Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS12325 and RPSI07_RS12330 are separated by 95 nucleotides RPSI07_RS12330 and RPSI07_RS12335 are separated by 18 nucleotides RPSI07_RS12335 and RPSI07_RS12340 are separated by 660 nucleotides
RPSI07_RS12325: RPSI07_RS12325 - signal peptidase, at 960,203 to 961,219
_RS12325
RPSI07_RS12330: RPSI07_RS12330 - hypothetical protein, at 961,315 to 961,587
_RS12330
RPSI07_RS12335: RPSI07_RS12335 - type II toxin-antitoxin system HipA family toxin, at 961,606 to 962,931
_RS12335
RPSI07_RS12340: RPSI07_RS12340 - EamA/RhaT family transporter, at 963,592 to 964,524
_RS12340
Position (kb) 961
962
963 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 960.606 kb on - strand, within RPSI07_RS12325 at 960.606 kb on - strand, within RPSI07_RS12325 at 960.606 kb on - strand, within RPSI07_RS12325 at 960.606 kb on - strand, within RPSI07_RS12325 at 960.643 kb on + strand, within RPSI07_RS12325 at 960.644 kb on + strand, within RPSI07_RS12325 at 960.644 kb on + strand, within RPSI07_RS12325 at 960.644 kb on + strand, within RPSI07_RS12325 at 960.644 kb on + strand, within RPSI07_RS12325 at 960.644 kb on + strand, within RPSI07_RS12325 at 960.644 kb on + strand, within RPSI07_RS12325 at 960.645 kb on - strand, within RPSI07_RS12325 at 960.645 kb on - strand, within RPSI07_RS12325 at 960.645 kb on - strand, within RPSI07_RS12325 at 960.645 kb on - strand, within RPSI07_RS12325 at 960.645 kb on - strand, within RPSI07_RS12325 at 960.645 kb on - strand, within RPSI07_RS12325 at 960.645 kb on - strand, within RPSI07_RS12325 at 960.645 kb on - strand, within RPSI07_RS12325 at 960.645 kb on - strand, within RPSI07_RS12325 at 960.732 kb on - strand, within RPSI07_RS12325 at 960.732 kb on - strand, within RPSI07_RS12325 at 960.826 kb on + strand, within RPSI07_RS12325 at 960.826 kb on + strand, within RPSI07_RS12325 at 960.827 kb on - strand, within RPSI07_RS12325 at 960.827 kb on - strand, within RPSI07_RS12325 at 960.909 kb on - strand, within RPSI07_RS12325 at 960.950 kb on + strand, within RPSI07_RS12325 at 960.951 kb on - strand, within RPSI07_RS12325 at 960.951 kb on - strand, within RPSI07_RS12325 at 961.272 kb on + strand at 961.272 kb on + strand at 961.273 kb on - strand at 961.273 kb on - strand at 961.394 kb on + strand, within RPSI07_RS12330 at 961.450 kb on + strand, within RPSI07_RS12330 at 961.530 kb on + strand, within RPSI07_RS12330 at 961.530 kb on + strand, within RPSI07_RS12330 at 961.530 kb on + strand, within RPSI07_RS12330 at 961.530 kb on + strand, within RPSI07_RS12330 at 961.585 kb on + strand at 961.586 kb on - strand at 961.586 kb on - strand at 961.806 kb on - strand, within RPSI07_RS12335 at 961.806 kb on - strand, within RPSI07_RS12335 at 961.856 kb on + strand, within RPSI07_RS12335 at 961.857 kb on - strand, within RPSI07_RS12335 at 961.857 kb on - strand, within RPSI07_RS12335 at 961.928 kb on + strand, within RPSI07_RS12335 at 961.928 kb on + strand, within RPSI07_RS12335 at 961.928 kb on + strand, within RPSI07_RS12335 at 962.138 kb on + strand, within RPSI07_RS12335 at 962.138 kb on + strand, within RPSI07_RS12335 at 962.139 kb on - strand, within RPSI07_RS12335 at 962.139 kb on - strand, within RPSI07_RS12335 at 962.180 kb on + strand, within RPSI07_RS12335 at 962.181 kb on - strand, within RPSI07_RS12335 at 962.181 kb on - strand, within RPSI07_RS12335 at 962.346 kb on - strand, within RPSI07_RS12335 at 962.646 kb on + strand, within RPSI07_RS12335 at 962.647 kb on - strand, within RPSI07_RS12335 at 962.647 kb on - strand, within RPSI07_RS12335 at 962.741 kb on + strand, within RPSI07_RS12335 at 962.741 kb on + strand, within RPSI07_RS12335 at 962.742 kb on - strand, within RPSI07_RS12335 at 962.779 kb on - strand, within RPSI07_RS12335 at 962.779 kb on - strand, within RPSI07_RS12335 at 962.858 kb on + strand at 962.859 kb on - strand at 962.918 kb on + strand at 962.918 kb on + strand at 962.918 kb on + strand at 962.919 kb on - strand at 962.919 kb on - strand at 963.066 