Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21815 and RPSI07_RS21820 are separated by 228 nucleotides RPSI07_RS21820 and RPSI07_RS21825 are separated by 16 nucleotides RPSI07_RS21825 and RPSI07_RS21830 are separated by 25 nucleotides
RPSI07_RS21815: RPSI07_RS21815 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 3,057,108 to 3,057,941
_RS21815
RPSI07_RS21820: RPSI07_RS21820 - acetate kinase, at 3,058,170 to 3,059,510
_RS21820
RPSI07_RS21825: RPSI07_RS21825 - hypothetical protein, at 3,059,527 to 3,060,081
_RS21825
RPSI07_RS21830: RPSI07_RS21830 - peptidase C39, at 3,060,107 to 3,060,811
_RS21830
Position (kb)
3058
3059
3060 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3057.570 kb on + strand, within RPSI07_RS21815 at 3057.570 kb on + strand, within RPSI07_RS21815 at 3057.571 kb on - strand, within RPSI07_RS21815 at 3057.571 kb on - strand, within RPSI07_RS21815 at 3057.571 kb on - strand, within RPSI07_RS21815 at 3057.843 kb on + strand, within RPSI07_RS21815 at 3057.843 kb on + strand at 3057.844 kb on - strand, within RPSI07_RS21815 at 3057.853 kb on - strand, within RPSI07_RS21815 at 3057.902 kb on + strand at 3057.902 kb on + strand at 3058.020 kb on + strand at 3058.021 kb on - strand at 3058.021 kb on - strand at 3058.021 kb on - strand at 3058.080 kb on - strand at 3058.081 kb on + strand at 3058.081 kb on + strand at 3058.082 kb on - strand at 3058.082 kb on - strand at 3058.645 kb on - strand, within RPSI07_RS21820 at 3058.677 kb on + strand, within RPSI07_RS21820 at 3058.678 kb on - strand, within RPSI07_RS21820 at 3058.806 kb on + strand, within RPSI07_RS21820 at 3058.806 kb on + strand, within RPSI07_RS21820 at 3058.806 kb on + strand, within RPSI07_RS21820 at 3058.807 kb on - strand, within RPSI07_RS21820 at 3058.807 kb on - strand, within RPSI07_RS21820 at 3058.807 kb on - strand, within RPSI07_RS21820 at 3058.812 kb on + strand, within RPSI07_RS21820 at 3058.812 kb on + strand, within RPSI07_RS21820 at 3058.812 kb on + strand, within RPSI07_RS21820 at 3058.812 kb on + strand, within RPSI07_RS21820 at 3058.813 kb on - strand, within RPSI07_RS21820 at 3058.813 kb on - strand, within RPSI07_RS21820 at 3058.813 kb on - strand, within RPSI07_RS21820 at 3058.813 kb on - strand, within RPSI07_RS21820 at 3058.956 kb on + strand, within RPSI07_RS21820 at 3058.956 kb on + strand, within RPSI07_RS21820 at 3058.956 kb on + strand, within RPSI07_RS21820 at 3058.956 kb on + strand, within RPSI07_RS21820 at 3058.957 kb on - strand, within RPSI07_RS21820 at 3058.957 kb on - strand, within RPSI07_RS21820 at 3058.957 kb on - strand, within RPSI07_RS21820 at 3058.957 kb on - strand, within RPSI07_RS21820 at 3058.957 kb on - strand, within RPSI07_RS21820 at 3059.121 kb on + strand, within RPSI07_RS21820 