Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07025 and RPSI07_RS24710 are separated by 259 nucleotides RPSI07_RS24710 and RPSI07_RS07030 are separated by 13 nucleotides
RPSI07_RS07025: RPSI07_RS07025 - hypothetical protein, at 1,864,769 to 1,865,125
_RS07025
RPSI07_RS24710: RPSI07_RS24710 - hypothetical protein, at 1,865,385 to 1,865,573
_RS24710
RPSI07_RS07030: RPSI07_RS07030 - hypothetical protein, at 1,865,587 to 1,866,315
_RS07030
Position (kb)
1865
1866 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1864.422 kb on + strand at 1864.422 kb on + strand at 1864.422 kb on + strand at 1864.422 kb on + strand at 1864.423 kb on - strand at 1864.423 kb on - strand at 1864.442 kb on - strand at 1864.442 kb on - strand at 1864.496 kb on + strand at 1864.496 kb on + strand at 1864.496 kb on + strand at 1864.496 kb on + strand at 1864.497 kb on - strand at 1864.497 kb on - strand at 1864.497 kb on - strand at 1864.537 kb on - strand at 1864.537 kb on - strand at 1864.537 kb on - strand at 1864.573 kb on + strand at 1864.573 kb on + strand at 1864.574 kb on - strand at 1864.574 kb on - strand at 1864.610 kb on + strand at 1864.659 kb on + strand at 1864.659 kb on + strand at 1864.660 kb on - strand at 1864.660 kb on - strand at 1864.684 kb on + strand at 1864.685 kb on - strand at 1864.685 kb on - strand at 1864.770 kb on + strand at 1864.777 kb on + strand at 1864.778 kb on - strand at 1864.778 kb on - strand at 1864.778 kb on - strand at 1864.778 kb on - strand at 1864.778 kb on - strand at 1864.797 kb on + strand at 1864.797 kb on + strand at 1864.797 kb on + strand at 1864.797 kb on + strand at 1864.798 kb on - strand at 1864.798 kb on - strand at 1864.798 kb on - strand at 1864.944 kb on + strand, within RPSI07_RS07025 at 1864.944 kb on + strand, within RPSI07_RS07025 at 1864.944 kb on + strand, within RPSI07_RS07025 at 1864.944 kb on + strand, within RPSI07_RS07025 at 1864.944 kb on + strand, within RPSI07_RS07025 at 1864.944 kb on + strand, within RPSI07_RS07025 at 1864.945 kb on - strand, within RPSI07_RS07025 at 1864.945 kb on - strand, within RPSI07_RS07025 at 1864.945 kb on - strand at 1864.945 kb on - strand, within RPSI07_RS07025 at 1864.945 kb on - strand, within RPSI07_RS07025 at 1864.945 kb on - strand, within RPSI07_RS07025 at 1864.987 kb on + strand, within RPSI07_RS07025 at 1864.987 kb on + strand, within RPSI07_RS07025 at 1865.036 kb on - strand, within RPSI07_RS07025 at 1865.107 kb on + strand at 1865.136 kb on - strand at 1865.136 kb on - strand at 1865.205 kb on - strand at 1865.205 kb on - strand at 1865.244 kb on + strand at 1865.296 kb on + strand at 1865.297 kb on - strand at 1865.299 kb on + strand at 1865.299 kb on + strand at 1865.365 kb on + strand at 1865.366 kb on - strand at 1865.366 kb on - strand at 1865.366 kb on - strand at 1865.366 kb on - strand at 1865.462 kb on + strand, within RPSI07_RS24710 at 1865.462 kb on + strand, within RPSI07_RS24710 at 1865.462 kb on + strand, within