Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11045 and RPSI07_RS11050 are separated by 12 nucleotides RPSI07_RS11050 and RPSI07_RS11055 are separated by 2 nucleotides RPSI07_RS11055 and RPSI07_RS11060 are separated by 44 nucleotides
RPSI07_RS11045: RPSI07_RS11045 - dephospho-CoA kinase, at 679,663 to 680,277
_RS11045
RPSI07_RS11050: RPSI07_RS11050 - prepilin peptidase, at 680,290 to 681,171
_RS11050
RPSI07_RS11055: RPSI07_RS11055 - type II secretion system F family protein, at 681,174 to 682,439
_RS11055
RPSI07_RS11060: RPSI07_RS11060 - type IV-A pilus assembly ATPase PilB, at 682,484 to 684,211
_RS11060
Position (kb) 681
682
683 Strain fitness (log2 ratio) -1
0
1
2 at 680.976 kb on - strand, within RPSI07_RS11050 at 680.976 kb on - strand, within RPSI07_RS11050 at 681.208 kb on + strand at 681.209 kb on - strand at 681.478 kb on + strand, within RPSI07_RS11055 at 681.478 kb on + strand, within RPSI07_RS11055 at 681.479 kb on - strand, within RPSI07_RS11055 at 681.479 kb on - strand, within RPSI07_RS11055 at 681.479 kb on - strand, within RPSI07_RS11055 at 681.561 kb on - strand, within RPSI07_RS11055 at 681.716 kb on - strand, within RPSI07_RS11055 at 681.716 kb on - strand, within RPSI07_RS11055 at 681.716 kb on - strand, within RPSI07_RS11055 at 681.874 kb on + strand, within RPSI07_RS11055 at 681.926 kb on - strand, within RPSI07_RS11055 at 681.926 kb on - strand, within RPSI07_RS11055 at 681.960 kb on - strand, within RPSI07_RS11055 at 681.995 kb on - strand, within RPSI07_RS11055 at 682.010 kb on - strand, within RPSI07_RS11055 at 682.184 kb on - strand, within RPSI07_RS11055 at 682.184 kb on - strand, within RPSI07_RS11055 at 682.358 kb on - strand at 682.358 kb on - strand at 682.358 kb on - strand at 682.358 kb on - strand at 682.662 kb on + strand, within RPSI07_RS11060 at 682.663 kb on - strand, within RPSI07_RS11060 at 683.182 kb on - strand, within RPSI07_RS11060 at 683.288 kb on + strand, within RPSI07_RS11060 at 683.288 kb on + strand, within RPSI07_RS11060 at 683.289 kb on - strand, within RPSI07_RS11060 at 683.289 kb on - strand, within RPSI07_RS11060 at 683.289 kb on - strand, within RPSI07_RS11060 at 683.289 kb on - strand, within RPSI07_RS11060
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 680,976 - RPSI07_RS11050 0.78 -0.5 680,976 - RPSI07_RS11050 0.78 -0.7 681,208 + -1.7 681,209 - +1.6 681,478 + RPSI07_RS11055 0.24 +1.2 681,478 + RPSI07_RS11055 0.24 +2.2 681,479 - RPSI07_RS11055 0.24 +1.0 681,479 - RPSI07_RS11055 0.24 +0.1 681,479 - RPSI07_RS11055 0.24 +0.8 681,561 - RPSI07_RS11055 0.31 +0.7 681,716 - RPSI07_RS11055 0.43 +0.2 681,716 - RPSI07_RS11055 0.43 +0.7 681,716 - RPSI07_RS11055 0.43 -1.0 681,874 + RPSI07_RS11055 0.55 -0.6 681,926 - RPSI07_RS11055 0.59 +1.4 681,926 - RPSI07_RS11055 0.59 +1.5 681,960 - RPSI07_RS11055 0.62 +1.2 681,995 - RPSI07_RS11055 0.65 +2.0 682,010 - RPSI07_RS11055 0.66 -1.2 682,184 - RPSI07_RS11055 0.80 +0.1 682,184 - RPSI07_RS11055 0.80 -0.6 682,358 - +0.3 682,358 - +0.5 682,358 - +0.6 682,358 - -0.1 682,662 + RPSI07_RS11060 0.10 -0.3 682,663 - RPSI07_RS11060 0.10 +1.4 683,182 - RPSI07_RS11060 0.40 +1.4 683,288 + RPSI07_RS11060 0.47 +0.1 683,288 + RPSI07_RS11060 0.47 +0.9 683,289 - RPSI07_RS11060 0.47 +0.0 683,289 - RPSI07_RS11060 0.47 +2.5 683,289 - RPSI07_RS11060 0.47 +0.3 683,289 - RPSI07_RS11060 0.47 -0.7
Or see this region's nucleotide sequence