385.281 kb on + strand at 385.281 kb on + strand at 385.281 kb on + strand at 385.385 kb on + strand at 385.572 kb on + strand, within RPSI07_RS01425 at 385.573 kb on - strand, within RPSI07_RS01425 at 385.740 kb on + strand, within RPSI07_RS01425 at 385.783 kb on + strand, within RPSI07_RS01425 at 385.784 kb on - strand, within RPSI07_RS01425 at 385.784 kb on - strand, within RPSI07_RS01425 at 385.784 kb on - strand, within RPSI07_RS01425 at 385.784 kb on - strand, within RPSI07_RS01425
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 383,932 + RPSI07_RS01415 0.17 +0.5 383,933 - RPSI07_RS01415 0.17 -0.1 384,136 + RPSI07_RS01415 0.43 -0.2 384,448 + RPSI07_RS01415 0.83 +0.6 384,448 + RPSI07_RS01415 0.83 -2.3 384,448 + RPSI07_RS01415 0.83 +0.7 384,448 + RPSI07_RS01415 0.83 +2.0 384,448 + RPSI07_RS01415 0.83 +2.0 384,449 - RPSI07_RS01415 0.83 +1.2 384,449 - RPSI07_RS01415 0.83 +0.3 384,449 - RPSI07_RS01415 0.83 -0.6 384,449 - RPSI07_RS01415 0.83 +2.0 384,514 + -0.8 384,515 - -0.3 384,515 - +0.0 384,578 - -2.1 384,597 + +0.8 384,597 + -1.0 384,597 + +2.6 384,598 - +1.0 384,598 - -0.3 384,807 + RPSI07_RS01420 0.27 +0.6 384,808 - RPSI07_RS01420 0.27 -1.7 384,900 - RPSI07_RS01420 0.39 -0.9 385,092 - RPSI07_RS01420 0.65 -0.6 385,267 - RPSI07_RS01420 0.89 -0.2 385,281 + -0.8 385,281 + +2.0 385,281 + +0.1 385,385 + -0.6 385,572 + RPSI07_RS01425 0.18 +0.8 385,573 - RPSI07_RS01425 0.18 -1.0 385,740 + RPSI07_RS01425 0.33 -2.4 385,783 + RPSI07_RS01425 0.37 +0.3 385,784 - RPSI07_RS01425 0.37 -0.5 385,784 - RPSI07_RS01425 0.37 -2.0 385,784 - RPSI07_RS01425 0.37 +0.7 385,784 - RPSI07_RS01425 0.37 +0.5
Or see this region's nucleotide sequence