RPSI07_RS24710 at 1865.462 kb on + strand, within RPSI07_RS24710 at 1865.462 kb on + strand, within RPSI07_RS24710 at 1865.462 kb on + strand, within RPSI07_RS24710 at 1865.462 kb on + strand, within RPSI07_RS24710 at 1865.463 kb on - strand, within RPSI07_RS24710 at 1865.463 kb on - strand, within RPSI07_RS24710 at 1865.463 kb on - strand, within RPSI07_RS24710 at 1865.463 kb on - strand, within RPSI07_RS24710 at 1865.463 kb on - strand, within RPSI07_RS24710 at 1865.463 kb on - strand, within RPSI07_RS24710 at 1865.463 kb on - strand, within RPSI07_RS24710 at 1865.510 kb on + strand, within RPSI07_RS24710 at 1865.681 kb on + strand, within RPSI07_RS07030 at 1865.681 kb on + strand, within RPSI07_RS07030 at 1865.681 kb on + strand, within RPSI07_RS07030 at 1865.681 kb on + strand, within RPSI07_RS07030 at 1865.681 kb on + strand, within RPSI07_RS07030 at 1865.681 kb on + strand, within RPSI07_RS07030 at 1865.681 kb on + strand, within RPSI07_RS07030 at 1865.681 kb on + strand, within RPSI07_RS07030 at 1865.681 kb on + strand, within RPSI07_RS07030 at 1865.681 kb on + strand, within RPSI07_RS07030 at 1865.682 kb on - strand, within RPSI07_RS07030 at 1865.682 kb on - strand, within RPSI07_RS07030 at 1865.682 kb on - strand, within RPSI07_RS07030 at 1865.699 kb on + strand, within RPSI07_RS07030 at 1865.712 kb on + strand, within RPSI07_RS07030 at 1865.713 kb on - strand, within RPSI07_RS07030 at 1865.924 kb on - strand, within RPSI07_RS07030 at 1865.972 kb on + strand, within RPSI07_RS07030 at 1866.299 kb on + strand at 1866.375 kb on + strand at 1866.376 kb on + strand at 1866.377 kb on - strand at 1866.377 kb on - strand at 1866.378 kb on + strand at 1866.378 kb on + strand at 1866.378 kb on + strand at 1866.378 kb on + strand at 1866.379 kb on - strand at 1866.379 kb on - strand at 1866.388 kb on + strand at 1866.388 kb on + strand at 1866.388 kb on + strand at 1866.388 kb on + strand at 1866.388 kb on + strand at 1866.389 kb on - strand at 1866.389 kb on - strand at 1866.389 kb on - strand at 1866.389 kb on - strand at 1866.389 kb on - strand at 1866.389 kb on - strand at 1866.389 kb on - strand at 1866.443 kb on + strand at 1866.443 kb on + strand at 1866.444 kb on - strand at 1866.444 kb on - strand at 1866.444 kb on - strand at 1866.446 kb on + strand at 1866.446 kb on + strand at 1866.446 kb on + strand at 1866.447 kb on - strand at 1866.543 kb on - strand at 1866.564 kb on + strand at 1866.564 kb on + strand at 1866.565 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 1,864,422 + -0.5 1,864,422 + +0.7 1,864,422 + +0.8 1,864,422 + -0.4 1,864,423 - -0.3 1,864,423 - +0.6 1,864,442 - +0.9 1,864,442 - +1.9 1,864,496 + +0.6 1,864,496 + -1.0 1,864,496 + +5.2 1,864,496 + -0.8 1,864,497 - -0.2 1,864,497 - -1.4 1,864,497 - +0.2 1,864,537 - +1.5 1,864,537 - -2.4 1,864,537 - -0.3 1,864,573 + +1.2 1,864,573 + +0.2 1,864,574 - -2.1 1,864,574 - +0.9 1,864,610 + -0.8 1,864,659 + +1.2 1,864,659 + +0.4 1,864,660 - -0.7 