at 3059.121 kb on + strand, within RPSI07_RS21820 at 3059.121 kb on + strand, within RPSI07_RS21820 at 3059.122 kb on - strand, within RPSI07_RS21820 at 3059.122 kb on - strand, within RPSI07_RS21820 at 3059.122 kb on - strand, within RPSI07_RS21820 at 3059.160 kb on + strand, within RPSI07_RS21820 at 3059.161 kb on - strand, within RPSI07_RS21820 at 3059.407 kb on - strand at 3059.407 kb on - strand at 3059.508 kb on + strand at 3059.846 kb on + strand, within RPSI07_RS21825 at 3059.846 kb on + strand, within RPSI07_RS21825 at 3059.847 kb on - strand, within RPSI07_RS21825 at 3060.477 kb on + strand, within RPSI07_RS21830 at 3060.478 kb on - strand, within RPSI07_RS21830
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 3,057,570 + RPSI07_RS21815 0.55 -0.8 3,057,570 + RPSI07_RS21815 0.55 +1.2 3,057,571 - RPSI07_RS21815 0.56 -2.6 3,057,571 - RPSI07_RS21815 0.56 -1.6 3,057,571 - RPSI07_RS21815 0.56 +1.2 3,057,843 + RPSI07_RS21815 0.88 +1.8 3,057,843 + +1.2 3,057,844 - RPSI07_RS21815 0.88 +2.8 3,057,853 - RPSI07_RS21815 0.89 -0.4 3,057,902 + -0.6 3,057,902 + -0.3 3,058,020 + -0.4 3,058,021 - +0.7 3,058,021 - +3.5 3,058,021 - +2.3 3,058,080 - -1.1 3,058,081 + +1.6 3,058,081 + +0.3 3,058,082 - +0.9 3,058,082 - -0.0 3,058,645 - RPSI07_RS21820 0.35 +0.3 3,058,677 + RPSI07_RS21820 0.38 +0.5 3,058,678 - RPSI07_RS21820 0.38 -1.3 3,058,806 + RPSI07_RS21820 0.47 +1.5 3,058,806 + RPSI07_RS21820 0.47 -0.8 3,058,806 + RPSI07_RS21820 0.47 -0.1 3,058,807 - RPSI07_RS21820 0.48 +4.0 3,058,807 - RPSI07_RS21820 0.48 +0.3 3,058,807 - RPSI07_RS21820 0.48 -1.9 3,058,812 + RPSI07_RS21820 0.48 +0.6 3,058,812 + RPSI07_RS21820 0.48 +0.2 3,058,812 + RPSI07_RS21820 0.48 -0.5 3,058,812 + RPSI07_RS21820 0.48 +1.0 3,058,813 - RPSI07_RS21820 0.48 -0.6 3,058,813 - RPSI07_RS21820 0.48 -1.4 3,058,813 - RPSI07_RS21820 0.48 -1.6 3,058,813 - RPSI07_RS21820 0.48 +1.2 3,058,956 + RPSI07_RS21820 0.59 -1.3 3,058,956 + RPSI07_RS21820 0.59 -0.5 3,058,956 + RPSI07_RS21820 0.59 -0.9 3,058,956 + RPSI07_RS21820 0.59 +0.1 3,058,957 - RPSI07_RS21820 0.59 -0.5 3,058,957 - RPSI07_RS21820 0.59 -0.9 3,058,957 - RPSI07_RS21820 0.59 -0.8 3,058,957 - RPSI07_RS21820 0.59 +1.9 3,058,957 - RPSI07_RS21820 0.59 -0.8 3,059,121 + RPSI07_RS21820 0.71 +0.1 3,059,121 + RPSI07_RS21820 0.71 -0.2 3,059,121 + RPSI07_RS21820 0.71 -0.4 3,059,122 - RPSI07_RS21820 0.71 -1.4 3,059,122 - RPSI07_RS21820 0.71 -1.5 3,059,122 - RPSI07_RS21820 0.71 -4.1 3,059,160 + RPSI07_RS21820 0.74 -1.2 3,059,161 - RPSI07_RS21820 0.74 -0.9 3,059,407 - +0.4 3,059,407 - -1.3 3,059,508 + -0.4 3,059,846 + RPSI07_RS21825 0.57 -0.3 3,059,846 + RPSI07_RS21825 0.57 +0.1 3,059,847 - RPSI07_RS21825 0.58 +0.0 3,060,477 + RPSI07_RS21830 0.52 -3.4 3,060,478 - RPSI07_RS21830 0.53 +0.4
Or see this region's nucleotide sequence