kb on - strand at 963.084 kb on - strand at 963.084 kb on - strand at 963.180 kb on + strand at 963.202 kb on + strand at 963.203 kb on - strand at 963.346 kb on + strand at 963.346 kb on + strand at 963.347 kb on - strand at 963.347 kb on - strand at 963.388 kb on + strand at 963.389 kb on - strand at 963.389 kb on - strand at 963.402 kb on + strand at 963.403 kb on - strand at 963.631 kb on - strand at 963.631 kb on - strand at 963.851 kb on + strand, within RPSI07_RS12340 at 963.851 kb on + strand, within RPSI07_RS12340 at 963.851 kb on + strand, within RPSI07_RS12340 at 963.851 kb on + strand, within RPSI07_RS12340 at 963.852 kb on - strand, within RPSI07_RS12340 at 963.852 kb on - strand, within RPSI07_RS12340 at 963.852 kb on - strand, within RPSI07_RS12340 at 963.852 kb on - strand, within RPSI07_RS12340 at 963.852 kb on - strand, within RPSI07_RS12340 at 963.852 kb on - strand, within RPSI07_RS12340 at 963.852 kb on - strand, within RPSI07_RS12340
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 960,606 - RPSI07_RS12325 0.40 +0.5 960,606 - RPSI07_RS12325 0.40 +1.1 960,606 - RPSI07_RS12325 0.40 +0.7 960,606 - RPSI07_RS12325 0.40 -1.3 960,643 + RPSI07_RS12325 0.43 +1.1 960,644 + RPSI07_RS12325 0.43 -0.4 960,644 + RPSI07_RS12325 0.43 +0.6 960,644 + RPSI07_RS12325 0.43 +1.1 960,644 + RPSI07_RS12325 0.43 -0.9 960,644 + RPSI07_RS12325 0.43 -0.9 960,644 + RPSI07_RS12325 0.43 -2.0 960,645 - RPSI07_RS12325 0.43 +0.9 960,645 - RPSI07_RS12325 0.43 +1.2 960,645 - RPSI07_RS12325 0.43 +0.1 960,645 - RPSI07_RS12325 0.43 -0.1 960,645 - RPSI07_RS12325 0.43 -1.9 960,645 - RPSI07_RS12325 0.43 -1.7 960,645 - RPSI07_RS12325 0.43 -0.3 960,645 - RPSI07_RS12325 0.43 +0.4 960,645 - RPSI07_RS12325 0.43 +0.0 960,732 - RPSI07_RS12325 0.52 -0.5 960,732 - RPSI07_RS12325 0.52 -0.0 960,826 + RPSI07_RS12325 0.61 -0.4 960,826 + RPSI07_RS12325 0.61 -0.7 960,827 - RPSI07_RS12325 0.61 -0.2 960,827 - RPSI07_RS12325 0.61 -0.9 960,909 - RPSI07_RS12325 0.69 -0.1 960,950 + RPSI07_RS12325 0.73 -1.3 960,951 - RPSI07_RS12325 0.74 +2.1 960,951 - RPSI07_RS12325 0.74 +0.9 961,272 + +0.1 961,272 + -1.5 961,273 - -2.9 961,273 - -0.1 961,394 + RPSI07_RS12330 0.29 -0.3 961,450 + RPSI07_RS12330 0.49 -1.0 961,530 + RPSI07_RS12330 0.79 -2.0 961,530 + RPSI07_RS12330 0.79 -0.5 961,530 + RPSI07_RS12330 0.79 -2.1 961,530 + RPSI07_RS12330 0.79 -1.7 961,585 + -1.5 961,586 - +1.1 961,586 - +2.7 961,806 - RPSI07_RS12335 0.15 +1.5 961,806 - RPSI07_RS12335 0.15 +0.4 961,856 + RPSI07_RS12335 0.19 +1.1 961,857 - RPSI07_RS12335 0.19 +1.4 961,857 - RPSI07_RS12335 0.19 +1.6 961,928 + RPSI07_RS12335 0.24 +1.5 961,928 + RPSI07_RS12335 0.24 +1.1 961,928 + RPSI07_RS12335 0.24 +1.4 962,138 + RPSI07_RS12335 0.40 -1.3 962,138 + RPSI07_RS12335 0.40 +1.1 962,139 - RPSI07_RS12335 0.40 +1.4 962,139 - RPSI07_RS12335 0.40 +1.9 962,180 + RPSI07_RS12335 0.43 +6.0 962,181 - RPSI07_RS12335 0.43 +1.6 962,181 - RPSI07_RS12335 0.43 +2.7 962,346 - RPSI07_RS12335 0.56 +1.8 962,646 + RPSI07_RS12335 0.78 +0.7 962,647 - RPSI07_RS12335 0.79 +1.8 962,647 - RPSI07_RS12335 0.79 +2.3 962,741 + RPSI07_RS12335 0.86 +1.7 962,741 + RPSI07_RS12335 0.86 +1.6 962,742 - RPSI07_RS12335 0.86 +1.4 962,779 - RPSI07_RS12335 0.88 +1.5 962,779 - RPSI07_RS12335 0.88 +2.2 962,858 + +2.1 962,859 - +1.1 962,918 + +1.4 962,918 + +1.9 962,918 + +1.6 962,919 - +1.5 962,919 - +2.1 963,066 - +3.2 963,084 - +1.4 963,084 - +1.1 963,180 + +1.5 963,202 + +0.7 963,203 - -0.3 963,346 + +0.8 963,346 + +0.8 963,347 - +0.1 963,347 - +0.5 963,388 + +1.1 963,389 - +1.5 963,389 - -0.9 963,402 + +1.6 963,403 - +1.1 963,631 - +1.1 963,631 - +0.4 963,851 + RPSI07_RS12340 0.28 -0.3 963,851 + RPSI07_RS12340 0.28 -0.1 963,851 + RPSI07_RS12340 0.28 +0.8 963,851 + RPSI07_RS12340 0.28 -0.8 963,852 - RPSI07_RS12340 0.28 +2.3 963,852 - RPSI07_RS12340 0.28 +0.7 963,852 - RPSI07_RS12340 0.28 -1.4 963,852 - RPSI07_RS12340 0.28 +0.6 963,852 - RPSI07_RS12340 0.28 +0.7 963,852 - RPSI07_RS12340 0.28 +0.8 963,852 - RPSI07_RS12340 0.28 +0.8
Or see this region's nucleotide sequence