Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22675 and RPSI07_RS22680 are separated by 84 nucleotides RPSI07_RS22680 and RPSI07_RS22685 overlap by 35 nucleotides
RPSI07_RS22675: RPSI07_RS22675 - FAA hydrolase family protein, at 3,238,695 to 3,239,687
_RS22675
RPSI07_RS22680: RPSI07_RS22680 - IclR family transcriptional regulator, at 3,239,772 to 3,240,644
_RS22680
RPSI07_RS22685: RPSI07_RS22685 - DUF3108 domain-containing protein, at 3,240,610 to 3,241,737
_RS22685
Position (kb)
3239
3240
3241 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3238.793 kb on - strand at 3238.856 kb on + strand, within RPSI07_RS22675 at 3238.864 kb on + strand, within RPSI07_RS22675 at 3238.865 kb on - strand, within RPSI07_RS22675 at 3239.347 kb on + strand, within RPSI07_RS22675 at 3239.347 kb on + strand, within RPSI07_RS22675 at 3239.348 kb on - strand, within RPSI07_RS22675 at 3239.348 kb on - strand, within RPSI07_RS22675 at 3239.425 kb on + strand, within RPSI07_RS22675 at 3239.426 kb on - strand, within RPSI07_RS22675 at 3239.426 kb on - strand, within RPSI07_RS22675 at 3239.449 kb on + strand, within RPSI07_RS22675 at 3239.450 kb on - strand, within RPSI07_RS22675 at 3239.530 kb on + strand, within RPSI07_RS22675 at 3239.531 kb on - strand, within RPSI07_RS22675 at 3239.554 kb on + strand, within RPSI07_RS22675 at 3239.554 kb on + strand, within RPSI07_RS22675 at 3239.555 kb on - strand, within RPSI07_RS22675 at 3239.693 kb on + strand at 3239.693 kb on + strand at 3239.693 kb on + strand at 3239.694 kb on - strand at 3239.694 kb on - strand at 3239.694 kb on - strand at 3239.723 kb on + strand at 3239.724 kb on - strand at 3239.724 kb on - strand at 3239.724 kb on - strand at 3239.724 kb on - strand at 3239.724 kb on - strand at 3239.724 kb on - strand at 3239.724 kb on - strand at 3239.740 kb on - strand at 3239.976 kb on + strand, within RPSI07_RS22680 at 3240.282 kb on + strand, within RPSI07_RS22680 at 3240.282 kb on + strand, within RPSI07_RS22680 at 3240.283 kb on - strand, within RPSI07_RS22680 at 3240.390 kb on + strand, within RPSI07_RS22680 at 3240.391 kb on - strand, within RPSI07_RS22680 at 3240.391 kb on - strand, within RPSI07_RS22680 at 3240.391 kb on - strand, within RPSI07_RS22680 at 3240.392 kb on - strand, within RPSI07_RS22680 at 3240.546 kb on - strand, within RPSI07_RS22680 at 3241.072 kb on + strand, within RPSI07_RS22685 at 3241.072 kb on + strand, within RPSI07_RS22685 at 3241.072 kb on + strand, within RPSI07_RS22685 at 3241.081 kb on + strand, within RPSI07_RS22685 at 3241.081 kb on + strand, within RPSI07_RS22685 at 3241.081 kb on + strand, within RPSI07_RS22685 at 3241.081 kb on + strand, within RPSI07_RS22685 at 3241.081 kb on + strand, within RPSI07_RS22685 at 3241.082 kb on - strand, within RPSI07_RS22685 at 3241.082 kb on - strand, within RPSI07_RS22685 at 3241.142 kb on - strand, within RPSI07_RS22685 at 3241.240 kb on + strand, within RPSI07_RS22685 at 3241.240 kb on + strand, within RPSI07_RS22685 at 3241.241 kb on - strand, within RPSI07_RS22685
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 3,238,793 - +0.1 3,238,856 + RPSI07_RS22675 0.16 +1.1 3,238,864 + RPSI07_RS22675 0.17 -2.4 3,238,865 - RPSI07_RS22675 0.17 +0.2 3,239,347 + RPSI07_RS22675 0.66 -0.4 3,239,347 + RPSI07_RS22675 0.66 +0.2 3,239,348 - RPSI07_RS22675 0.66 +0.1 3,239,348 - RPSI07_RS22675 0.66 +1.2 3,239,425 + RPSI07_RS22675 0.74 +2.6 3,239,426 - RPSI07_RS22675 0.74 +1.1 3,239,426 - RPSI07_RS22675 0.74 -1.8 3,239,449 + RPSI07_RS22675 0.76 +0.1 3,239,450 - RPSI07_RS22675 0.76 -0.5 3,239,530 + RPSI07_RS22675 0.84 -0.7 3,239,531 - RPSI07_RS22675 0.84 -1.3 3,239,554 + RPSI07_RS22675 0.87 +0.4 3,239,554 + RPSI07_RS22675 0.87 +3.2 3,239,555 - RPSI07_RS22675 0.87 +0.7 3,239,693 + -2.1 3,239,693 + +0.9 3,239,693 + -1.8 3,239,694 - +0.4 3,239,694 - -1.1 3,239,694 - +0.2 3,239,723 + +1.8 3,239,724 - -0.4 3,239,724 - -0.5 3,239,724 - +1.8 3,239,724 - +1.2 3,239,724 - -0.4 3,239,724 - -0.1 3,239,724 - +0.9 3,239,740 - -1.8 3,239,976 + RPSI07_RS22680 0.23 -1.3 3,240,282 + RPSI07_RS22680 0.58 -0.3 3,240,282 + RPSI07_RS22680 0.58 -1.6 3,240,283 - RPSI07_RS22680 0.59 +1.4 3,240,390 + RPSI07_RS22680 0.71 -2.4 3,240,391 - RPSI07_RS22680 0.71 -0.1 3,240,391 - RPSI07_RS22680 0.71 +0.9 3,240,391 - RPSI07_RS22680 0.71 +0.2 3,240,392 - RPSI07_RS22680 0.71 +1.2 3,240,546 - RPSI07_RS22680 0.89 +0.1 3,241,072 + RPSI07_RS22685 0.41 -0.8 3,241,072 + RPSI07_RS22685 0.41 -0.9 3,241,072 + RPSI07_RS22685 0.41 -0.5 3,241,081 + RPSI07_RS22685 0.42 +0.2 3,241,081 + RPSI07_RS22685 0.42 +0.4 3,241,081 + RPSI07_RS22685 0.42 -0.8 3,241,081 + RPSI07_RS22685 0.42 -0.4 3,241,081 + RPSI07_RS22685 0.42 +0.9 3,241,082 - RPSI07_RS22685 0.42 -1.4 3,241,082 - RPSI07_RS22685 0.42 +0.5 3,241,142 - RPSI07_RS22685 0.47 -1.1 3,241,240 + RPSI07_RS22685 0.56 +2.0 3,241,240 + RPSI07_RS22685 0.56 -0.7 3,241,241 - RPSI07_RS22685 0.56 -0.5
Or see this region's nucleotide sequence