Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21625 and RPSI07_RS21630 are separated by 36 nucleotides RPSI07_RS21630 and RPSI07_RS21635 are separated by 298 nucleotides
RPSI07_RS21625: RPSI07_RS21625 - dihydroorotase, at 3,010,311 to 3,011,345
_RS21625
RPSI07_RS21630: RPSI07_RS21630 - glycerate kinase, at 3,011,382 to 3,012,680
_RS21630
RPSI07_RS21635: RPSI07_RS21635 - class II glutamine amidotransferase, at 3,012,979 to 3,013,809
_RS21635
Position (kb)
3011
3012
3013 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3011.471 kb on - strand at 3011.471 kb on - strand at 3011.605 kb on + strand, within RPSI07_RS21630 at 3011.605 kb on + strand, within RPSI07_RS21630 at 3011.785 kb on + strand, within RPSI07_RS21630 at 3011.785 kb on + strand, within RPSI07_RS21630 at 3011.785 kb on + strand, within RPSI07_RS21630 at 3011.786 kb on - strand, within RPSI07_RS21630 at 3011.786 kb on - strand, within RPSI07_RS21630 at 3011.786 kb on - strand, within RPSI07_RS21630 at 3011.786 kb on - strand, within RPSI07_RS21630 at 3011.822 kb on + strand, within RPSI07_RS21630 at 3011.822 kb on + strand, within RPSI07_RS21630 at 3011.823 kb on - strand, within RPSI07_RS21630 at 3011.823 kb on - strand, within RPSI07_RS21630 at 3012.050 kb on - strand, within RPSI07_RS21630 at 3012.454 kb on + strand, within RPSI07_RS21630 at 3012.454 kb on + strand, within RPSI07_RS21630 at 3012.497 kb on - strand, within RPSI07_RS21630 at 3012.497 kb on - strand, within RPSI07_RS21630 at 3012.497 kb on - strand, within RPSI07_RS21630 at 3012.942 kb on - strand at 3012.948 kb on + strand at 3012.949 kb on - strand at 3012.953 kb on + strand at 3012.954 kb on - strand at 3013.215 kb on + strand, within RPSI07_RS21635 at 3013.215 kb on + strand, within RPSI07_RS21635 at 3013.447 kb on + strand, within RPSI07_RS21635 at 3013.447 kb on + strand, within RPSI07_RS21635 at 3013.448 kb on - strand, within RPSI07_RS21635 at 3013.498 kb on + strand, within RPSI07_RS21635 at 3013.498 kb on + strand, within RPSI07_RS21635 at 3013.499 kb on - strand, within RPSI07_RS21635 at 3013.499 kb on - strand, within RPSI07_RS21635 at 3013.549 kb on + strand, within RPSI07_RS21635 at 3013.549 kb on + strand, within RPSI07_RS21635 at 3013.550 kb on - strand, within RPSI07_RS21635 at 3013.552 kb on + strand, within RPSI07_RS21635 at 3013.552 kb on + strand, within RPSI07_RS21635 at 3013.624 kb on + strand, within RPSI07_RS21635 at 3013.624 kb on + strand, within RPSI07_RS21635 at 3013.624 kb on + strand, within RPSI07_RS21635 at 3013.625 kb on - strand, within RPSI07_RS21635
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 3,011,471 - +1.4 3,011,471 - -2.0 3,011,605 + RPSI07_RS21630 0.17 -0.5 3,011,605 + RPSI07_RS21630 0.17 +1.1 3,011,785 + RPSI07_RS21630 0.31 -0.4 3,011,785 + RPSI07_RS21630 0.31 -0.6 3,011,785 + RPSI07_RS21630 0.31 +0.9 3,011,786 - RPSI07_RS21630 0.31 +2.8 3,011,786 - RPSI07_RS21630 0.31 +1.5 3,011,786 - RPSI07_RS21630 0.31 +0.7 3,011,786 - RPSI07_RS21630 0.31 -0.3 3,011,822 + RPSI07_RS21630 0.34 +0.9 3,011,822 + RPSI07_RS21630 0.34 -0.5 3,011,823 - RPSI07_RS21630 0.34 -0.4 3,011,823 - RPSI07_RS21630 0.34 +0.1 3,012,050 - RPSI07_RS21630 0.51 -0.6 3,012,454 + RPSI07_RS21630 0.83 -0.8 3,012,454 + RPSI07_RS21630 0.83 -0.8 3,012,497 - RPSI07_RS21630 0.86 +1.1 3,012,497 - RPSI07_RS21630 0.86 -0.5 3,012,497 - RPSI07_RS21630 0.86 +0.3 3,012,942 - +2.1 3,012,948 + -1.2 3,012,949 - -0.0 3,012,953 + -0.4 3,012,954 - -1.2 3,013,215 + RPSI07_RS21635 0.28 +0.4 3,013,215 + RPSI07_RS21635 0.28 -0.8 3,013,447 + RPSI07_RS21635 0.56 -2.7 3,013,447 + RPSI07_RS21635 0.56 -0.2 3,013,448 - RPSI07_RS21635 0.56 +2.5 3,013,498 + RPSI07_RS21635 0.62 -2.0 3,013,498 + RPSI07_RS21635 0.62 +0.9 3,013,499 - RPSI07_RS21635 0.63 -1.6 3,013,499 - RPSI07_RS21635 0.63 +1.0 3,013,549 + RPSI07_RS21635 0.69 +0.1 3,013,549 + RPSI07_RS21635 0.69 +1.1 3,013,550 - RPSI07_RS21635 0.69 -0.6 3,013,552 + RPSI07_RS21635 0.69 +1.5 3,013,552 + RPSI07_RS21635 0.69 -0.6 3,013,624 + RPSI07_RS21635 0.78 -0.0 3,013,624 + RPSI07_RS21635 0.78 -1.5 3,013,624 + RPSI07_RS21635 0.78 -0.6 3,013,625 - RPSI07_RS21635 0.78 +1.1
Or see this region's nucleotide sequence