Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS01385 and RPSI07_RS01390 are separated by 413 nucleotides RPSI07_RS01390 and RPSI07_RS01395 are separated by 161 nucleotides RPSI07_RS01395 and RPSI07_RS01400 are separated by 29 nucleotides
RPSI07_RS01385: RPSI07_RS01385 - flagella basal body P-ring formation protein FlgA, at 378,749 to 379,630
_RS01385
RPSI07_RS01390: RPSI07_RS01390 - flagellar basal body rod protein FlgB, at 380,044 to 380,505
_RS01390
RPSI07_RS01395: RPSI07_RS01395 - flagellar basal body rod protein FlgC, at 380,667 to 381,074
_RS01395
RPSI07_RS01400: RPSI07_RS01400 - flagellar basal body rod modification protein FlgD, at 381,104 to 381,760
_RS01400
Position (kb)
380
381 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.116 kb on + strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.117 kb on - strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.119 kb on + strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.120 kb on - strand, within RPSI07_RS01385 at 379.196 kb on + strand, within RPSI07_RS01385 at 379.196 kb on + strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.197 kb on - strand, within RPSI07_RS01385 at 379.663 kb on + strand at 379.664 kb on - strand at 379.703 kb on + strand at 379.716 kb on - strand at 379.716 kb on - strand at 379.716 kb on - strand at 379.748 kb on - strand at 379.748 kb on - strand at 379.909 kb on - strand at 379.916 kb on + strand at 379.916 kb on + strand at 379.916 kb on + strand at 379.916 kb on + strand at 379.917 kb on - strand at 379.997 kb on - strand at 380.155 kb on + strand, within RPSI07_RS01390 at 380.155 kb on + strand, within RPSI07_RS01390 at 380.155 kb on + strand, within RPSI07_RS01390 at 380.155 kb on + strand, within RPSI07_RS01390 at 380.156 kb on - strand, within RPSI07_RS01390 at 380.156 kb on - strand, within RPSI07_RS01390 at 380.156 kb on - strand, within RPSI07_RS01390 at 380.156 kb on - strand, within RPSI07_RS01390 at 380.285 kb on + strand, within RPSI07_RS01390 at 380.285 kb on + strand, within RPSI07_RS01390 at 380.285 kb on + strand, within RPSI07_RS01390 at 380.286 kb on - strand, within RPSI07_RS01390 at 380.503 kb on + strand at 380.504 kb on - strand at 380.533 kb on - strand at 380.639 kb on + strand at 380.675 kb on - strand at 380.675 kb on - strand at 380.683 kb on + strand at 380.683 kb on + strand at 380.684 kb on - strand at 380.838 kb on + strand, within RPSI07_RS01395 at 380.839 kb on - strand, within RPSI07_RS01395 at 380.839 kb on - strand, within RPSI07_RS01395 at 380.937 kb on + strand, within RPSI07_RS01395
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 379,116 + RPSI07_RS01385 0.42 -1.1 379,116 + RPSI07_RS01385 0.42 -0.1 379,116 + RPSI07_RS01385 0.42 +0.9 379,116 + RPSI07_RS01385 0.42 -0.8 379,116 + RPSI07_RS01385 0.42 +0.4 379,116 + RPSI07_RS01385 0.42 -0.1 379,116 + RPSI07_RS01385 0.42 +0.1 379,116 + RPSI07_RS01385 0.42 -0.2 379,116 + RPSI07_RS01385 0.42 -0.1 379,116 + RPSI07_RS01385 0.42 -0.5 379,117 - RPSI07_RS01385 0.42 +0.3 379,117 - RPSI07_RS01385 0.42 +0.2 379,117 - RPSI07_RS01385 0.42 -0.6 379,117 - RPSI07_RS01385 0.42 +0.7 379,117 - RPSI07_RS01385 0.42 -1.0 379,117 - RPSI07_RS01385 0.42 +0.3 379,119 + RPSI07_RS01385 0.42 +0.6 379,119 + RPSI07_RS01385 0.42 +0.3 379,119 + RPSI07_RS01385 0.42 -1.3 379,119 + RPSI07_RS01385 0.42 +0.1 379,119 + RPSI07_RS01385 0.42 +2.8 379,119 + RPSI07_RS01385 0.42 -0.3 379,119 + RPSI07_RS01385 0.42 +0.7 379,120 - RPSI07_RS01385 0.42 +0.3 379,120 - RPSI07_RS01385 0.42 +0.0 379,120 - RPSI07_RS01385 0.42 +0.3 379,120 - RPSI07_RS01385 0.42 +0.3 379,120 - RPSI07_RS01385 0.42 +0.1 379,120 - RPSI07_RS01385 0.42 +0.2 379,120 - RPSI07_RS01385 0.42 -1.3 379,120 - RPSI07_RS01385 0.42 -0.7 379,196 + RPSI07_RS01385 0.51 -2.1 379,196 + RPSI07_RS01385 0.51 +0.1 379,197 - RPSI07_RS01385 0.51 +1.2 379,197 - RPSI07_RS01385 0.51 +2.0 379,197 - RPSI07_RS01385 0.51 +1.0 379,197 - RPSI07_RS01385 0.51 +0.6 379,197 - RPSI07_RS01385 0.51 -0.3 379,197 - RPSI07_RS01385 0.51 -0.3 379,197 - RPSI07_RS01385 0.51 -0.4 379,663 + +2.0 379,664 - +1.6 379,703 + +2.7 379,716 - -0.3 379,716 - +0.1 379,716 - +1.0 379,748 - -0.2 379,748 - -1.4 379,909 - -0.1 379,916 + -1.7 379,916 + -2.3 379,916 + +0.2 379,916 + +1.0 379,917 - -1.1 379,997 - -1.8 380,155 + RPSI07_RS01390 0.24 +1.8 380,155 + RPSI07_RS01390 0.24 -0.4 380,155 + RPSI07_RS01390 0.24 -0.3 380,155 + RPSI07_RS01390 0.24 -0.6 380,156 - RPSI07_RS01390 0.24 -2.6 380,156 - RPSI07_RS01390 0.24 -1.2 380,156 - RPSI07_RS01390 0.24 +1.8 380,156 - RPSI07_RS01390 0.24 +0.3 380,285 + RPSI07_RS01390 0.52 +0.1 380,285 + RPSI07_RS01390 0.52 +0.2 380,285 + RPSI07_RS01390 0.52 +0.6 380,286 - RPSI07_RS01390 0.52 +0.1 380,503 + -0.2 380,504 - +0.9 380,533 - -1.1 380,639 + -0.1 380,675 - -0.7 380,675 - +0.5 380,683 + +1.9 380,683 + -0.1 380,684 - +2.5 380,838 + RPSI07_RS01395 0.42 +1.5 380,839 - RPSI07_RS01395 0.42 +0.4 380,839 - RPSI07_RS01395 0.42 +0.3 380,937 + RPSI07_RS01395 0.66 +0.0
Or see this region's nucleotide sequence