1,864,660 - +1.9 1,864,684 + -1.1 1,864,685 - -0.0 1,864,685 - -0.1 1,864,770 + +0.5 1,864,777 + -0.1 1,864,778 - +0.2 1,864,778 - +0.6 1,864,778 - +0.6 1,864,778 - +0.9 1,864,778 - +0.9 1,864,797 + -0.6 1,864,797 + +0.0 1,864,797 + -0.1 1,864,797 + +0.1 1,864,798 - +1.9 1,864,798 - +1.1 1,864,798 - -0.4 1,864,944 + RPSI07_RS07025 0.49 -0.6 1,864,944 + RPSI07_RS07025 0.49 +0.6 1,864,944 + RPSI07_RS07025 0.49 -0.2 1,864,944 + RPSI07_RS07025 0.49 -0.1 1,864,944 + RPSI07_RS07025 0.49 +0.9 1,864,944 + RPSI07_RS07025 0.49 -1.2 1,864,945 - RPSI07_RS07025 0.49 -0.2 1,864,945 - RPSI07_RS07025 0.49 -0.8 1,864,945 - -0.9 1,864,945 - RPSI07_RS07025 0.49 -0.7 1,864,945 - RPSI07_RS07025 0.49 -2.9 1,864,945 - RPSI07_RS07025 0.49 -0.9 1,864,987 + RPSI07_RS07025 0.61 +0.6 1,864,987 + RPSI07_RS07025 0.61 +1.7 1,865,036 - RPSI07_RS07025 0.75 +1.0 1,865,107 + +0.4 1,865,136 - -1.3 1,865,136 - +0.2 1,865,205 - -0.1 1,865,205 - +0.1 1,865,244 + -0.0 1,865,296 + +0.1 1,865,297 - +3.3 1,865,299 + +0.2 1,865,299 + +1.6 1,865,365 + -1.8 1,865,366 - +0.9 1,865,366 - -0.3 1,865,366 - +0.2 1,865,366 - +0.5 1,865,462 + RPSI07_RS24710 0.41 -2.1 1,865,462 + RPSI07_RS24710 0.41 +1.9 1,865,462 + RPSI07_RS24710 0.41 -0.1 1,865,462 + RPSI07_RS24710 0.41 -0.8 1,865,462 + RPSI07_RS24710 0.41 -0.3 1,865,462 + RPSI07_RS24710 0.41 -0.2 1,865,462 + RPSI07_RS24710 0.41 -0.5 1,865,463 - RPSI07_RS24710 0.41 +0.5 1,865,463 - RPSI07_RS24710 0.41 +0.9 1,865,463 - RPSI07_RS24710 0.41 -0.4 1,865,463 - RPSI07_RS24710 0.41 -0.2 1,865,463 - RPSI07_RS24710 0.41 +1.5 1,865,463 - RPSI07_RS24710 0.41 +0.4 1,865,463 - RPSI07_RS24710 0.41 -0.6 1,865,510 + RPSI07_RS24710 0.66 -0.7 1,865,681 + RPSI07_RS07030 0.13 -0.2 1,865,681 + RPSI07_RS07030 0.13 +0.0 1,865,681 + RPSI07_RS07030 0.13 -0.1 1,865,681 + RPSI07_RS07030 0.13 -1.5 1,865,681 + RPSI07_RS07030 0.13 -0.5 1,865,681 + RPSI07_RS07030 0.13 -1.1 1,865,681 + RPSI07_RS07030 0.13 -1.5 1,865,681 + RPSI07_RS07030 0.13 +0.2 1,865,681 + RPSI07_RS07030 0.13 -1.3 1,865,681 + RPSI07_RS07030 0.13 -0.4 1,865,682 - RPSI07_RS07030 0.13 -0.2 1,865,682 - RPSI07_RS07030 0.13 -0.1 1,865,682 - RPSI07_RS07030 0.13 +0.9 1,865,699 + RPSI07_RS07030 0.15 +0.2 1,865,712 + RPSI07_RS07030 0.17 -2.3 1,865,713 - RPSI07_RS07030 0.17 -1.5 1,865,924 - RPSI07_RS07030 0.46 -1.2 1,865,972 + RPSI07_RS07030 0.53 -1.5 1,866,299 + +1.0 1,866,375 + -1.4 1,866,376 + -0.7 1,866,377 - -0.5 1,866,377 - +0.5 1,866,378 + -0.0 1,866,378 + -0.4 1,866,378 + +0.8 1,866,378 + -0.5 1,866,379 - +0.3 1,866,379 - -0.2 1,866,388 + -3.4 1,866,388 + -0.1 1,866,388 + -0.7 1,866,388 + -0.5 1,866,388 + -2.0 1,866,389 - -1.4 1,866,389 - -1.9 1,866,389 - -0.6 1,866,389 - +0.4 1,866,389 - -0.6 1,866,389 - +0.3 1,866,389 - -0.8 1,866,443 + -0.6 1,866,443 + -0.7 1,866,444 - -0.2 1,866,444 - +0.1 1,866,444 - +0.2 1,866,446 + +0.9 1,866,446 + +1.9 1,866,446 + -0.5 1,866,447 - +0.5 1,866,543 - -0.1 1,866,564 + -0.5 1,866,564 + -2.2 1,866,565 - +1.5
Or see this region's nucleotide sequence